MPP1 (NM_002436) Human Tagged ORF Clone

SKU RG201754

MPP1 (tGFP-tagged) - Human membrane protein, palmitoylated 1, 55kDa (MPP1), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: MPP1
• Synonyms: AAG12; DXS552E; EMP55; MRG1; PEMP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG201754 representing NM_002436; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACCCTCAAGGCGAGCGAGGGCGAGAGTGGGGGCAGCATGCACACGGCGCTCTCCGACCTCTACCTGG; AGCATTTGCTGCAGAAGCGTAGTCGGCCAGAGGCTGTATCGCATCCATTGAATACTGTGACCGAGGACAT; GTACACCAACGGGTCTCCTGCCCCAGGTAGCCCTGCCCAGGTCAAGGGACAGGAGGTGCGGAAAGTGCGA; CTCATACAGTTTGAGAAGGTCACAGAAGAGCCCATGGGAATCACGCTGAAGCTGAATGAAAAACAGTCCT; GTACGGTGGCCAGAATTCTTCATGGTGGCATGATCCATAGACAAGGCTCCCTTCACGTGGGGGATGAGAT; CCTAGAAATCAATGGCACAAATGTGACAAATCATTCAGTGGATCAGCTGCAGAAGGCGATGAAAGAAACC; AAAGGAATGATCTCATTAAAAGTAATTCCCAACCAGCAAAGCCGTCTTCCTGCACTACAGATGTTCATGA; GAGCGCAGTTTGACTATGATCCCAAAAAGGACAATCTGATCCCTTGCAAGGAGGCGGGACTGAAGTTTGC; TACTGGGGACATTATCCAGATTATCAACAAGGATGACAGCAATTGGTGGCAGGGACGGGTGGAAGGCTCC; TCCAAGGAGTCAGCAGGATTGATCCCTTCCCCTGAGCTGCAGGAATGGCGAGTGGCAAGTATGGCTCAGT; CAGCTCCTAGCGAAGCCCCGAGCTGCAGTCCCTTTGGGAAGAAGAAGAAGTACAAAGACAAATATCTGGC; CAAGCACAGCTCGATTTTTGATCAGTTGGATGTTGTTTCCTACGAGGAAGTCGTTCGGCTCCCTGCATTC; AAGAGGAAGACCCTGGTGCTGATCGGAGCCAGTGGGGTGGGTCGCAGCCACATTAAGAATGCCCTGCTCA; GCCAGAATCCGGAGAAGTTTGTGTACCCTGTCCCATATACAACACGGCCGCCAAGGAAGAGTGAGGAAGA; TGGGAAGGAGTACCACTTTATCTCAACGGAGGAGATGACGAGGAACATCTCTGCCAATGAGTTCTTGGAG; TTTGGCAGCTACCAAGGCAACATGTTTGGCACCAAATTTGAAACAGTGCACCAGATCCATAAGCAGAACA; AGATTGCCATCCTTGACATTGAGCCCCAGACCCTGAAAATTGTTCGGACAGCAGAACTTTCGCCTTTCAT; TGTGTTCATTGCACCTACTGACCAGGGCACTCAGACAGAAGCCCTGCAGCAGCTGCAGAAGGACTCTGAG; GCCATCCGCAGCCAGTACGCTCACTACTTTGACCTCTCACTGGTCAATAATGGTGTTGATGAAACCCTTA; AGAAATTACAAGAAGCCTTCGACCAAGCGTGCAGTTCTCCACAGTGGGTGCCTGTCTCCTGGGTTTAC; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG201754 representing NM_002436; Red=Cloning site Green=Tags(s); ; MTLKASEGESGGSMHTALSDLYLEHLLQKRSRPEAVSHPLNTVTEDMYTNGSPAPGSPAQVKGQEVRKVR; LIQFEKVTEEPMGITLKLNEKQSCTVARILHGGMIHRQGSLHVGDEILEINGTNVTNHSVDQLQKAMKET; KGMISLKVIPNQQSRLPALQMFMRAQFDYDPKKDNLIPCKEAGLKFATGDIIQIINKDDSNWWQGRVEGS; SKESAGLIPSPELQEWRVASMAQSAPSEAPSCSPFGKKKKYKDKYLAKHSSIFDQLDVVSYEEVVRLPAF; KRKTLVLIGASGVGRSHIKNALLSQNPEKFVYPVPYTTRPPRKSEEDGKEYHFISTEEMTRNISANEFLE; FGSYQGNMFGTKFETVHQIHKQNKIAILDIEPQTLKIVRTAELSPFIVFIAPTDQGTQTEALQQLQKDSE; AIRSQYAHYFDLSLVNNGVDETLKKLQEAFDQACSSPQWVPVSWVY; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002436
• ORF Size: 1398 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002436.4
• RefSeq Size: 2001 bp
• RefSeq ORF: 1401 bp
• Locus ID: 4354
• UniProt ID: Q00013
• Cytogenetics: Xq28
• Domains: Guanylate_kin, GuKc, PDZ, SH3
• Protein Families: Druggable Genome
• Summary: This gene encodes the prototype of the membrane-associated guanylate kinase (MAGUK) family proteins. MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intercellular junctions. The encoded protein is an extensively palmitoylated membrane phosphoprotein containing a PDZ domain, a Src homology 3 (SH3) motif, and a guanylate kinase domain. This gene product interacts with various cytoskeletal proteins and cell junctional proteins in different tissue and cell types, and may be involved in the regulation of cell shape, hair cell development, neural patterning of the retina, and apico-basal polarity and tumor suppression pathways in non-erythroid cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]