TRA2B (NM_004593) Human Tagged ORF Clone

  • TrueORF®
SKU
RG201542
TRA2B (tGFP-tagged) - Human transformer 2 beta homolog (Drosophila) (TRA2B)
$489.00 $500.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TRA2B
Synonyms Htra2-beta; PPP1R156; SFRS10; SRFS10; TRA2-BETA; TRAN2B
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG201542 representing NM_004593
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGACAGCGGCGAGCAGAACTACGGCGAGCGGGAATCCCGTTCTGCTTCCAGAAGTGGAAGTGCTC
ACGGATCGGGGAAATCTGCAAGGCATACCCCTGCAAGGTCTCGCTCCAAGGAAGATTCCAGGCGTTCCAG
ATCAAAGTCCAGGTCCCGATCTGAATCTAGGTCTAGATCCAGAAGAAGCTCCCGAAGGCATTATACCCGG
TCACGGTCTCGCTCCCGCTCCCATAGACGATCACGTAGCAGGTCTTACAGTCGAGATTATCGTAGACGGC
ACAGCCACAGCCATTCTCCCATGTCTACTCGCAGGCGTCATGTTGGGAATCGGGCAAATCCTGATCCTAA
CTGTTGTCTTGGAGTATTTGGGCTGAGCTTGTACACCACAGAAAGAGATCTAAGAGAAGTGTTCTCTAAA
TATGGTCCCATTGCCGATGTGTCTATTGTATATGACCAGCAGTCTAGGCGTTCAAGAGGATTTGCCTTTG
TATATTTTGAAAATGTAGATGATGCCAAGGAAGCTAAAGAACGTGCCAATGGAATGGAGCTTGATGGGCG
TAGGATCAGAGTTGATTTCTCTATAACAAAAAGACCACATACGCCAACACCAGGAATTTACATGGGGAGA
CCTACCTATGGCAGCTCTCGCCGTCGGGATTACTATGACAGAGGATATGATCGGGGCTATGATGATCGGG
ACTACTATAGCAGATCATACAGAGGAGGAGGTGGAGGAGGAGGAGGATGGAGAGCTGCCCAAGACAGGGA
TCAGATTTATAGAAGGCGGTCACCTTCTCCTTACTATAGTCGTGGAGGATACAGATCACGTTCCAGATCT
CGATCATACTCACCTCGTCGCTAT


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG201542 representing NM_004593
Red=Cloning site Green=Tags(s)

MSDSGEQNYGERESRSASRSGSAHGSGKSARHTPARSRSKEDSRRSRSKSRSRSESRSRSRRSSRRHYTR
SRSRSRSHRRSRSRSYSRDYRRRHSHSHSPMSTRRRHVGNRANPDPNCCLGVFGLSLYTTERDLREVFSK
YGPIADVSIVYDQQSRRSRGFAFVYFENVDDAKEAKERANGMELDGRRIRVDFSITKRPHTPTPGIYMGR
PTYGSSRRRDYYDRGYDRGYDDRDYYSRSYRGGGGGGGGWRAAQDRDQIYRRRSPSPYYSRGGYRSRSRS
RSYSPRRY

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_004593
ORF Size 864 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_004593.3
RefSeq Size 1972 bp
RefSeq ORF 867 bp
Locus ID 6434
UniProt ID P62995
Cytogenetics 3q27.2
Domains RRM
Protein Families Druggable Genome
Protein Pathways Spliceosome
Summary This gene encodes a nuclear protein which functions as sequence-specific serine/arginine splicing factor which plays a role in mRNA processing, splicing patterns, and gene expression. Alternative splicing results in multiple transcript variants. provided by RefSeq, Aug 2011
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Documents
Resources
"NM_004593" in other vectors (7)
SKU Description Size Price
RC201542 TRA2B (Myc-DDK-tagged)-Human transformer 2 beta homolog (Drosophila) (TRA2B) 10 ug
$300.00
RC201542L1 Lenti ORF clone of Human transformer 2 beta homolog (Drosophila) (TRA2B), Myc-DDK-tagged 10 ug
$600.00
RC201542L2 Lenti ORF clone of Human transformer 2 beta homolog (Drosophila) (TRA2B), mGFP tagged 10 ug
$600.00
RC201542L3 Lenti ORF clone of Human transformer 2 beta homolog (Drosophila) (TRA2B), Myc-DDK-tagged 10 ug
$600.00
RC201542L4 Lenti ORF clone of Human transformer 2 beta homolog (Drosophila) (TRA2B), mGFP tagged 10 ug
$600.00
SC108518 TRA2B (untagged)-Human transformer 2 beta homolog (Drosophila) (TRA2B) 10 ug
$300.00
SC322178 TRA2B (untagged)-Human transformer 2 beta homolog (Drosophila) (TRA2B) 10 ug
$300.00

Plasmid Map for RG201542

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Cloning scheme for RG201542

Circular map for RG201542

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