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DCP1A (NM_018403) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG201330
DCP1A (tGFP-tagged) - Human DCP1 decapping enzyme homolog A (S. cerevisiae) (DCP1A)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$489.00 $742.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DCP1A
Synonyms HSA275986; Nbla00360; SMAD4IP1; SMIF
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG201330 representing NM_018403
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGCGCTGAGTCGAGCTGGGCAGGAGATGAGCCTAGCGGCCCTGAAGCAACACGACCCCTATATCA
CCAGCATCGCAGACCTCACGGGCCAGGTCGCTCTGTACACCTTCTGCCCCAAGGCCAACCAGTGGGAGAA
GACTGATATAGAAGGGACCTTATTCGTATATCGAAGGTCAGCTTCCCCTTACCATGGTTTTACCATTGTG
AATCGACTAAATATGCACAATCTAGTTGAACCAGTGAATAAAGATTTGGAATTTCAGCTCCATGAACCAT
TTCTTCTGTATAGAAATGCAAGCTTGTCGATATATAGTATCTGGTTTTATGACAAGAATGACTGTCACCG
CATAGCAAAACTCATGGCTGATGTGGTAGAAGAGGAGACACGGCGATCCCAGCAAGCTGCTCGGGACAAA
CAGAGTCCCAGCCAGGCCAATGGCTGCAGCGACCACAGGCCCATCGACATCCTGGAGATGCTGAGCAGAG
CCAAGGATGAGTATGAGAGGAATCAGATGGGTGACTCAAATATCTCCAGCCCTGGGTTACAGCCAAGCAC
TCAGCTCTCCAATCTGGGAAGCACCGAGACTCTAGAAGAAATGCCCTCCGGGTCACAGGATAAGTCTGCT
CCATCTGGACACAAGCATCTGACGGTAGAAGAGTTATTTGGAACCTCTTTGCCAAAGGAACAACCAGCAG
TTGTGGGTCTGGATTCAGAAGAAATGGAGAGGTTGCCAGGAGATGCCTCCCAGAAAGAGCCCAATTCATT
CCTACCATTTCCCTTTGAGCAGTTAGGAGGAGCCCCTCAATCAGAAACCCTGGGTGTCCCTTCTGCTGCC
CACCATTCAGTCCAGCCTGAAATCACCACCCCGGTGCTAATCACTCCAGCCTCCATCACACAGTCCAATG
AAAAGCATGCTCCAACCTACACAATCCCGTTGAGCCCTGTTCTCAGTCCCACTCTGCCAGCTGAAGCTCC
TACTGCACAGGTTCCCCCCAGCTTACCTCGAAACAGCACCATGATGCAGGCAGTGAAGACCACGCCTAGA
CAGAGGTCTCCACTCCTGAACCAGCCAGTCCCTGAGCTAAGCCATGCCAGTCTGATTGCCAACCAGAGCC
CCTTCAGGGCCCCATTGAACGTGACGAACACAGCTGGCACATCCCTCCCAAGCGTTGATCTTCTCCAGAA
ACTCAGGTTGACCCCACAGCATGACCAAATACAGACACAACCACTTGGGAAAGGTGCAATGGTAGCCAGC
TTTTCTCCGGCAGCTGGTCAGCTAGCCACACCTGAGAGCTTCATAGAGCCTCCCTCTAAGACAGCAGCAG
CAAGAGTGGCGGCCTCAGCCTCCCTGAGCAACATGGTGCTTGCTCCCCTTCAGTCTATGCAGCAGAACCA
GGATCCTGAAGTATTTGTGCAGCCTAAGGTGTTATCCAGTGCCATCCCGGTTGCAGGCGCCCCACTGGTT
ACTGCAACGACCACTGCAGTGTCTTCAGTCCTGCTGGCCCCAAGTGTTTTCCAGCAGACAGTTACAAGAT
CTTCGGACCTTGAGAGGAAAGCCAGCTCCCCTTCTCCTCTAACTATTGGAACGCCAGAAAGTCAGAGAAA
GCCTTCCATTATTCTCAGCAAGTCTCAGCTCCAGGATACATTAATACATCTAATAAAGAATGATTCCAGC
TTCCTCAGTACACTTCATGAAGTCTACTTGCAGGTTCTGACCAAGAACAAAGACAACCACAACCTA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG201330 representing NM_018403
Red=Cloning site Green=Tags(s)

MEALSRAGQEMSLAALKQHDPYITSIADLTGQVALYTFCPKANQWEKTDIEGTLFVYRRSASPYHGFTIV
NRLNMHNLVEPVNKDLEFQLHEPFLLYRNASLSIYSIWFYDKNDCHRIAKLMADVVEEETRRSQQAARDK
QSPSQANGCSDHRPIDILEMLSRAKDEYERNQMGDSNISSPGLQPSTQLSNLGSTETLEEMPSGSQDKSA
PSGHKHLTVEELFGTSLPKEQPAVVGLDSEEMERLPGDASQKEPNSFLPFPFEQLGGAPQSETLGVPSAA
HHSVQPEITTPVLITPASITQSNEKHAPTYTIPLSPVLSPTLPAEAPTAQVPPSLPRNSTMMQAVKTTPR
QRSPLLNQPVPELSHASLIANQSPFRAPLNVTNTAGTSLPSVDLLQKLRLTPQHDQIQTQPLGKGAMVAS
FSPAAGQLATPESFIEPPSKTAAARVAASASLSNMVLAPLQSMQQNQDPEVFVQPKVLSSAIPVAGAPLV
TATTTAVSSVLLAPSVFQQTVTRSSDLERKASSPSPLTIGTPESQRKPSIILSKSQLQDTLIHLIKNDSS
FLSTLHEVYLQVLTKNKDNHNL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018403
ORF Size 1746 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018403.7
RefSeq Size 2054 bp
RefSeq ORF 1749 bp
Locus ID 55802
UniProt ID Q9NPI6
Cytogenetics 3p21.1
Protein Families Transcription Factors
Protein Pathways RNA degradation
Summary Decapping is a key step in general and regulated mRNA decay. The protein encoded by this gene is a decapping enzyme. This protein and another decapping enzyme form a decapping complex, which interacts with the nonsense-mediated decay factor hUpf1 and may be recruited to mRNAs containing premature termination codons. This protein also participates in the TGF-beta signaling pathway. Alternative splicing of this gene results in multiple transcript variants. provided by RefSeq, Feb 2014
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"NM_018403" in other vectors (7)
SKU Description Size Price
RC201330 DCP1A (Myc-DDK-tagged)-Human DCP1 decapping enzyme homolog A (S. cerevisiae) (DCP1A) 10 ug
$542.00
RC201330L1 Lenti ORF clone of Human DCP1 decapping enzyme homolog A (S. cerevisiae) (DCP1A), Myc-DDK-tagged 10 ug
$842.00
RC201330L2 Lenti ORF clone of Human DCP1 decapping enzyme homolog A (S. cerevisiae) (DCP1A), mGFP tagged 10 ug
$842.00
RC201330L3 Lenti ORF clone of Human DCP1 decapping enzyme homolog A (S. cerevisiae) (DCP1A), Myc-DDK-tagged 10 ug
$842.00
RC201330L4 Lenti ORF clone of Human DCP1 decapping enzyme homolog A (S. cerevisiae) (DCP1A), mGFP tagged 10 ug
$842.00
SC318001 DCP1A (untagged)-Human DCP1 decapping enzyme homolog A (S. cerevisiae) (DCP1A) 10 ug
$597.00
SC319488 DCP1A (untagged)-Human DCP1 decapping enzyme homolog A (S. cerevisiae) (DCP1A) 10 ug
$543.00

Plasmid Map for RG201330

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG201330
Circular map for RG201330

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