CCDC9 (NM_015603) Human Tagged ORF Clone

SKU
RG201107
CCDC9 (tGFP-tagged) - Human coiled-coil domain containing 9 (CCDC9)
  • TrueORF®
    TrueORF®

    Expression-ready ORF plasmid with C-terminal tag(s)

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$489.00 MSRP $694.00 MSRP $694.00
In Stock*
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CCDC9
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG201107 representing NM_015603
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCCACACTCGATTTGAAATCAAAGGAGGAGAAGGATGCTGAGTTGGACAAGAGGATCGAGGCTC
TTCGGCGGAAGAATGAGGCCCTCATCCGGCGCTACCAGGAGATTGAGGAAGACCGTAAGAAAGCTGAACT
TGAGGGAGTCGCAGTCACAGCTCCCCGAAAGGGCCGCTCAGTGGAGAAGGAGAACGTGGCAGTGGAGTCG
GAGAAGAACCTGGGTCCTTCCCGGAGGTCTCCTGGGACCCCTCGGCCCCCAGGGGCCAGCAAGGGGGGCC
GGACTCCTCCACAGCAGGGAGGCCGGGCCGGCATGGGCCGAGCATCGCGCAGCTGGGAGGGCAGCCCCGG
GGAGCAGCCTCGAGGAGGAGGAGCTGGGGGCCGTGGCCGGAGGGGCCGGGGCCGAGGTTCACCTCACCTC
TCTGGAGCTGGAGACACCTCAATCTCTGACCGTAAATCCAAGGAGTGGGAGGAGCGGCGCAGGCAGAACA
TTGAGAAGATGAATGAGGAGATGGAGAAGATCGCCGAGTATGAGCGCAACCAGCGGGAAGGGGTTCTTGA
ACCCAACCCAGTGCGGAACTTCCTGGACGACCCCCGGCGACGCAGCGGGCCCCTGGAGGAGTCTGAGCGG
GACCGCCGGGAGGAGAGCCGCCGGCACGGCCGCAACTGGGGGGGCCCCGACTTCGAGCGGGTGCGCTGTG
GCCTTGAGCACGAGCGGCAGGGCCGCCGAGCTGGCCTGGGCAGTGCTGGAGACATGACGTTGTCCATGAC
GGGCCGGGAGCGGTCGGAGTACCTGCGCTGGAAGCAGGAGAGGGAGAAGATCGACCAGGAGCGGCTGCAG
AGGCACCGCAAGCCCACTGGCCAGTGGAGGCGCGAGTGGGATGCCGAGAAGACCGATGGGATGTTCAAGG
ATGGCCCAGTCCCTGCCCATGAACCATCCCACCGCTATGATGACCAGGCCTGGGCCCGGCCCCCGAAGCC
CCCTACTTTTGGGGAGTTCCTGTCCCAGCACAAAGCTGAGGCCAGCAGCCGCAGAAGGAGAAAGAGCAGT
CGGCCCCAGGCCAAGGCAGCGCCCAGGGCCTACAGTGACCATGATGACCGCTGGGAGACAAAAGAAGGGG
CAGCATCCCCAGCCCCTGAGACTCCACAGCCTACTTCCCCCGAGACTTCCCCCAAGGAGACACCCATGCA
GCCACCCGAGATCCCAGCTCCTGCCCACCGGCCTCCTGAAGACGAGGGGGAAGAGAATGAGGGGGAAGAG
GATGAAGAATGGGAGGACATAAGTGAGGATGAGGAAGAGGAGGAGATCGAGGTGGAAGAAGGTGATGAGG
AGGAACCAGCCCAAGACCACCAAGCCCCAGAGGCTGCCCCCACCGGGATCCCCTGCAGTGAGCAGGCCCA
CGGAGTCCCCTTCAGTCCGGAGGAGCCCCTGCTGGAGCCCCAGGCCCCTGGCACGCCTTCCAGCCCTTTC
TCACCACCCAGCGGCCACCAGCCTGTGTCCGATTGGGGTGAAGAGGTGGAGCTGAATTCTCCCCGGACCA
CTCACCTGGCTGGCGCCCTCTCCCCGGGTGAGGCCTGGCCTTTTGAGAGTGTA


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG201107 representing NM_015603
Red=Cloning site Green=Tags(s)

MAATLDLKSKEEKDAELDKRIEALRRKNEALIRRYQEIEEDRKKAELEGVAVTAPRKGRSVEKENVAVES
EKNLGPSRRSPGTPRPPGASKGGRTPPQQGGRAGMGRASRSWEGSPGEQPRGGGAGGRGRRGRGRGSPHL
SGAGDTSISDRKSKEWEERRRQNIEKMNEEMEKIAEYERNQREGVLEPNPVRNFLDDPRRRSGPLEESER
DRREESRRHGRNWGGPDFERVRCGLEHERQGRRAGLGSAGDMTLSMTGRERSEYLRWKQEREKIDQERLQ
RHRKPTGQWRREWDAEKTDGMFKDGPVPAHEPSHRYDDQAWARPPKPPTFGEFLSQHKAEASSRRRRKSS
RPQAKAAPRAYSDHDDRWETKEGAASPAPETPQPTSPETSPKETPMQPPEIPAPAHRPPEDEGEENEGEE
DEEWEDISEDEEEEEIEVEEGDEEEPAQDHQAPEAAPTGIPCSEQAHGVPFSPEEPLLEPQAPGTPSSPF
SPPSGHQPVSDWGEEVELNSPRTTHLAGALSPGEAWPFESV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_015603
ORF Size 1593 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015603.3
RefSeq Size 2026 bp
RefSeq ORF 1596 bp
Locus ID 26093
UniProt ID Q9Y3X0
Cytogenetics 19q13.32
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Citations

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