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HSF1 (NM_005526) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG200314
HSF1 (tGFP-tagged) - Human heat shock transcription factor 1 (HSF1)
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $938.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HSF1
Synonyms HSTF1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG200314 representing NM_005526
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCTGCCCGTGGGCCCCGGCGCGGCGGGGCCCAGCAACGTCCCGGCCTTCCTGACCAAGCTGTGGA
CCCTCGTGAGCGACCCGGACACCGACGCGCTCATCTGCTGGAGCCCGAGCGGGAACAGCTTCCACGTGTT
CGACCAGGGCCAGTTTGCCAAGGAGGTGCTGCCCAAGTACTTCAAGCACAACAACATGGCCAGCTTCGTG
CGGCAGCTCAACATGTATGGCTTCCGGAAAGTGGTCCACATCGAGCAGGGCGGCCTGGTCAAGCCAGAGA
GAGACGACACGGAGTTCCAGCACCCATGCTTCCTGCGTGGCCAGGAGCAGCTCCTTGAGAACATCAAGAG
GAAAGTGACCAGTGTGTCCACCCTGAAGAGTGAAGACATAAAGATCCGCCAGGACAGCGTCACCAAGCTG
CTGACGGACGTGCAGCTGATGAAGGGGAAGCAGGAGTGCATGGACTCCAAGCTCCTGGCCATGAAGCATG
AGAATGAGGCTCTGTGGCGGGAGGTGGCCAGCCTTCGGCAGAAGCATGCCCAGCAACAGAAAGTCGTCAA
CAAGCTCATTCAGTTCCTGATCTCACTGGTGCAGTCAAACCGGATCCTGGGGGTGAAGAGAAAGATCCCC
CTGATGCTGAACGACAGTGGCTCAGCACATTCCATGCCCAAGTATAGCCGGCAGTTCTCCCTGGAGCACG
TCCACGGCTCGGGCCCCTACTCGGCCCCCTCCCCAGCCTACAGCAGCTCCAGCCTCTACGCCCCTGATGC
TGTGGCCAGCTCTGGACCCATCATCTCCGACATCACCGAGCTGGCTCCTGCCAGCCCCATGGCCTCCCCC
GGCGGGAGCATAGACGAGAGGCCCCTATCCAGCAGCCCCCTGGTGCGTGTCAAGGAGGAGCCCCCCAGCC
CGCCTCAGAGCCCCCGGGTAGAGGAGGCGAGTCCCGGGCGCCCATCTTCCGTGGACACCCTCTTGTCCCC
GACCGCCCTCATTGACTCCATCCTGCGGGAGAGTGAACCTGCCCCCGCCTCCGTCACAGCCCTCACGGAC
GCCAGGGGCCACACGGACACCGAGGGCCGGCCTCCCTCCCCCCCGCCCACCTCCACCCCTGAAAAGTGCC
TCAGCGTAGCCTGCCTGGACAAGAATGAGCTCAGTGACCACTTGGATGCTATGGACTCCAACCTGGATAA
CCTGCAGACCATGCTGAGCAGCCACGGCTTCAGCGTGGACACCAGTGCCCTGCTGGACCTGTTCAGCCCC
TCGGTGACCGTGCCCGACATGAGCCTGCCTGACCTTGACAGCAGCCTGGCCAGTATCCAAGAGCTCCTGT
CTCCCCAGGAGCCCCCCAGGCCTCCCGAGGCAGAGAACAGCAGCCCGGATTCAGGGAAGCAGCTGGTGCA
CTACACAGCGCAGCCGCTGTTCCTGCTGGACCCCGGCTCCGTGGACACCGGGAGCAACGACCTGCCGGTG
CTGTTTGAGCTGGGAGAGGGCTCCTACTTCTCCGAAGGGGACGGCTTCGCCGAGGACCCCACCATCTCCC
TGCTGACAGGCTCGGAGCCTCCCAAAGCCAAGGACCCCACTGTCTCC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG200314 representing NM_005526
Red=Cloning site Green=Tags(s)

MDLPVGPGAAGPSNVPAFLTKLWTLVSDPDTDALICWSPSGNSFHVFDQGQFAKEVLPKYFKHNNMASFV
RQLNMYGFRKVVHIEQGGLVKPERDDTEFQHPCFLRGQEQLLENIKRKVTSVSTLKSEDIKIRQDSVTKL
LTDVQLMKGKQECMDSKLLAMKHENEALWREVASLRQKHAQQQKVVNKLIQFLISLVQSNRILGVKRKIP
LMLNDSGSAHSMPKYSRQFSLEHVHGSGPYSAPSPAYSSSSLYAPDAVASSGPIISDITELAPASPMASP
GGSIDERPLSSSPLVRVKEEPPSPPQSPRVEEASPGRPSSVDTLLSPTALIDSILRESEPAPASVTALTD
ARGHTDTEGRPPSPPPTSTPEKCLSVACLDKNELSDHLDAMDSNLDNLQTMLSSHGFSVDTSALLDLFSP
SVTVPDMSLPDLDSSLASIQELLSPQEPPRPPEAENSSPDSGKQLVHYTAQPLFLLDPGSVDTGSNDLPV
LFELGEGSYFSEGDGFAEDPTISLLTGSEPPKAKDPTVS

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_005526
ORF Size 1587 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005526.4
RefSeq Size 2166 bp
RefSeq ORF 1590 bp
Locus ID 3297
UniProt ID Q00613
Cytogenetics 8q24.3
Domains HSF
Protein Families Transcription Factors
Summary The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phosphorylation, which promotes binding by heat shock protein 90. provided by RefSeq, Jul 2017
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"NM_005526" in other vectors (6)
SKU Description Size Price
RC200314 HSF1 (Myc-DDK-tagged)-Human heat shock transcription factor 1 (HSF1) 10 ug
$738.00
RC200314L1 Lenti ORF clone of Human heat shock transcription factor 1 (HSF1), Myc-DDK-tagged 10 ug
$1,038.00
RC200314L2 Lenti ORF clone of Human heat shock transcription factor 1 (HSF1), mGFP tagged 10 ug
$1,038.00
RC200314L3 Lenti ORF clone of Human heat shock transcription factor 1 (HSF1), Myc-DDK-tagged 10 ug
$1,038.00
RC200314L4 Lenti ORF clone of Human heat shock transcription factor 1 (HSF1), mGFP tagged 10 ug
$1,038.00
SC321225 HSF1 (untagged)-Human heat shock transcription factor 1 (HSF1) 10 ug
$740.00

Plasmid Map for RG200314

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG200314
Circular map for RG200314

file-alt Citations

FAM3C-YY1 axis is essential for TGFβ-promoted proliferation and migration of human breast cancer MDA-MB-231 cells via the activation of HSF1
Yang, Feng, Meng et al
J. Cell. Mol. Med. (2019) 23 (5), 3464-3475 DOI: 10.1111/jcmm.14243
AI Summary: OriGene’s HSF1 plasmid (Cat No RG200314, in pCMV6-AC-GFP vector) was used to overexpress human HSF1 in breast cancer cells, enabling the study of its role in TGFβ-promoted proliferation and migration in the research.
Snippet: The mRNA and protein levels were analysed as above. Heat shock factor 1 plasmid expressing human HSF1 gene was purchased from OriGene27 (HSF1, Cat No RG200314, in pCMV6‐AC‐GFP vector, Rockville, MD, USA) and YY1 plasmid (in pCDNA3.1 vector) expressing mouse YY1 gene was kindly provided by Prof. Yan Lu of Fudan University, China
FigureFigureFigure
BAG3-dependent expression of Mcl-1 confers resistance of mutant KRAS colon cancer cells to the HSP90 inhibitor AUY922
Wang, Guo, Croft et al
Mol. Carcinog. (2017)
AI Summary: OriGene products pCMV6-AC-GFP-Mcl-1, pCMV6-AC-GFP-BAG3, and pCMV6-AC-GFP-HSF1 plasmid vectors were used in this study to overexpress the respective proteins and investigate their roles in conferring resistance to AUY922 in mutant KRAS colon cancer cells.
Snippet: Bethesda, MD). Plasmid vector and transfection The pCMV6-AC-GFP-Mcl-1, pCMV6-AC-GFP-BAG3 and pCMV6-AC-GFP-HSF1 vectors were from Origene (Australian Biosearch, WA, Australia). Transfection of cDNA was carried out as described previously [8]
Showing 1-2 of 2 papers.
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