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NET1 (NM_005863) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG201682
NET1 (tGFP-tagged) - Human neuroepithelial cell transforming 1 (NET1), transcript variant 2
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $705.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NET1
Synonyms ARHGEF8; NET1A
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG201682 representing NM_005863
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGGCACATGATGAGACTGGAGGTCTCCTACCTATTAAAAGGACCATACGAGTCCTAGATGTCAATA
ACCAGTCCTTCAGAGAACAAGAGGAGCCAAGCAATAAAAGAGTTCGACCTCTGGCTCGTGTCACGTCCTT
GGCAAATTTAATCTCTCCTGTAAGAAATGGAGCTGTCAGACGTTTTGGTCAAACAATACAGTCATTTACC
CTTCGTGGTGACCACAGATCCCCAGCCTCTGCCCAGAAGTTTTCTAGCAGGTCAACAGTCCCAACACCCG
CCAAGAGAAGGAGCAGTGCACTGTGGTCAGAGATGCTGGACATCACCATGAAGGAGTCTCTCACCACCAG
GGAGATCAGACGGCAGGAGGCAATATATGAAATGTCCCGAGGTGAACAGGATTTAATTGAGGATCTCAAA
CTTGCAAGAAAGGCCTACCATGACCCCATGTTAAAGTTGTCCATCATGTCAGAAGAGGAACTCACACATA
TATTTGGTGATCTGGACTCTTACATACCTCTGCATGAAGATTTGTTGACAAGAATAGGAGAAGCAACCAA
GCCTGATGGAACAGTGGAGCAGATTGGTCACATTCTCGTGAGCTGGTTACCGCGCTTGAATGCCTACAGA
GGTTACTGTAGTAACCAGCTGGCAGCCAAAGCTCTTCTTGATCAAAAGAAACAGGATCCAAGAGTCCAAG
ACTTCCTCCAGCGATGTCTCGAGTCTCCCTTCAGTCGAAAACTAGATCTTTGGAGTTTCCTAGATATCCC
TCGAAGTCGCCTAGTCAAATACCCTTTACTGTTAAAAGAAATTCTTAAACACACTCCAAAAGAGCACCCT
GATGTTCAGCTTCTGGAGGATGCTATATTGATAATACAGGGAGTCCTCTCTGATATCAACTTGAAGAAAG
GTGAATCCGAGTGCCAGTATTACATCGACAAGCTGGAGTACCTGGATGAAAAGCAGAGGGACCCCAGAAT
CGAAGCGAGCAAAGTGCTGCTGTGCCATGGGGAGCTGCGGAGCAAGAGTGGACATAAACTTTACATTTTC
CTGTTTCAAGACATCTTGGTTCTGACTCGGCCCGTCACACGGAACGAACGGCACTCTTACCAGGTTTACC
GGCAGCCAATCCCAGTCCAAGAGCTAGTCCTAGAAGACCTGCAGGATGGAGATGTGAGAATGGGAGGCTC
CTTTCGAGGAGCTTTCAGTAACTCAGAGAAAGCTAAAAATATCTTTAGAATTCGCTTCCATGACCCCTCT
CCAGCCCAGTCTCACACTCTGCAAGCCAATGACGTGTTCCACAAGCAGCAGTGGTTCAACTGTATTCGAG
CGGCCATTGCCCCCTTCCAGTCGGCAGGCAGTCCACCTGAGCTGCAGGGCCTGCCGGAGCTGCACGAAGA
GTGTGAGGGGAACCACCCCTCTGCGAGGAAACTCACAGCCCAGAGGAGGGCATCCACAGTTTCCAGTGTT
ACTCAGGTAGAAGTTGATGAAAACGCTTACAGATGTGGCTCTGGCATGCAGATGGCAGAGGACAGCAAGA
GCTTAAAGACACACCAGACACAGCCCGGCATCCGAAGAGCGAGGGACAAAGCCCTTTCTGGTGGCAAACG
GAAAGAGACTTTGGTG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG201682 representing NM_005863
Red=Cloning site Green=Tags(s)

MVAHDETGGLLPIKRTIRVLDVNNQSFREQEEPSNKRVRPLARVTSLANLISPVRNGAVRRFGQTIQSFT
LRGDHRSPASAQKFSSRSTVPTPAKRRSSALWSEMLDITMKESLTTREIRRQEAIYEMSRGEQDLIEDLK
LARKAYHDPMLKLSIMSEEELTHIFGDLDSYIPLHEDLLTRIGEATKPDGTVEQIGHILVSWLPRLNAYR
GYCSNQLAAKALLDQKKQDPRVQDFLQRCLESPFSRKLDLWSFLDIPRSRLVKYPLLLKEILKHTPKEHP
DVQLLEDAILIIQGVLSDINLKKGESECQYYIDKLEYLDEKQRDPRIEASKVLLCHGELRSKSGHKLYIF
LFQDILVLTRPVTRNERHSYQVYRQPIPVQELVLEDLQDGDVRMGGSFRGAFSNSEKAKNIFRIRFHDPS
PAQSHTLQANDVFHKQQWFNCIRAAIAPFQSAGSPPELQGLPELHEECEGNHPSARKLTAQRRASTVSSV
TQVEVDENAYRCGSGMQMAEDSKSLKTHQTQPGIRRARDKALSGGKRKETLV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005863
ORF Size 1626 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005863.4
RefSeq Size 3297 bp
RefSeq ORF 1629 bp
Locus ID 10276
UniProt ID Q7Z628
Cytogenetics 10p15.1
Domains PH, RhoGEF
Protein Families Druggable Genome
Summary This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms. provided by RefSeq, Jul 2012
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Documents
Resources
"NM_005863" in other vectors (6)
SKU Description Size Price
RC201682 NET1 (Myc-DDK-tagged)-Human neuroepithelial cell transforming 1 (NET1), transcript variant 2 10 ug
$505.00
RC201682L1 Lenti ORF clone of Human neuroepithelial cell transforming 1 (NET1), transcript variant 2, Myc-DDK-tagged 10 ug
$805.00
RC201682L2 Lenti ORF clone of Human neuroepithelial cell transforming 1 (NET1), transcript variant 2, mGFP tagged 10 ug
$805.00
RC201682L3 Lenti ORF clone of Human neuroepithelial cell transforming 1 (NET1), transcript variant 2, Myc-DDK-tagged 10 ug
$805.00
RC201682L4 Lenti ORF clone of Human neuroepithelial cell transforming 1 (NET1), transcript variant 2, mGFP tagged 10 ug
$805.00
SC116508 NET1 (untagged)-Human neuroepithelial cell transforming 1 (NET1), transcript variant 2 10 ug
$505.00

Plasmid Map for RG201682

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG201682
Circular map for RG201682

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