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ISCA1 (NM_030940) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG201335
ISCA1 (tGFP-tagged) - Human iron-sulfur cluster assembly 1 homolog (S. cerevisiae) (ISCA1)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $489.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ISCA1
Synonyms HBLD2; hIscA; ISA1; MMDS5
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG201335 representing NM_030940
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCGGCTTCCTTAGTCCGGGCAACTGTCCGGGCTGTGAGCAAGAGGAAGCTGCAGCCCACCCGGGCAG
CCCTCACCCTGACACCTTCAGCAGTAAACAAGATAAAACAACTTCTTAAAGATAAGCCTGAGCATGTAGG
TGTAAAAGTTGGTGTCCGAACCAGGGGCTGTAATGGCCTTTCTTATACTCTAGAATATACAAAGACAAAA
GGAGATTCTGATGAAGAAGTTATTCAAGATGGAGTCAGAGTATTCATCGAAAAGAAAGCACAGCTAACAC
TTTTAGGAACAGAAATGGACTATGTTGAAGACAAATTATCCAGTGAGTTTGTGTTCAATAACCCAAACAT
CAAAGGGACTTGTGGCTGTGGAGAAAGCTTTAATATT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG201335 representing NM_030940
Red=Cloning site Green=Tags(s)

MSASLVRATVRAVSKRKLQPTRAALTLTPSAVNKIKQLLKDKPEHVGVKVGVRTRGCNGLSYTLEYTKTK
GDSDEEVIQDGVRVFIEKKAQLTLLGTEMDYVEDKLSSEFVFNNPNIKGTCGCGESFNI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_030940
ORF Size 387 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030940.4
RefSeq Size 2012 bp
RefSeq ORF 390 bp
Locus ID 81689
UniProt ID Q9BUE6
Cytogenetics 9q21.33
Domains HesB-like
Summary ISCA1 is a mitochondrial protein involved in the biogenesis and assembly of iron-sulfur clusters, which play a role in electron-transfer reactions (Cozar-Castellano et al., 2004 PubMed 15262227).supplied by OMIM, Mar 2008
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"NM_030940" in other vectors (4)
SKU Description Size Price
RC201335 ISCA1 (Myc-DDK-tagged)-Human iron-sulfur cluster assembly 1 homolog (S. cerevisiae) (ISCA1) 10 ug
$150.00
RC201335L3 Lenti ORF clone of Human iron-sulfur cluster assembly 1 homolog (S. cerevisiae) (ISCA1), Myc-DDK-tagged 10 ug
$450.00
RC201335L4 Lenti ORF clone of Human iron-sulfur cluster assembly 1 homolog (S. cerevisiae) (ISCA1), mGFP tagged 10 ug
$450.00
SC127008 ISCA1 (untagged)-Human iron-sulfur cluster assembly 1 homolog (S. cerevisiae) (ISCA1) 10 ug
$150.00

Plasmid Map for RG201335

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG201335
Circular map for RG201335

file-alt Citations

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