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TOR3A (NM_022371) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG200896
TOR3A (tGFP-tagged) - Human torsin family 3, member A (TOR3A)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
  $886.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TOR3A
Synonyms ADIR; ADIR2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG200896 representing NM_022371
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTTCGCGGTCCGTGGCGCCAGCTTTGGCTCTTTTTCCTGCTGCTGCTCCCGGGCGCGCCTGAGCCCC
GCGGCGCCTCCAGGCCGTGGGAGGGAACCGACGAGCCGGGCTCGGCCTGGGCCTGGCCGGGCTTCCAGCG
CCTGCAGGAGCAGCTCAGGGCGGCGGGTGCCCTCTCCAAGCGGTACTGGACGCTCTTCAGCTGCCAGGTG
TGGCCCGACGACTGTGACGAGGACGAGGAGGCAGCCACGGGGCCCCTGGGCTGGCGCCTTCCTCTGTTGG
GCCAGCGGTACCTGGACCTCCTGACCACGTGGTACTGCAGCTTCAAAGACTGCTGCCCTAGAGGGGATTG
CAGAATCTCCAACAACTTTACAGGCTTAGAGTGGGACCTGAATGTGCGGCTGCATGGCCAGCATTTGGTC
CAGCAGCTGGTCCTAAGAACAGTGAGGGGCTACTTAGAGACGCCCCAGCCAGAAAAGGCCCTTGCTCTGT
CGTTCCACGGCTGGTCTGGCACAGGCAAGAACTTCGTGGCACGGATGCTGGTGGAGAACCTGTATCGGGA
CGGGCTGATGAGTGACTGTGTCAGGATGTTCATCGCCACGTTCCACTTTCCTCACCCCAAATATGTGGAC
CTGTACAAGGAGCAGCTGATGAGCCAGATCCGGGAGACGCAGCAGCTCTGCCACCAGACCCTGTTCATCT
TCGATGAAGCGGAGAAGCTGCACCCAGGGCTGCTGGAGGTCCTTGGGCCACACTTAGAACGCCGGGCCCC
TGAGGGCCACAGGGCTGAGTCTCCATGGACTATCTTTCTGTTTCTCAGTAATCTCAGGGGCGATATAATC
AATGAGGTGGTCCTAAAGTTGCTCAAGGCTGGATGGTCCCGGGAAGAAATTACGATGGAACACCTGGAGC
CCCACCTCCAGGCGGAGATTGTGGAGACCATAGACAATGGCTTTGGCCACAGCCGTCTTGTGAAGGAAAA
CCTGATTGACTACTTCATCCCCTTCCTGCCTTTGGAGTACCGTCACGTGAGGCTGTGTGCACGGGATGCC
TTCCTGAGCCAGGAGCTCCTGTATAAAGAAGAGACACTGGATGAAATAGCCCAGATGATGGTGTATGTCC
CCAAGGAGGAACAACTCTTTTCTTCCCAGGGCTGCAAGTCTATTTCCCAGAGGATTAACTACTTCCTGTC
A

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG200896 representing NM_022371
Red=Cloning site Green=Tags(s)

MLRGPWRQLWLFFLLLLPGAPEPRGASRPWEGTDEPGSAWAWPGFQRLQEQLRAAGALSKRYWTLFSCQV
WPDDCDEDEEAATGPLGWRLPLLGQRYLDLLTTWYCSFKDCCPRGDCRISNNFTGLEWDLNVRLHGQHLV
QQLVLRTVRGYLETPQPEKALALSFHGWSGTGKNFVARMLVENLYRDGLMSDCVRMFIATFHFPHPKYVD
LYKEQLMSQIRETQQLCHQTLFIFDEAEKLHPGLLEVLGPHLERRAPEGHRAESPWTIFLFLSNLRGDII
NEVVLKLLKAGWSREEITMEHLEPHLQAEIVETIDNGFGHSRLVKENLIDYFIPFLPLEYRHVRLCARDA
FLSQELLYKEETLDEIAQMMVYVPKEEQLFSSQGCKSISQRINYFLS

TRTRPLE - GFP Tag - V
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_022371
ORF Size 1191 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_022371.4
RefSeq Size 2138 bp
RefSeq ORF 1194 bp
Locus ID 64222
UniProt ID Q9H497
Cytogenetics 1q25.2
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Documents
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"NM_022371" in other vectors (4)
SKU Description Size Price
RC200896 TOR3A (Myc-DDK-tagged)-Human torsin family 3, member A (TOR3A) 10 ug
$686.00
RC200896L3 Lenti-ORF clone of TOR3A (Myc-DDK-tagged)-Human torsin family 3, member A (TOR3A) 10 ug
$986.00
RC200896L4 Lenti-ORF clone of TOR3A (mGFP-tagged)-Human torsin family 3, member A (TOR3A) 10 ug
$986.00
SC320221 TOR3A (untagged)-Human torsin family 3, member A (TOR3A) 10 ug
$686.00

Plasmid Map for RG200896

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG200896
Circular map for RG200896

file-alt Citations

HIV-host interactome revealed directly from infected cells
Luo, Jacobs, Greco et al
Nat Microbiol (2016) 1 (7) DOI: 10.1038/nmicrobiol.2016.68
Cited by 57
AI Summary: OriGene products—including TAP2 (RC239305), AMBRA1 (RC206255), and TOR3A (SC320221 and RG200896) expression plasmids—were used to express and study host proteins in the investigation of HIV-1 protein interactions with cellular factors during infection.
Snippet: OriGene: TAP2 (pCMV6-Entry-TAP2-Myc-DDK;RC239305), AMBRA1 (pCMV6-Entry-AMBRA1-Myc-DDK;RC206255), TOR3A (pCMV6-AC-TOR3A. untagged;SC320221, pCMV6-AC-tGFP-TOR3A;RG200896). Clontech: pEGFP-N1 (6085-1). The TOR3A coding region was amplified by PCR from pCMV6-AC-tGFP-TOR3A (Origene #RG200896) and cloned into pEGFP-N1
HIV–host interactome revealed directly from infected cells
Luo, Jacobs, Greco et al
Nature microbiology (2016)
AI Summary: OriGene products contributed to this study by providing plasmid constructs for TAP2 (pCMV6-Entry-TAP2-Myc-DDK; RC239305), AMBRA1 (pCMV6-Entry-AMBRA1-Myc-DDK; RC206255), and TOR3A (pCMV6-AC-TOR3A; SC320221 and pCMV6-AC-tGFP-TOR3A; RG200896), which were used to investigate host protein interactions with HIV in infected cells.
Snippet: … OriGene: TAP2 (pCMV6-Entry-TAP2-Myc-DDK;RC239305), AMBRA1 (pCMV6-Entry-AMBRA1-Myc-DDK;RC206255), TOR3A (pCMV6-AC-TOR3A. untagged;SC320221, pCMV6-AC-tGFP-TOR3A;RG200896). …
Showing 1-2 of 2 papers.
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