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SRSF6 (NM_006275) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG201541
SRSF6 (tGFP-tagged) - Human serine/arginine-rich splicing factor 6 (SRSF6), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $657.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SRSF6
Synonyms B52; HEL-S-91; SFRS6; SRP55
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG201541 representing NM_006275
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGGCGCGCCC

ATGCCGCGCGTCTACATAGGACGCCTGAGCTACAACGTCCGGGAGAAGGACATCCAGCGCTTTTTCAGTG
GCTATGGCCGCCTCCTCGAAGTAGACCTCAAAAATGGGTACGGCTTCGTGGAGTTCGAGGACTCCCGCGA
CGCCGACGACGCCGTTTACGAGCTGAACGGCAAGGAGCTCTGCGGCGAGCGCGTGATCGTAGAGCACGCC
CGGGGCCCGCGTCGCGATCGCGACGGCTACAGCTACGGAAGCCGCAGTGGTGGAGGTGGATACAGCAGTC
GGAGAACATCTGGCAGAGACAAATACGGACCACCTGTTCGTACAGAATACAGGCTTATTGTAGAAAATCT
TTCTAGTCGGTGCAGTTGGCAAGATTTAAAGGATTTTATGCGACAAGCAGGTGAAGTAACCTATGCGGAT
GCCCACAAGGAACGAACAAATGAGGGTGTAATTGAGTTTCGCTCCTACTCTGACATGAAGCGTGCTTTGG
ACAAACTGGATGGCACAGAAATAAATGGCAGAAATATTAGGCTTATTGAAGATAAGCCACGCACAAGCCA
TAGGCGATCTTACTCTGGAAGCAGATCCAGGTCTCGATCTAGAAGACGGTCACGAAGTAGGAGTCGCAGG
AGCAGCCGCAGTAGATCTCGAAGTATCTCAAAAAGTCGCTCCCGTTCCAGGTCGCGGAGCAAAGGTCGAT
CACGTTCTCGATCAAAAGGCAGGAAATCTAGATCAAAGAGCAAATCTAAGCCCAAGTCTGATCGGGGCTC
CCATTCACATTCTCGAAGCAGATCTAAGGATGAGTATGAGAAATCTCGAAGCAGGTCTCGGTCCCGATCC
CCCAAAGAAAATGGAAAGGGTGATATAAAGTCAAAATCCAGATCAAGGAGCCAGTCCCGTTCCAATTCGC
CGCTACCTGTTCCACCCTCAAAGGCCCGTTCTGTGTCCCCTCCACCAAAAAGAGCTACTTCAAGATCCCG
TTCTAGATCTCGCTCAAAGTCAAGATCAAGGTCCAGGTCGAGTTCCAGAGAT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG201541 representing NM_006275
Red=Cloning site Green=Tags(s)

MPRVYIGRLSYNVREKDIQRFFSGYGRLLEVDLKNGYGFVEFEDSRDADDAVYELNGKELCGERVIVEHA
RGPRRDRDGYSYGSRSGGGGYSSRRTSGRDKYGPPVRTEYRLIVENLSSRCSWQDLKDFMRQAGEVTYAD
AHKERTNEGVIEFRSYSDMKRALDKLDGTEINGRNIRLIEDKPRTSHRRSYSGSRSRSRSRRRSRSRSRR
SSRSRSRSISKSRSRSRSRSKGRSRSRSKGRKSRSKSKSKPKSDRGSHSHSRSRSKDEYEKSRSRSRSRS
PKENGKGDIKSKSRSRSQSRSNSPLPVPPSKARSVSPPPKRATSRSRSRSRSKSRSRSRSSSRD

TRTRPLE - GFP Tag - V
Restriction Sites AscI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006275
ORF Size 1032 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006275.4, NP_006266.2
RefSeq Size 3738 bp
RefSeq ORF 1035 bp
Locus ID 6431
UniProt ID Q13247
Cytogenetics 20q13.11
Domains RRM
Protein Pathways Spliceosome
Summary The protein encoded by this gene is involved in mRNA splicing and may play a role in the determination of alternative splicing. The encoded nuclear protein belongs to the splicing factor SR family and has been shown to bind with and modulate another member of the family, SFRS12. Alternative splicing results in multiple transcript variants. In addition, two pseudogenes, one on chromosome 17 and the other on the X chromosome, have been found for this gene.provided by RefSeq, Sep 2010
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"NM_006275" in other vectors (6)
SKU Description Size Price
RC201541 SRSF6 (Myc-DDK-tagged)-Human serine/arginine-rich splicing factor 6 (SRSF6), transcript variant 1 10 ug
$457.00
RC201541L1 Lenti ORF clone of Human serine/arginine-rich splicing factor 6 (SRSF6), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC201541L2 Lenti ORF clone of Human serine/arginine-rich splicing factor 6 (SRSF6), transcript variant 1, mGFP tagged 10 ug
$757.00
RC201541L3 Lenti ORF clone of Human serine/arginine-rich splicing factor 6 (SRSF6), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC201541L4 Lenti ORF clone of Human serine/arginine-rich splicing factor 6 (SRSF6), transcript variant 1, mGFP tagged 10 ug
$757.00
SC125351 SRSF6 (untagged)-Human serine/arginine-rich splicing factor 6 (SRSF6), transcript variant 1 10 ug
$457.00

Plasmid Map for RG201541

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG201541
Circular map for RG201541

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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