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ELAC2 (NM_018127) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG200764
ELAC2 (tGFP-tagged) - Human elaC homolog 2 (E. coli) (ELAC2), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$789.00 $956.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ELAC2
Synonyms COXPD17; ELC2; HPC2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG200764 representing NM_018127
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGGCGCTTTGCTCGCTGCTGCGGTCCGCGGCCGGACGCACCATGTCGCAGGGACGCACCATATCGC
AGGCACCCGCCCGCCGCGAGCGGCCGCGCAAGGACCCGCTGCGGCACCTGCGCACGCGAGAGAAGCGCGG
ACCGTCGGGGTGCTCCGGCGGCCCAAACACCGTGTACCTGCAGGTGGTGGCAGCGGGTAGCCGGGACTCG
GGCGCCGCGCTCTACGTCTTCTCCGAGTTCAACCGGTATCTCTTCAACTGTGGAGAAGGCGTTCAGAGAC
TCATGCAGGAGCACAAGTTAAAGGTTGCTCGCCTGGACAACATATTCCTGACACGAATGCACTGGTCTAA
TGTTGGGGGCTTAAGTGGAATGATTCTTACTTTAAAGGAAACCGGGCTTCCAAAGTGTGTACTTTCTGGA
CCTCCACAACTGGAAAAATACCTCGAAGCAATCAAAATATTTTCTGGTCCATTGAAAGGAATAGAACTGG
CTGTGCGGCCCCACTCTGCCCCAGAATACGAGGATGAAACCATGACAGTTTACCAGATCCCCATACACAG
TGAACAGAGGAGGGGAAAGCACCAACCATGGCAGAGTCCAGAAAGGCCTCTCAGCAGGCTCAGTCCAGAG
CGATCTTCAGACTCCGAGTTGAATGAAAATGAGCCACACCTTCCACATGGTGTTAGCCAGAGAAGAGGGG
TCAGGGACTCTTCCCTGGTCGTAGCTTTCATCTGTAAGCTTCACTTAAAGAGAGGAAACTTCTTGGTGCT
CAAAGCAAAGGAGATGGGCCTCCCAGTTGGGACAGCTGCCATCGCTCCCATCATTGCTGCTGTCAAGGAC
GGGAAAAGCATCACTCATGAAGGAAGAGAGATTTTGGCTGAAGAGCTGTGTACTCCTCCAGATCCTGGTG
CTGCTTTTGTGGTGGTAGAATGTCCAGATGAAAGCTTCATTCAACCCATCTGTGAGAATGCCACCTTTCA
GAGGTACCAAGGAAAGGCAGATGCCCCCGTGGCCTTGGTGGTTCACATGGCCCCAGCATCTGTGCTTGTG
GACAGCAGGTACCAGCAGTGGATGGAGAGGTTTGGGCCTGACACCCAGCACTTGGTCCTGAATGAGAACT
GTGCCTCAGTTCACAACCTTCGCAGCCACAAGATTCAAACCCAGCTCAACCTCATCCACCCGGACATCTT
CCCCCTGCTCACCAGTTTCCGCTGTAAGAAGGAGGGCCCCACCCTCAGTGTGCCCATGGTTCAGGGTGAA
TGCCTCCTCAAGTACCAGCTCCGTCCCAGGAGGGAGTGGCAGAGGGATGCCATTATTACTTGCAATCCTG
AGGAATTCATAGTTGAGGCGCTGCAGCTTCCCAACTTCCAGCAGAGCGTGCAGGAGTACAGGAGGAGTGC
GCAGGACGGCCCAGCCCCAGCAGAGAAAAGAAGTCAGTACCCAGAAATCATCTTCCTTGGAACAGGGTCT
GCCATCCCGATGAAGATTCGAAATGTCAGTGCCACACTTGTCAACATAAGCCCCGACACGTCTCTGCTAC
TGGACTGTGGTGAGGGCACGTTTGGGCAGCTGTGCCGTCATTACGGAGACCAGGTGGACAGGGTCCTGGG
CACCCTGGCTGCTGTGTTTGTGTCCCACCTGCACGCAGATCACCACACGGGCTTGCCAAGTATCTTGCTG
CAGAGAGAACGCGCCTTGGCATCTTTGGGAAAGCCGCTTCACCCTTTGCTGGTGGTTGCCCCCAACCAGC
TCAAAGCCTGGCTCCAGCAGTACCACAACCAGTGCCAGGAGGTCCTGCACCACATCAGTATGATTCCTGC
CAAATGCCTTCAGGAAGGGGCTGAGATCTCCAGTCCTGCAGTGGAAAGATTGATCAGTTCGCTGTTGCGA
ACATGTGATTTGGAAGAGTTTCAGACCTGTCTGGTGCGGCACTGCAAGCATGCGTTTGGCTGTGCGCTGG
TGCACACCTCTGGCTGGAAAGTGGTCTATTCCGGGGACACCATGCCCTGCGAGGCTCTGGTCCGGATGGG
GAAAGATGCCACCCTCCTGATACATGAAGCCACCCTGGAAGATGGTTTGGAAGAGGAAGCAGTGGAAAAG
ACACACAGCACAACGTCCCAAGCCATCAGCGTGGGGATGCGGATGAACGCGGAGTTCATTATGCTGAACC
ACTTCAGCCAGCGCTATGCCAAGGTCCCCCTCTTCAGCCCCAACTTCAGCGAGAAAGTGGGAGTTGCCTT
TGACCACATGAAGGTCTGCTTTGGAGACTTTCCAACAATGCCCAAGCTGATTCCCCCACTGAAAGCCCTG
TTTGCTGGCGACATCGAGGAGATGGAGGAGCGCAGGGAGAAGCGGGAGCTGCGGCAGGTGCGGGCGGCCC
TCCTGTCCAGGGAGCTGGCAGGCGGCCTGGAGGATGGGGAGCCTCAGCAGAAGCGGGCCCACACAGAGGA
GCCACAGGCCAAGAAGGTCAGAGCCCAG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG200764 representing NM_018127
Red=Cloning site Green=Tags(s)

MWALCSLLRSAAGRTMSQGRTISQAPARRERPRKDPLRHLRTREKRGPSGCSGGPNTVYLQVVAAGSRDS
GAALYVFSEFNRYLFNCGEGVQRLMQEHKLKVARLDNIFLTRMHWSNVGGLSGMILTLKETGLPKCVLSG
PPQLEKYLEAIKIFSGPLKGIELAVRPHSAPEYEDETMTVYQIPIHSEQRRGKHQPWQSPERPLSRLSPE
RSSDSELNENEPHLPHGVSQRRGVRDSSLVVAFICKLHLKRGNFLVLKAKEMGLPVGTAAIAPIIAAVKD
GKSITHEGREILAEELCTPPDPGAAFVVVECPDESFIQPICENATFQRYQGKADAPVALVVHMAPASVLV
DSRYQQWMERFGPDTQHLVLNENCASVHNLRSHKIQTQLNLIHPDIFPLLTSFRCKKEGPTLSVPMVQGE
CLLKYQLRPRREWQRDAIITCNPEEFIVEALQLPNFQQSVQEYRRSAQDGPAPAEKRSQYPEIIFLGTGS
AIPMKIRNVSATLVNISPDTSLLLDCGEGTFGQLCRHYGDQVDRVLGTLAAVFVSHLHADHHTGLPSILL
QRERALASLGKPLHPLLVVAPNQLKAWLQQYHNQCQEVLHHISMIPAKCLQEGAEISSPAVERLISSLLR
TCDLEEFQTCLVRHCKHAFGCALVHTSGWKVVYSGDTMPCEALVRMGKDATLLIHEATLEDGLEEEAVEK
THSTTSQAISVGMRMNAEFIMLNHFSQRYAKVPLFSPNFSEKVGVAFDHMKVCFGDFPTMPKLIPPLKAL
FAGDIEEMEERREKRELRQVRAALLSRELAGGLEDGEPQQKRAHTEEPQAKKVRAQ

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018127
ORF Size 2478 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018127.4, NP_060597.3
RefSeq Size 2997 bp
RefSeq ORF 2481 bp
Locus ID 60528
UniProt ID Q9BQ52
Cytogenetics 17p12
Domains lactamase_B
Protein Families Druggable Genome, Stem cell - Pluripotency
Summary The protein encoded by this gene has a C-terminal domain with tRNA 3′ processing endoribonuclease activity, which catalyzes the removal of the 3' trailer from precursor tRNAs. The protein also interacts with activated Smad family member 2 (Smad2) and its nuclear partner forkhead box H1 (also known as FAST-1), and reduced expression can suppress transforming growth factor-beta induced growth arrest. Mutations in this gene result in an increased risk of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Sep 2009
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Documents
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"NM_018127" in other vectors (6)
SKU Description Size Price
RC200764 ELAC2 (Myc-DDK-tagged)-Human elaC homolog 2 (E. coli) (ELAC2), transcript variant 1 10 ug
$756.00
RC200764L1 Lenti ORF clone of Human elaC homolog 2 (E. coli) (ELAC2), transcript variant 1, Myc-DDK-tagged 10 ug
$1,056.00
RC200764L2 Lenti ORF clone of Human elaC homolog 2 (E. coli) (ELAC2), transcript variant 1, mGFP tagged 10 ug
$1,056.00
RC200764L3 Lenti ORF clone of Human elaC homolog 2 (E. coli) (ELAC2), transcript variant 1, Myc-DDK-tagged 10 ug
$1,056.00
RC200764L4 Lenti ORF clone of Human elaC homolog 2 (E. coli) (ELAC2), transcript variant 1, mGFP tagged 10 ug
$1,056.00
SC113690 ELAC2 (untagged)-Human elaC homolog 2 (E. coli) (ELAC2), transcript variant 1 10 ug
$757.00

Plasmid Map for RG200764

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG200764
Circular map for RG200764

file-alt Citations

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