ABI2 (NM_005759) Human Tagged ORF Clone

SKU RG200637

ABI2 (tGFP-tagged) - Human abl-interactor 2 (ABI2)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: ABI2
• Synonyms: ABI-2; ABI2B; AblBP3; AIP-1; AIP1; argBP1; argBPIA; argBPIB; SSH3BP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200637 representing NM_005759; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGAGCTGCAGATGCTGCTGGAAGAGGAAATCCCGGGGGGCCGCCGGGCCCTCTTCGACAGCTACA; CAAATCTGGAACGGGTGGCCGATTACTGCGAGAACAACTACATACAGTCAGCAGATAAGCAGAGAGCCCT; AGAAGAAACCAAAGCCTACACCACCCAATCCTTAGCAAGTGTTGCCTATCTGATAAACACCTTGGCCAAC; AATGTCCTGCAGATGCTGGATATCCAGGCATCCCAGCTACGAAGGATGGAATCTTCAATCAATCATATTT; CACAAACAGTTGATATTCATAAAGAGAAAGTTGCAAGAAGAGAAATTGGTATTTTGACTACCAATAAAAA; CACTTCAAGGACACATAAGATTATTGCTCCAGCCAACCTTGAACGACCAGTTCGTTATATTAGAAAACCT; ATTGACTATACAATTCTAGATGATATTGGACATGGAGTAAAGGTGAGTACCCAGAACATGAAGATGGGTG; GGCTGCCGCGTACAACACCTCCAACTCAGAAGCCCCCTAGTCCCCCTATGTCAGGGAAAGGGACACTTGG; GCGGCACTCCCCCTATCGCACACTGGAGCCAGTGCGTCCTCCAGTGGTACCAAATGATTACGTACCTAGC; CCAACCCGTAATATGGCTCCCTCGCAGCAGAGCCCTGTGAGGACAGCTTCTGTGAATCAAAGAAATCGAA; CTTACAGCAGCAGTGGGAGTAGTGGAGGGAGCCACCCAAGTAGTCGGAGCAGCAGTCGAGAGAACAGTGG; AAGTGGTAGTGTGGGGGTTCCTATTGCTGTTCCTACTCCATCTCCTCCCAGTGTCTTTCCAGGTCATCCT; GTACAGTTCTACAGCATGAATAGGCCTGCCTCTCGCCATACTCCCCCAACAATAGGGGGCTCGTTGCCCT; ATAGACGCCCTCCTTCCATTACTTCACAAACAAGCCTTCAGAATCAGATGAATGGAGGACCTTTTTATAG; CCAGAATCCAGTATCTCTTGCTCCTCCTCCTCCCTCCATCCTACAGGTAACTCCTCAGTTACCTTTAATG; GGATTTGTGGCCAGAGTCCAAGAAAATATTTCAGATACACCACCTCCACCGCCACCTGTGGAAGAACCAG; TCTTTGATGAGTCTCCCCCACCTCCTCCTCCTCCAGAAGATTACGAAGAGGAGGAAGCTGCTGTGGTTGA; GTATAGTGATCCTTATGCTGAAGAGGACCCACCGTGGGCTCCACGTTCTTACTTGGAAAAGGTTGTGGCA; ATTTATGACTATACAAAAGACAAGGAAGATGAGCTGTCCTTTCAGGAAGGAGCCATTATTTATGTCATCA; AGAAGAATGACGATGGTTGGTATGAGGGAGTTATGAATGGAGTGACTGGGCTTTTTCCTGGGAATTACGT; TGAGTCTATCATGCATTATTCTGAG; ; ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200637 representing NM_005759; Red=Cloning site Green=Tags(s); ; MAELQMLLEEEIPGGRRALFDSYTNLERVADYCENNYIQSADKQRALEETKAYTTQSLASVAYLINTLAN; NVLQMLDIQASQLRRMESSINHISQTVDIHKEKVARREIGILTTNKNTSRTHKIIAPANLERPVRYIRKP; IDYTILDDIGHGVKVSTQNMKMGGLPRTTPPTQKPPSPPMSGKGTLGRHSPYRTLEPVRPPVVPNDYVPS; PTRNMAPSQQSPVRTASVNQRNRTYSSSGSSGGSHPSSRSSSRENSGSGSVGVPIAVPTPSPPSVFPGHP; VQFYSMNRPASRHTPPTIGGSLPYRRPPSITSQTSLQNQMNGGPFYSQNPVSLAPPPPSILQVTPQLPLM; GFVARVQENISDTPPPPPPVEEPVFDESPPPPPPPEDYEEEEAAVVEYSDPYAEEDPPWAPRSYLEKVVA; IYDYTKDKEDELSFQEGAIIYVIKKNDDGWYEGVMNGVTGLFPGNYVESIMHYSE; ; TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005759
• ORF Size: 1425 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005759.7
• RefSeq Size: 6489 bp
• RefSeq ORF: 1428 bp
• Locus ID: 10152
• UniProt ID: Q9NYB9
• Cytogenetics: 2q33.2
• Domains: SH3
• Protein Pathways: Regulation of actin cytoskeleton
• Summary: Regulator of actin cytoskeleton dynamics underlying cell motility and adhesion. Functions as a component of the WAVE complex, which activates actin nucleating machinery Arp2/3 to drive lamellipodia formation (PubMed:21107423). Acts as regulator and substrate of nonreceptor tyrosine kinases ABL1 and ABL2 involved in processes linked to cell growth and differentiation. Positively regulates ABL1-mediated phosphorylation of ENAH, which is required for proper polymerization of nucleated actin filaments at the leading edge (PubMed:7590236, PubMed:8649853, PubMed:10498863). Contributes to the regulation of actin assembly at the tips of neuron projections. In particular, controls dendritic spine morphogenesis and may promote dendritic spine specification toward large mushroom-type spines known as repositories of memory in the brain (By similarity). In hippocampal neurons, may mediate actin-dependent BDNF-NTRK2 early endocytic trafficking that triggers dendrite outgrowth (By similarity). Participates in ocular lens morphogenesis, likely by regulating lamellipodia-driven adherens junction formation at the epithelial cell-secondary lens fiber interface (By similarity). Also required for nascent adherens junction assembly in epithelial cells (PubMed:15572692).[UniProtKB/Swiss-Prot Function]