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NDC1 (NM_018087) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG200141
TMEM48 (tGFP-tagged) - Human transmembrane protein 48 (TMEM48), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
$789.00 $817.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol NDC1
Synonyms NET3; TMEM48
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG200141 representing NM_018087
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCACGGCCGTGAGCCGGCCCTGCGCCGGCAGGTCGCGGGACATACTGTGGCGCGTTTTGGGCTGGA
GGATAGTTGCAAGTATTGTTTGGTCAGTGCTATTTCTACCCATCTGCACCACAGTATTTATAATTTTCAG
CAGGATTGATTTGTTTCATCCTATACAGTGGCTGTCTGATTCTTTCAGTGACCTGTATAGTTCCTATGTA
ATCTTTTACTTCCTGCTGCTGTCAGTGGTAATAATAATAATAAGTATTTTCAATGTGGAGTTCTATGCAG
TTGTGCCTTCTATTCCTTGCTCCAGACTAGCTCTGATAGGGAAGATCATTCATCCTCAGCAACTCATGCA
CTCATTTATTCATGCTGCAATGGGAATGGTGATGGCCTGGTGTGCTGCAGTGATAACCCAGGGCCAGTAC
AGCTTTCTTGTGGTTCCCTGCACTGGTACTAACAGCTTTGATAGCCCTGCTGCGCAAACCTGCTTAAATG
AATATCATCTTTTTTTCCTACTGACTGGAGCATTTATGGGCTATAGCTATAGCCTCCTGTATTTTGTTAA
CAACATGAACTATCTTCCATTTCCCATCATACAGCAATACAAGTTCTTGCGTTTTAGGAGATCTCTGCTC
TTATTAGTTAAACACAGTTGTGTGGAATCACTGTTCCTGGTTAGAAATTTCTGCATTTTATATTATTTTC
TTGGCTATATTCCCAAAGCTTGGATTAGCACTGCTATGAACCTTCACATAGATGAGCAGGTTCATAGGCC
ACTTGACACAGTGAGTGGCCTCTTAAATCTCTCGTTACTCTACCATGTCTGGCTGTGTGGTGTCTTTCTC
CTGACGACTTGGTATGTCTCATGGATACTCTTCAAAATCTATGCCACAGAGGCTCATGTGTTTCCTGTTC
AACCACCATTTGCAGAAGGGTCAGATGAGTGCCTTCCAAAAGTGTTAAATAGCAATCCTCCCCCCATCAT
AAAGTATTTAGCCTTGCAGGACCTGATGTTGCTTTCTCAATATTCTCCTTCACGAAGACAAGAAGTTTTC
AGCCTCAGCCAACCAGGTGGACATCCCCACAATTGGACAGCCATTTCAAGGGAGTGTTTGAATCTTTTAA
ATGGTATGACTCAGAAACTGATTCTCTATCAAGAAGCTGCTGCTACGAATGGGAGAGTGTCTTCATCTTA
CCCAGTGGAACCTAAGAAATTAAATTCTCCAGAAGAAACTGCTTTTCAGACACCAAAATCTAGCCAGATG
CCTCGGCCTTCAGTGCCACCATTAGTTAAAACATCACTGTTTTCTTCAAAATTATCTACACCTGATGTTG
TGAGCCCATTTGGGACCCCATTTGGCTCTAGTGTAATGAATCGGATGGCTGGAATTTTTGATGTAAACAC
CTGCTATGGGTCACCGCAAAGTCCTCAGCTAATAAGAAGGGGGCCAAGATTGTGGACATCAGCTTCTGAT
CAGCAAATGACTGAATTTTCTAATCCTTCTCCATCTACCTCTATTAGTGCTGAGGGTAAGACAATGAGAC
AACCCAGTGTGATTTATTCATGGATTCAGAATAAACGTGAACAGATTAAGAATTTCTTGTCAAAACGGGT
GCTGATAATGTATTTTTTCAGTAAGCACCCAGAGGCCTCCATTCAGGCTGTTTTTTCAGATGCCCAAATG
CATATTTGGGCATTAGAAGGTCTGTCGCACTTAGTAGCAGCATCATTTACAGAGGATAGATTTGGAGTTG
TCCAGACGACACTACCAGCTATCCTTAATACTTTGTTGACACTGCAAGAGGCAGTCGACAAGTACTTTAA
GCTTCCTCATGCTTCCAGTAAACCACCCCGGATTTCAGGAAGCCTTGTGGACACTTCATATAAAACATTA
AGATTTGCATTCAGAGCATCACTGAAAACTGCCATCTATCGAATAACTACTACATTTGGTGAACATCTGA
ATGCTGTGCAAGCATCTGCAGAACATCAGAAAAGACTTCAACAGTTCTTGGAGTTCAAAGAA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG200141 representing NM_018087
Red=Cloning site Green=Tags(s)

MATAVSRPCAGRSRDILWRVLGWRIVASIVWSVLFLPICTTVFIIFSRIDLFHPIQWLSDSFSDLYSSYV
IFYFLLLSVVIIIISIFNVEFYAVVPSIPCSRLALIGKIIHPQQLMHSFIHAAMGMVMAWCAAVITQGQY
SFLVVPCTGTNSFDSPAAQTCLNEYHLFFLLTGAFMGYSYSLLYFVNNMNYLPFPIIQQYKFLRFRRSLL
LLVKHSCVESLFLVRNFCILYYFLGYIPKAWISTAMNLHIDEQVHRPLDTVSGLLNLSLLYHVWLCGVFL
LTTWYVSWILFKIYATEAHVFPVQPPFAEGSDECLPKVLNSNPPPIIKYLALQDLMLLSQYSPSRRQEVF
SLSQPGGHPHNWTAISRECLNLLNGMTQKLILYQEAAATNGRVSSSYPVEPKKLNSPEETAFQTPKSSQM
PRPSVPPLVKTSLFSSKLSTPDVVSPFGTPFGSSVMNRMAGIFDVNTCYGSPQSPQLIRRGPRLWTSASD
QQMTEFSNPSPSTSISAEGKTMRQPSVIYSWIQNKREQIKNFLSKRVLIMYFFSKHPEASIQAVFSDAQM
HIWALEGLSHLVAASFTEDRFGVVQTTLPAILNTLLTLQEAVDKYFKLPHASSKPPRISGSLVDTSYKTL
RFAFRASLKTAIYRITTTFGEHLNAVQASAEHQKRLQQFLEFKE

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_018087
ORF Size 2022 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_018087.3, NP_060557.2
RefSeq Size 2541 bp
RefSeq ORF 2025 bp
Locus ID 55706
UniProt ID Q9BTX1
Cytogenetics 1p32.3
Protein Families Transmembrane
Summary Component of the nuclear pore complex (NPC), which plays a key role in de novo assembly and insertion of NPC in the nuclear envelope. Required for NPC and nuclear envelope assembly, possibly by forming a link between the nuclear envelope membrane and soluble nucleoporins, thereby anchoring the NPC in the membrane.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_018087" in other vectors (6)
SKU Description Size Price
RC200141 TMEM48 (Myc-DDK-tagged)-Human transmembrane protein 48 (TMEM48), transcript variant 1 10 ug
$617.00
RC200141L1 Lenti ORF clone of Human transmembrane protein 48 (TMEM48), transcript variant 1, Myc-DDK-tagged 10 ug
$917.00
RC200141L2 Lenti ORF clone of Human transmembrane protein 48 (TMEM48), transcript variant 1, mGFP tagged 10 ug
$917.00
RC200141L3 Lenti ORF clone of Human transmembrane protein 48 (TMEM48), transcript variant 1, Myc-DDK-tagged 10 ug
$917.00
RC200141L4 Lenti ORF clone of Human transmembrane protein 48 (TMEM48), transcript variant 1, mGFP tagged 10 ug
$917.00
SC321589 TMEM48 (untagged)-Human transmembrane protein 48 (TMEM48), transcript variant 1 10 ug
$618.00

Plasmid Map for RG200141

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG200141
Circular map for RG200141

file-alt Citations

G4C2 Repeat RNA Initiates a POM121-Mediated Reduction in Specific Nucleoporins in C9orf72 ALS/FTD
Coyne, Zaepfel, Hayes et al
Neuron (2020) 107 (6), 1124-1140.e11 DOI: 10.1016/j.neuron.2020.06.027
Cited by 169
AI Summary: In this study, OriGene products pCMV6-AC-GFP-POM121, pCMV6-AC-GFP-Nup133, pCMV6-AC-GFP-Nup98, pCMV6-AC-GFP-GP210, and pCMV6-AC-GFP-NDC1 were used as plasmids to express and visualize specific nucleoporins, enabling the investigation of how G4C2 repeat RNA impacts nucleoporin levels in C9orf72 ALS/FTD neurons.
Snippet: Plasmid: pCMV6-AC-GFP-POM121 Origene Technologies Cat#RG235113 Plasmid: pCMV6-AC-GFP-Nup133 Origene Technologies Cat#RG204410 Plasmid: pCMV6-AC-GFP-Nup98 Origene Technologies Cat#RG213664 Plasmid: pCMV6-AC-GFP-GP210 Origene Technologies Cat#RG213658 Plasmid: pCMV6-AC-GFP-NDC1 Origene Technologies Cat#RG200141 Plasmid: pEGFP-POM121 Eilenberg Lab;Doucet et al., 2010;Talamas and Hetzer, 2011;Franks et al., 2016
Showing 1-1 of 1 papers.
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