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EI24 (NM_004879) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG201737
EI24 (tGFP-tagged) - Human etoposide induced 2.4 mRNA (EI24), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $657.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol EI24
Synonyms EPG4; PIG8; TP53I8
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG201737 representing NM_004879
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGACAGTGTCAAAACCTTTCTCCAGGACCTTGCCAGAGGAATCAAAGACTCCATCTGGGGTATTT
GTACCATCTCAAAGCTAGATGCTCGAATCCAGCAAAAGAGAGAGGAGCAGCGTCGAAGAAGGGCAAGTAG
TGTCTTGGCACAGAGAAGAGCCCAGAGTATAGAGCGGAAGCAAGAGAGTGAGCCACGTATTGTTAGTAGA
ATTTTCCAGTGTTGTGCTTGGAATGGTGGAGTGTTCTGGTTCAGTCTCCTCTTGTTTTATCGAGTATTTA
TTCCTGTGCTTCAGTCGGTAACAGCCCGAATTATCGGTGACCCATCACTACATGGAGATGTTTGGTCGTG
GCTGGAATTCTTCCTCACGTCAATTTTCAGTGCTCTTTGGGTGCTCCCCTTGTTTGTGCTTAGCAAAGTG
GTGAATGCCATTTGGTTTCAGGATATAGCTGACCTGGCATTTGAGGTATCAGGGAGGAAGCCTCACCCAT
TCCCTAGTGTCAGCAAAATAATTGCTGACATGCTCTTCAACCTTTTGCTGCAGGCTCTTTTCCTCATTCA
GGGAATGTTTGTGAGTCTCTTTCCCATCCATCTTGTCGGTCAGCTGGTTAGTCTCCTGCATATGTCCCTT
CTCTACTCACTGTACTGCTTTGAATATCGTTGGTTCAATAAAGGAATTGAAATGCACCAGCGGTTGTCTA
ACATAGAAAGGAATTGGCCTTACTACTTTGGGTTTGGTTTGCCCTTGGCTTTTCTCACAGCAATGCAGTC
CTCATATATTATCAGTGGCTGCCTTTTCTCTATCCTCTTTCCTTTATTCATTATCAGCGCCAATGAAGCA
AAGACCCCTGGCAAAGCATATCTCTTCCAGTTGCGCCTCTTCTCCTTGGTGGTCTTCTTAAGCAACAGAC
TCTTCCACAAGACAGTCTACCTGCAGTCGGCCCTGAGCAGCTCTACTTCTGCAGAGAAGTTCCCTTCACC
GCATCCGTCGCCTGCCAAACTGAAGGCTACTGCAGGTCAC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG201737 representing NM_004879
Red=Cloning site Green=Tags(s)

MADSVKTFLQDLARGIKDSIWGICTISKLDARIQQKREEQRRRRASSVLAQRRAQSIERKQESEPRIVSR
IFQCCAWNGGVFWFSLLLFYRVFIPVLQSVTARIIGDPSLHGDVWSWLEFFLTSIFSALWVLPLFVLSKV
VNAIWFQDIADLAFEVSGRKPHPFPSVSKIIADMLFNLLLQALFLIQGMFVSLFPIHLVGQLVSLLHMSL
LYSLYCFEYRWFNKGIEMHQRLSNIERNWPYYFGFGLPLAFLTAMQSSYIISGCLFSILFPLFIISANEA
KTPGKAYLFQLRLFSLVVFLSNRLFHKTVYLQSALSSSTSAEKFPSPHPSPAKLKATAGH

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004879
ORF Size 1020 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004879.5
RefSeq Size 2279 bp
RefSeq ORF 1023 bp
Locus ID 9538
UniProt ID O14681
Cytogenetics 11q24.2
Protein Families Druggable Genome, Transmembrane
Protein Pathways p53 signaling pathway
Summary This gene encodes a putative tumor suppressor and has higher expression in p53-expressing cells than in control cells and is an immediate-early induction target of p53-mediated apoptosis. The encoded protein may suppress cell growth by inducing apoptotic cell death through the caspase 9 and mitochondrial pathways. This gene is located on human chromosome 11q24, a region frequently altered in cancers. Alternative splicing results in multiple transcript variants. Pseudogenes of this gene have been defined on chromosomes 1, 3, 7, and 8. provided by RefSeq, Feb 2014
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"NM_004879" in other vectors (6)
SKU Description Size Price
RC201737 EI24 (Myc-DDK-tagged)-Human etoposide induced 2.4 mRNA (EI24), transcript variant 1 10 ug
$457.00
RC201737L1 Lenti ORF clone of Human etoposide induced 2.4 mRNA (EI24), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC201737L2 Lenti ORF clone of Human etoposide induced 2.4 mRNA (EI24), transcript variant 1, mGFP tagged 10 ug
$757.00
RC201737L3 Lenti ORF clone of Human etoposide induced 2.4 mRNA (EI24), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC201737L4 Lenti ORF clone of Human etoposide induced 2.4 mRNA (EI24), transcript variant 1, mGFP tagged 10 ug
$757.00
SC117074 EI24 (untagged)-Human etoposide induced 2.4 mRNA (EI24), transcript variant 1 10 ug
$457.00

Plasmid Map for RG201737

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG201737
Circular map for RG201737

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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