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GPR162 (NM_014449) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG201764
GPR162 (tGFP-tagged) - Human G protein-coupled receptor 162 (GPR162), transcript variant A-1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $500.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GPR162
Synonyms A-2; GRCA
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG201764 representing NM_014449
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGAGCACTGGGGTGGTGAGCTTCTTCTCCCTCAAGTCGGACTCGGCGCCCCCCTGGATGGTGCTGG
CTGTGCTGTGGTGCTCCATGGCACAGACGCTGCTGCTGCCCTCCTTCATCTGGTCCTGCGAGCGCTACCG
CGCCGACGTGCGCACAGTGTGGGAGCAATGCGTGGCCATCATGTCTGAGGAGGATGGAGATGACGATGGG
GGCTGTGACGACTATGCAGAGGGCCGAGTTTGCAAAGTTCGCTTTGATGCTAACGGAGCCACAGGACCAG
GGAGCCGGGACCCCGCCCAGGTGAAGCTGCTGCCTGGAAGGCACATGCTCTTCCCTCCTCTTGAGAGAGT
CCACTACTTACAGGTCCCCCTATCCCGGCGTCTGTCCCATGATGAGACAAACATCTTCTCTACCCCTCGG
GAACCAGGCTCCTTCCTGCACAAGTGGTCATCCTCTGATGACATCCGGGTCCTCCCAGCCCAGAGCCGGG
CCCTCGGGGGTCCTCCTGAGTACCTGGGACAAAGACACAGGTTGGAGGACGAGGAGGACGAGGAAGAGGC
TGAAGGTGGGGGGCTGGCCAGCCTTCGCCAATTCTTGGAGAGTGGGGTTCTGGGGTCAGGTGGGGGACCC
CCACGGGGTCCTGGCTTCTTCCGGGAGGAGATCACCACCTTCATCGATGAGACACCTCTGCCTTCTCCGA
CTGCCTCACCAGGGCACTCTCCTCGTCGGCCCCGGCCACTGGGCCTCTCACCCCGCCGACTCTCCCTTGG
GTCCCCTGAGAGCAGAGCCGTTGGACTTCCTTTGGGACTAAGCGCAGGGAGACGCTGCTCCCTGACGGGG
GGTGAAGAAAGTGCAAGGGCTTGGGGAGGATCCTGGGGCCCAGGCAACCCCATCTTTCCCCAGCTGACCC
TG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG201764 representing NM_014449
Red=Cloning site Green=Tags(s)

MLSTGVVSFFSLKSDSAPPWMVLAVLWCSMAQTLLLPSFIWSCERYRADVRTVWEQCVAIMSEEDGDDDG
GCDDYAEGRVCKVRFDANGATGPGSRDPAQVKLLPGRHMLFPPLERVHYLQVPLSRRLSHDETNIFSTPR
EPGSFLHKWSSSDDIRVLPAQSRALGGPPEYLGQRHRLEDEEDEEEAEGGGLASLRQFLESGVLGSGGGP
PRGPGFFREEITTFIDETPLPSPTASPGHSPRRPRPLGLSPRRLSLGSPESRAVGLPLGLSAGRRCSLTG
GEESARAWGGSWGPGNPIFPQLTL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_014449
ORF Size 912 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_014449.2
RefSeq Size 1514 bp
RefSeq ORF 915 bp
Locus ID 27239
UniProt ID Q16538
Cytogenetics 12p13.31
Protein Families Druggable Genome, GPCR, Transmembrane
Summary This gene was identified upon genomic analysis of a gene-dense region at human chromosome 12p13. It appears to be mainly expressed in the brain; however, its function is not known. Alternatively spliced transcript variants encoding different isoforms have been identified. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_014449" in other vectors (4)
SKU Description Size Price
RC201764 GPR162 (Myc-DDK-tagged)-Human G protein-coupled receptor 162 (GPR162), transcript variant A-1 10 ug
$300.00
RC201764L3 Lenti ORF clone of Human G protein-coupled receptor 162 (GPR162), transcript variant A-1, Myc-DDK-tagged 10 ug
$600.00
RC201764L4 Lenti ORF clone of Human G protein-coupled receptor 162 (GPR162), transcript variant A-1, mGFP tagged 10 ug
$600.00
SC304111 GPR162 (untagged)-Human G protein-coupled receptor 162 (GPR162), transcript variant A-1 10 ug
$300.00

Plasmid Map for RG201764

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG201764
Circular map for RG201764

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