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HOOK2 (NM_013312) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG201913
HOOK2 (tGFP-tagged) - Human hook homolog 2 (Drosophila) (HOOK2), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$789.00 $805.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HOOK2
Synonyms HK2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG201913 representing NM_013312
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGTGGACAAAGCTGAGCTATGCGGGTCTCTGCTCACCTGGTTACAGACGTTCCACGTTCCGTCTC
CCTGTGCCAGCCCTCAGGACCTGAGCAGCGGCCTTGCCGTAGCCTATGTGCTGAACCAGATAGACCCCTC
CTGGTTCAACGAGGCATGGCTCCAGGGCATCTCGGAAGATCCAGGTCCCAACTGGAAGCTGAAGGTCAGC
AATCTGAAGATGGTCTTACGGAGCCTAGTAGAGTACTCCCAGGATGTCCTGGCGCATCCTGTGTCAGAAG
AGCATCTCCCAGATGTGAGCCTCATTGGAGAGTTCTCAGACCCGGCAGAGCTCGGCAAGCTGCTTCAGCT
GGTGCTGGGCTGTGCCATCAGTTGCGAGAAAAAGCAGGACCACATCCAGAGAATCATGACGCTGGAAGAA
TCGGTTCAGCATGTGGTGATGGAAGCCATCCAAGAGCTCATGACCAAAGACACTCCTGACTCCCTGTCAC
CAGAGACGTATGGCAACTTTGACAGCCAGTCCCGCAGGTACTATTTCCTAAGTGAGGAGGCTGAGGAGGG
GGACGAATTACAGCAGCGCTGTCTGGATCTGGAGCGGCAGCTGATGCTCCTGTCAGAGGAGAAGCAGAGC
CTGGCGCAAGAGAATGCAGGGCTGCGGGAGCGGATGGGCCGGCCTGAAGGCGAGGGTACCCCAGGTCTCA
CTGCCAAGAAGCTGCTGCTGCTGCAATCCCAGCTGGAGCAGTTGCAGGAGGAGAACTTCAGGCTGGAGAG
TGGCAGGGAGGATGAGCGCCTGCGCTGTGCCGAGCTGGAGAGGGAGGTTGCGGAGCTGCAGCACCGGAAC
CAGGCGCTGACTAGCCTGGCCCAGGAGGCACAGGCCCTGAAGGATGAGATGGATGAACTACGGCAGTCTT
CGGAGCGTGCTGGGCAGCTGGAGGCCACGCTGACCAGTTGCCGGCGCCGCTTGGGCGAGCTGAGGGAGCT
GCGGCGGCAGGTGCGGCAGCTGGAGGAACGCAACGCCGGCCACGCCGAGCGCACGCGACAACTGGAGGAT
GAGCTACGCCGAGCGGGCTCCCTGCGCGCCCAGCTGGAGGCGCAGCGGCGGCAGGTGCAGGAACTGCAGG
GCCAGCGGCAGGAGGAGGCCATGAAGGCCGAGAAATGGCTATTTGAATGCCGCAACCTGGAGGAAAAGTA
TGAGTCGGTGACAAAGGAGAAGGAGCGGCTGTTGGCGGAGCGGGACTCCTTGCGGGAGGCCAATGAGGAG
CTGCGCTGCGCCCAGCTGCAGCCGCGGGGGTTGACCCAGGCCGATCCCTCACTGGATCCCACCTCCACAC
CCGTGGATAACTTAGCCGCAGAGATCCTGCCTGCGGAGCTCAGGGAGACGCTCCTGCGGCTTCAGCTGGA
GAACAAGCGGCTGTGCAGGCAGGAGGCGGCCGACCGGGAGCGGCAGGAGGAGCTGCAGCGCCAGCTGGAG
GATGCCAACCGCGCGCGCCACGGGTTGGAGACGCAGCACCGGCTGAACCAGCAGCAGCTATCCGAGCTGC
GGGCCCAGGTGGAGGACCTGCAGAAAGCCCTGCAGGAGCAGGGGGGCAAGACTGAAGATTCCATTTTGCT
GAAAAGGAAGCTGGAGGAACATTTGCAGAAGCTTCATGAGGCAGATCTGGAGTTGCAGAGGAAGCGGGAG
TACATTGAGGAGCTGGAGCCACCCACTGACAGCAGCACAGCCCGGCGGATCGAGGAGCTGCAGCATAACT
TGCAGAAGAAGGACGCGGACTTGCGGGCCATGGAGGAGCGATACCGCCGCTACGTGGACAAGGCCCGCAT
GGTCATGCAGACCATGGAACCCAAGCAGCGGCCAGCTGCGGGGGCACCTCCAGAACTCCATTCCCTGAGG
ACACAGCTCCGAGAACGGGATGTCCGCATCCGACACCTGGAGATGGACTTTGAGAAAAGCCGAAGTCAGC
GGGAGCAGGAAGAAAAGCTGCTCATCAGTGCCTGGTATAATATGGGCATGGCCTTGCAGCAGCGAGCTGG
GGAGGAGCGGGCGCCTGCCCATGCCCAGTCATTCCTGGCACAGCAGCGGCTGGCAACCAATTCTCGCCGT
GGACCCTTGGGACGCCTGGCATCTCTGAACCTTCGCCCCACTGACAAGCAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG201913 representing NM_013312
Red=Cloning site Green=Tags(s)

MSVDKAELCGSLLTWLQTFHVPSPCASPQDLSSGLAVAYVLNQIDPSWFNEAWLQGISEDPGPNWKLKVS
NLKMVLRSLVEYSQDVLAHPVSEEHLPDVSLIGEFSDPAELGKLLQLVLGCAISCEKKQDHIQRIMTLEE
SVQHVVMEAIQELMTKDTPDSLSPETYGNFDSQSRRYYFLSEEAEEGDELQQRCLDLERQLMLLSEEKQS
LAQENAGLRERMGRPEGEGTPGLTAKKLLLLQSQLEQLQEENFRLESGREDERLRCAELEREVAELQHRN
QALTSLAQEAQALKDEMDELRQSSERAGQLEATLTSCRRRLGELRELRRQVRQLEERNAGHAERTRQLED
ELRRAGSLRAQLEAQRRQVQELQGQRQEEAMKAEKWLFECRNLEEKYESVTKEKERLLAERDSLREANEE
LRCAQLQPRGLTQADPSLDPTSTPVDNLAAEILPAELRETLLRLQLENKRLCRQEAADRERQEELQRQLE
DANRARHGLETQHRLNQQQLSELRAQVEDLQKALQEQGGKTEDSILLKRKLEEHLQKLHEADLELQRKRE
YIEELEPPTDSSTARRIEELQHNLQKKDADLRAMEERYRRYVDKARMVMQTMEPKQRPAAGAPPELHSLR
TQLRERDVRIRHLEMDFEKSRSQREQEEKLLISAWYNMGMALQQRAGEERAPAHAQSFLAQQRLATNSRR
GPLGRLASLNLRPTDKH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_013312
ORF Size 2151 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_013312.1, NP_037444.1
RefSeq Size 2531 bp
RefSeq ORF 2160 bp
Locus ID 29911
UniProt ID Q96ED9
Cytogenetics 19p13.13
Summary Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.supplied by OMIM, Apr 2004
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"NM_013312" in other vectors (4)
SKU Description Size Price
RC201913 HOOK2 (Myc-DDK-tagged)-Human hook homolog 2 (Drosophila) (HOOK2), transcript variant 1 10 ug
$289.00 $605.00
RC201913L3 Lenti-ORF clone of HOOK2 (Myc-DDK-tagged)-Human hook homolog 2 (Drosophila) (HOOK2), transcript variant 1 10 ug
$905.00
RC201913L4 Lenti-ORF clone of HOOK2 (mGFP-tagged)-Human hook homolog 2 (Drosophila) (HOOK2), transcript variant 1 10 ug
$905.00
SC111255 HOOK2 (untagged)-Human hook homolog 2 (Drosophila) (HOOK2), transcript variant 1 10 ug
$659.00

Plasmid Map for RG201913

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG201913
Circular map for RG201913

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