RHBDD3 (NM_012265) Human Tagged ORF Clone

  • TrueORF®
SKU
RG201105
RHBDD3 (tGFP-tagged) - Human rhomboid domain containing 3 (RHBDD3)
$489.00 $657.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RHBDD3
Synonyms C22orf3; HS984G1A; PTAG
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG201105 representing NM_012265
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCATGCCAGGGGCCCCCATGGCCAACTGTCCCCAGCACTGCCTCTGGCCTCCTCAGTCCTGATGCTGC
TGATGAGCACCCTGTGGCTGGTGGGGGCCGGCCCCGGCCTGGTCCTGGCCCCGGAGCTGTTGCTGGACCC
CTGGCAGGTGCACCGGCTGCTGACCCATGCCCTGGGCCACACGGCCCTGCCAGGCCTGCTCCTGAGCCTG
CTGCTCCTGCCCACTGTGGGCTGGCAGCAGGAGTGCCACCTGGGCACGCTGAGATTCCTGCATGCCTCAG
CCCTGCTCGCCCTGGCTTCTGGGCTGCTGGCAGTGCTGCTGGCAGGCCTTGGGCTGTCCAGTGCAGCCGG
CAGCTGTGGATACATGCCTGTCCACCTGGCCATGCTGGCTGGGGAGGGACACCGCCCTAGACGGCCCCGT
GGGGCACTGCCACCGTGGCTGTCGCCGTGGCTGCTGCTTGCCCTGACCCCACTGCTCAGCTCTGAGCCAC
CCTTCCTGCAGCTCCTTTGCGGCCTCCTTGCCGGCCTGGCCTATGCAGCTGGGGCCTTCCGGTGGCTGGA
ACCCTCAGAGCGACGGCTGCAGGTGCTGCAGGAGGGCGTCTTGTGCAGGACCTTGGCGGGGTGCTGGCCC
CTGAGGCTCCTTGCCACCCCGGGTAGCCTGGCGGAGCTGCCTGTCACCCATCCTGCCGGAGTGAGGCCTC
CCATCCCTGGACCGCCTTATGTGGCCTCCCCTGACCTCTGGTCCCACTGGGAAGACTCAGCCCTGCCCCC
ACCAAGCCTGAGGCCTGTGCAGCCCACCTGGGAGGGCTCCTCAGAGGCAGGCCTGGACTGGGCTGGGGCC
AGCTTCTCCCCAGGGACTCCGATGTGGGCGGCCTTGGATGAGCAGATGCTGCAGGAGGGCATCCAGGCCT
CGCTTCTTGACGGGCCAGCCCAGGAACCCCAGAGCGCACCATGGCTGTCCAAGTCCTCTGTCTCCTCTCT
GCGGCTGCAGCAGCTGGAGCGCATGGGCTTCCCTACGGAGCAGGCGGTGGTGGCACTGGCAGCCACAGGC
CGTGTGGAGGGTGCCGTGTCACTGTTGGTTGGAGGACAAGTGGGCACTGAGACCCTGGTGACCCATGGAA
AGGGTGGGCCTGCCCACTCCGAGGGTCCTGGGCCTCCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG201105 representing NM_012265
Red=Cloning site Green=Tags(s)

MHARGPHGQLSPALPLASSVLMLLMSTLWLVGAGPGLVLAPELLLDPWQVHRLLTHALGHTALPGLLLSL
LLLPTVGWQQECHLGTLRFLHASALLALASGLLAVLLAGLGLSSAAGSCGYMPVHLAMLAGEGHRPRRPR
GALPPWLSPWLLLALTPLLSSEPPFLQLLCGLLAGLAYAAGAFRWLEPSERRLQVLQEGVLCRTLAGCWP
LRLLATPGSLAELPVTHPAGVRPPIPGPPYVASPDLWSHWEDSALPPPSLRPVQPTWEGSSEAGLDWAGA
SFSPGTPMWAALDEQMLQEGIQASLLDGPAQEPQSAPWLSKSSVSSLRLQQLERMGFPTEQAVVALAATG
RVEGAVSLLVGGQVGTETLVTHGKGGPAHSEGPGPP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_012265
ORF Size 1158 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012265.3
RefSeq Size 1738 bp
RefSeq ORF 1161 bp
Locus ID 25807
UniProt ID Q9Y3P4
Cytogenetics 22q12.2
Protein Families Druggable Genome, Transmembrane
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Documents
Resources
"NM_012265" in other vectors (4)
SKU Description Size Price
RC201105 RHBDD3 (Myc-DDK-tagged)-Human rhomboid domain containing 3 (RHBDD3) 10 ug
$457.00
RC201105L3 Lenti ORF clone of Human rhomboid domain containing 3 (RHBDD3), Myc-DDK-tagged 10 ug
$757.00
RC201105L4 Lenti ORF clone of Human rhomboid domain containing 3 (RHBDD3), mGFP tagged 10 ug
$757.00
SC319085 RHBDD3 (untagged)-Human rhomboid domain containing 3 (RHBDD3) 10 ug
$457.00

Plasmid Map for RG201105

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Cloning scheme for RG201105

Circular map for RG201105

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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