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SURF6 (NM_006753) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG200741
SURF6 (tGFP-tagged) - Human surfeit 6 (SURF6)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $657.00
3 Weeks*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SURF6
Synonyms RRP14
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG200741 representing NM_006753
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTCTCTACTCGCCAAGGACGCCTACCTGCAGAGCCTGGCCAAGAAGATCTGCTCCCATTCGGCCC
CGGAACAGCAGGCGCGCACGCGGGCTGGCAAAACTCAAGGCTCAGAAACTGCAGGGCCCCCAAAAAAGAA
AAGGAAGAAAACACAAAAGAAATTCCGGAAGCGAGAAGAGAAGGCTGCTGAGCACAAGGCCAAGTCCTTG
GGGGAGAAATCTCCAGCAGCCTCTGGGGCCAGGAGGCCTGAGGCAGCCAAAGAGGAAGCAGCTTGGGCTT
CCAGCTCAGCAGGGAACCCTGCAGATGGCCTGGCCACTGAGCCTGAGTCTGTCTTTGCTCTGGATGTTCT
GCGACAGCGACTGCATGAGAAGATCCAGGAGGCCCGGGGCCAGGGCAGTGCCAAGGAGCTGTCCCCTGCC
GCCTTGGAGAAAAGGCGGCGGAGAAAGCAGGAACGGGACCGGAAGAAGAGGAAGCGAAAGGAGCTGCGGG
CGAAAGAGAAGGCCAGGAAGGCTGAGGAGGCCACGGAGGCCCAGGAGGTGGTGGAGGCAACCCCAGAGGG
GGCCTGCACGGAGCCGCGGGAGCCGCCCGGGCTGATCTTCAATAAGGTGGAGGTGAGCGAAGACGAGCCG
GCCAGCAAGGCGCAGCGCAGAAAAGAGAAGAGGCAGAGGGTGAAGGGGAACCTCACGCCGCTGACCGGGA
GGAACTACCGGCAGCTGCTGGAGCGCCTGCAGGCACGGCAGAGCCGGCTGGACGAGCTGCGCGGCCAGGA
TGAGGGGAAGGCGCAGGAGCTGGAGGCGAAGATGAAGTGGACCAACCTCCTCTACAAGGCGGAGGGCGTG
AAGATCCGTGACGACGAACGCCTGCTGCAGGAGGCCCTGAAGCGCAAGGAGAAGCGCAGGGCGCAGCGGC
AGCGCCGGTGGGAGAAGCGCACGGCCGGCGTGGTGGAGAAGATGCAGCAGCGCCAGGACCGGCGGCGGCA
GAACCTGCGCAGGAAGAAGGCGGCCCGCGCCGAGCGCCGCCTGCTCAGAGCCCGCAAGAAGGGCCGCATC
CTGCCGCAGGACCTGGAGCGCGCAGGCCTGGTC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG200741 representing NM_006753
Red=Cloning site Green=Tags(s)

MASLLAKDAYLQSLAKKICSHSAPEQQARTRAGKTQGSETAGPPKKKRKKTQKKFRKREEKAAEHKAKSL
GEKSPAASGARRPEAAKEEAAWASSSAGNPADGLATEPESVFALDVLRQRLHEKIQEARGQGSAKELSPA
ALEKRRRRKQERDRKKRKRKELRAKEKARKAEEATEAQEVVEATPEGACTEPREPPGLIFNKVEVSEDEP
ASKAQRRKEKRQRVKGNLTPLTGRNYRQLLERLQARQSRLDELRGQDEGKAQELEAKMKWTNLLYKAEGV
KIRDDERLLQEALKRKEKRRAQRQRRWEKRTAGVVEKMQQRQDRRRQNLRRKKAARAERRLLRARKKGRI
LPQDLERAGLV

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006753
ORF Size 1083 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006753.6
RefSeq Size 2349 bp
RefSeq ORF 1086 bp
Locus ID 6838
UniProt ID O75683
Cytogenetics 9q34.2
Domains SURF6
Summary This gene encodes a conserved protein that is localized to the nucleolus. The encoded protein may function as a nucleolar-matrix protein with nucleic acid-binding properties. There is a pseudogene for this gene on chromosome Y. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2013
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"NM_006753" in other vectors (4)
SKU Description Size Price
RC200741 SURF6 (Myc-DDK-tagged)-Human surfeit 6 (SURF6) 10 ug
$457.00
RC200741L3 Lenti ORF clone of Human surfeit 6 (SURF6), Myc-DDK-tagged 10 ug
$757.00
RC200741L4 Lenti ORF clone of Human surfeit 6 (SURF6), mGFP tagged 10 ug
$757.00
SC115907 SURF6 (untagged)-Human surfeit 6 (SURF6) 10 ug
$457.00

Plasmid Map for RG200741

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG200741
Circular map for RG200741

file-alt Citations

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