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PLEKHB2 (NM_001031706) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG200155
PLEKHB2 (tGFP-tagged) - Human pleckstrin homology domain containing, family B (evectins) member 2 (PLEKHB2), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $500.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PLEKHB2
Synonyms EVT2; EVT2, FLJ20783; FLJ20783; FLJ23679; FLJ30436; pleckstrin homology domain containing, family B (evectins) member 2
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG200155 representing NM_001031706
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGTTTGTGAAGAGTGGCTGGTTGCTGCGACAGAGTACTATTTTGAAGCGCTGGAAGAAGAACTGGT
TTGATCTGTGGTCGGATGGTCACCTGATCTATTATGATGACCAGACTCGGCAGAATATCGAGGATAAGGT
CCACATGCCAATGGACTGCATCAACATCCGCACGGGGCAGGAATGTCGGGATACTCAGCCCCCGGATGGA
AAGTCAAAAGACTGCATGCTCCAGATTGTTTGTCGAGATGGGAAAACAATTAGTCTTTGTGCAGAAAGCA
CAGATGATTGCTTGGCCTGGAAATTTACACTCCAAGATTCTAGGACAAACACAGCGTATGTGGGCTCTGC
AGTCATGACCGATGAGACATCCGTGGTTTCCTCACCTCCACCATACACGGCCTATGCTGCACCGGCCCCT
GAGGAGCTGCACAGTAAAGGTCCCAAAATACACACCACCACGAGTGCAAAAACCCAGGCGGTTCTCCCAA
CGCGATGTTTTTTTCCCAGCGAATCTCCGATAAGAAGGTCTGTGCAGATTTCTGTGCTCCTACCTGCAGT
AAATATTCGTAGACGTATAAAGCTAACTTTTCCCCAGCCTGCCCGTCCGAGGGCACCAAGGAGCCTTTGC
CTTTGGCAAACATGGTTTGCAGGGAAGAGGGCGCCCAGCCTCCCCACAGCCGCCACCGCTCCGGCTCTCC
CGAGCTGCCCCTCGCCCCCGGCCCCCTCCAAGGCGCTCCCTCCCTCTCTCGCTGGCTGGCGCTCTCCTAG
CAGCGCTCCCCTCCCTCCCGAGCAGGCGCTGGCTCCGCGCTCTTTCCAGCTGTCAAAGCATCAGGCCCGG
CCGCGGCCCCATCGCCCTGGAACTCCTCCCGTGCCGGCTTGGCCTGGGGTGCCGCCGCCGCCTCCCGCAA
GGCCGCCCGCTCTCCGG

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG200155 representing NM_001031706
Red=Cloning site Green=Tags(s)

MAFVKSGWLLRQSTILKRWKKNWFDLWSDGHLIYYDDQTRQNIEDKVHMPMDCINIRTGQECRDTQPPDG
KSKDCMLQIVCRDGKTISLCAESTDDCLAWKFTLQDSRTNTAYVGSAVMTDETSVVSSPPPYTAYAAPAP
EELHSKGPKIHTTTSAKTQAVLPTRCFFPSESPIRRSVQISVLLPAVNIRRRIKLTFPQPARPRAPRSLC
LWQTWFAGKRAPSLPTAATAPALPSCPSPPAPSKALPPSLAGWRSPSSAPLPPEQALAPRSFQLSKHQAR
PRPHRPGTPPVPAWPGVPPPPPARPPALR

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001031706
ORF Size 927 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001031706.1, NP_001026876.1
RefSeq Size 1197 bp
RefSeq ORF 929 bp
Locus ID 55041
Cytogenetics 2q21.1
Summary Involved in retrograde transport of recycling endosomes.UniProtKB/Swiss-Prot Function
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"NM_001031706" in other vectors (5)
SKU Description Size Price
RC200155 PLEKHB2 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family B (evectins) member 2 (PLEKHB2), transcript variant 1 10 ug
$300.00
RC200155L1 Lenti ORF clone of Human pleckstrin homology domain containing, family B (evectins) member 2 (PLEKHB2), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC200155L2 Lenti ORF clone of Human pleckstrin homology domain containing, family B (evectins) member 2 (PLEKHB2), transcript variant 1, mGFP tagged 10 ug
$600.00
RC200155L3 Lenti ORF clone of Human pleckstrin homology domain containing, family B (evectins) member 2 (PLEKHB2), transcript variant 1, Myc-DDK-tagged 10 ug
$600.00
RC200155L4 Lenti ORF clone of Human pleckstrin homology domain containing, family B (evectins) member 2 (PLEKHB2), transcript variant 1, mGFP tagged 10 ug
$600.00

Plasmid Map for RG200155

Download sequence for

file-download TXT (FASTA format)

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Cloning scheme for RG200155
Circular map for RG200155

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