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DUSP14 (NM_007026) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG200531
DUSP14 (tGFP-tagged) - Human dual specificity phosphatase 14 (DUSP14)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $500.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "DUSP14 or NM_007026"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DUSP14
Synonyms MKP-L; MKP6
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG200531 representing NM_007026
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTCCAGAGGTCACAGCACGCTACCAAGGACTCTCATGGCCCCTCGGATGATTTCCGAGGGAGACA
TAGGAGGCATTGCTCAAATCACCTCCTCTCTATTCCTGGGCAGAGGCAGTGTGGCCTCCAATCGGCACCT
CCTCCAGGCTCGTGGCATCACCTGCATTGTTAATGCTACCATTGAGATCCCTAATTTCAACTGGCCCCAA
TTTGAGTATGTTAAAGTGCCTCTGGCTGACATGCCGCATGCCCCCATTGGACTGTACTTTGACACCGTGG
CTGACAAGATCCACAGTGTGAGCAGGAAGCACGGGGCCACCTTGGTGCACTGTGCTGCAGGGGTGAGCCG
CTCAGCCACGCTGTGTATCGCGTACCTGATGAAATTCCACAACGTGTGCCTGCTGGAGGCGTACAACTGG
GTGAAAGCCCGGCGACCTGTCATCAGGCCCAACGTAGGCTTCTGGAGGCAACTGATAGACTACGAGCGCC
AGCTCTTTGGGAAGTCGACAGTTAAAATGGTACAGACACCTTATGGCATAGTTCCCGACGTCTATGAGAA
GGAGTCCCGACACCTGATGCCTTACTGGGGGATT

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG200531 representing NM_007026
Red=Cloning site Green=Tags(s)

MSSRGHSTLPRTLMAPRMISEGDIGGIAQITSSLFLGRGSVASNRHLLQARGITCIVNATIEIPNFNWPQ
FEYVKVPLADMPHAPIGLYFDTVADKIHSVSRKHGATLVHCAAGVSRSATLCIAYLMKFHNVCLLEAYNW
VKARRPVIRPNVGFWRQLIDYERQLFGKSTVKMVQTPYGIVPDVYEKESRHLMPYWGI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_007026
ORF Size 594 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_007026.4
RefSeq Size 1471 bp
RefSeq ORF 597 bp
Locus ID 11072
UniProt ID O95147
Cytogenetics 17q12
Domains DSPc
Protein Families Druggable Genome, Phosphatase
Protein Pathways MAPK signaling pathway
Summary Dual-specificity phosphatases (DUSPs) constitute a large heterogeneous subgroup of the type I cysteine-based protein-tyrosine phosphatase superfamily. DUSPs are characterized by their ability to dephosphorylate both tyrosine and serine/threonine residues. They have been implicated as major modulators of critical signaling pathways. DUSP14 contains the consensus DUSP C-terminal catalytic domain but lacks the N-terminal CH2 domain found in the MKP (mitogen-activated protein kinase phosphatase) class of DUSPs (see MIM 600714) (summary by Patterson et al., 2009 PubMed 19228121).supplied by OMIM, Dec 2009
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"NM_007026" in other vectors (6)
SKU Description Size Price
RC200531 DUSP14 (Myc-DDK-tagged)-Human dual specificity phosphatase 14 (DUSP14) 10 ug
$300.00
RC200531L1 Lenti ORF clone of Human dual specificity phosphatase 14 (DUSP14), Myc-DDK-tagged 10 ug
$600.00
RC200531L2 Lenti ORF clone of Human dual specificity phosphatase 14 (DUSP14), mGFP tagged 10 ug
$600.00
RC200531L3 Lenti ORF clone of Human dual specificity phosphatase 14 (DUSP14), Myc-DDK-tagged 10 ug
$600.00
RC200531L4 Lenti ORF clone of Human dual specificity phosphatase 14 (DUSP14), mGFP tagged 10 ug
$600.00
SC115747 DUSP14 (untagged)-Human dual specificity phosphatase 14 (DUSP14) 10 ug
$300.00

Plasmid Map for RG200531

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for RG200531
Circular map for RG200531

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