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IFT122 (NM_052990) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG200119
IFT122 (tGFP-tagged) - Human intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 4
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
$789.00 $1,184.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "IFT122 or NM_052990"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol IFT122
Synonyms CED; CED1; FAP80; SPG; WDR10; WDR10p; WDR140
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG200119 representing NM_052990
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGGCCGTGTTGACGTGGAGAGATAAAGCCGAGCACTGTATAAATGACATCGCATTTAAGCCTGATG
GAACTCAACTGATTTTGGCTGCCGGAAGCAGATTACTGGTTTATGACACCTCTGATGGCACCTTACTTCA
GCCCCTCAAGGGACACAAAGACACTGTGTACTGTGTGGCATATGCGAAGGATGGGTTGTGGTCTCCTGAA
CAGAAGTCTGTCTCCAAACACAAATCAAGCAGCAAGATCATCTGCTGCAGCTGGACAAATGATGGTCAGT
ACCTGGCGCTGGGGATGTTCAATGGGATCATCAGCATACGGAACAAAAATGGCGAGGAGAAAGTAAAGAT
CGAGCGGCCGGGGGGCTCCCTCTCGCCAATATGGTCCATCTGCTGGAACCCTTCAAGAGAGGAACGTAAT
GACATCCTGGCTGTGGCTGACTGGGGACAGAAAGTTTCCTTCTACCAGCTGAGTGGAAAACAGATTGGAA
AGGATCGGGCACTGAACTTTGACCCCTGCTGCATCAGCTACTTTACTAAAGGCGAGTACATTTTGCTGGG
GGGTTCAGACAAGCAAGTATCTCTTTTCACCAAGGATGGAGTGCGGCTTGGGACTGTTGGGGAGCAGAAC
TCCTGGGTGTGGACGTGTCAAGCGAAACCGGATTCCAACTATGTGGTGGTCGGCTGCCAGGACGGCACCA
TTTCCTTCTACCAGCTTATTTTCAGCACAGTCCATGGGCTTTACAAGGACCGCTATGCCTACAGGGATAG
CATGACTGACGTCATTGTGCAGCACCTGATCACTGAGCAGAAAGTTCGGATTAAATGCAAAGAGCTTGTC
AAGAAGATTGCCATCTACAGAAATCGATTGGCTATCCAACTGCCAGAGAAAATCCTCATCTATGAGTTGT
ATTCAGAGGACTTATCAGACATGCATTACCGGGTAAAGGAGAAGATTATCAAGAAGTTTGAGTGCAACCT
CCTGGTGGTGTGTGCCAATCACATCATCCTGTGCCAGGAGAAACGGCTGCAGTGCCTGTCCTTCAGCGGA
GTGAAGGAGCGGGAGTGGCAGATGGAGTCTCTCATTCGTTACATCAAGGTGATCGGTGGCCCTCCTGGAA
GAGAAGGCCTCTTAGTGGGGCTGAAGAATGGACAGATCCTGAAGATCTTCGTGGACAATCTCTTTGCTAT
CGTCCTGCTGAAGCAGGCCACAGCTGTGCGCTGCTTGGACATGAGTGCCTCCCGTAAGAAGCTGGCCGTG
GTAGATGAAAATGACACTTGCCTGGTGTATGACATCGACACCAAGGAGCTGCTTTTTCAGGAACCAAACG
CCAACAGTGTAGCTTGGAACACCCAGTGTGAGGACATGCTCTGCTTCTCGGGAGGAGGCTACCTCAACAT
CAAAGCCAGCACCTTCCCTGTGCACCGGCAGAAGCTGCAGGGCTTTGTGGTCGGCTACAATGGCTCCAAG
ATCTTCTGCCTCCATGTCTTCTCCATTTCTGCCGTGGAGGTGCCGCAGTCCGCTCCCATGTACCAGTACC
TGGATAGGAAACTGTTCAAGGAAGCCTACCAGATTGCTTGCTTGGGTGTCACAGACACTGATTGGCGTGA
ACTGGCCATGGAAGCGCTAGAAGGTTTAGATTTTGAAACTGCAAAGAAGGCCTTCATCAGAGTACAAGAC
CTCCGATATTTAGAGCTCATCAGCAGCATTGAGGAGAGGAAGAAGCGGGGAGAGACCAACAATGACCTGT
TTCTGGCAGATGTGTTTTCCTACCAGGGGAAGTTCCATGAGGCCGCCAAACTGTACAAGAGGAGTGGGCA
CGAGAACCTCGCGCTTGAAATGTACACCGACCTCTGCATGTTTGAGTATGCCAAGGATTTCCTTGGATCT
GGAGACCCCAAAGAAACAAAGATGCTAATCACCAAACAGGCTGACTGGGCCAGAAATATCAAGGAGCCCA
AAGCCGCCGTGGAGATGTACATCTCAGCAGGAGAGCACGTCAAGGCCATCGAGATCTGTGGTGACCATGG
CTGGGTTGACATGTTGATCGACATCGCCCGCAAACTGGACAAGGCTGAGCGCGAGCCCCTGCTGCTGTGC
GCTACCTACCTCAAGAAGCTGGACAGCCCTGGCTATGCTGCTGAGACCTACCTGAAGATGGGTGACCTCA
AGTCCCTGGTGCAGCTGCACGTGGAGACCCAGCGCTGGGATGAGGCCTTTGCTTTGGGTGAGAAGCATCC
TGAGTTTAAGGATGACATCTACATGCCGTATGCTCAGTGGCTAGCAGAGAACGATCGCTTTGAGGAAGCC
CAGAAAGCGTTCCACAAGGCTGGGCGACAGAGAGAAGCGGTCCAGGTGCTGGAGCAGCTCACAAACAATG
CCGTGGCGGAGAGCAGGTTTAATGATGCTGCCTATTATTACTGGATGCTGTCCATGCAGTGCCTCGATAT
AGCTCAAGCAGATCCTGCCCAGAAGGACACAATGCTTGGCAAGTTCTACCACTTCCAGCGTTTGGCAGAG
CTGTACCATGGTTACCATGCCATCCATCGCCACACGGAAGATCCGTTCAGTGTCCATCGTCCTGAAACTC
TTTTCAACATCTCCAGGTTCCTGCTGCACAGCCTGCCCAAGGACACCCCCTCGGGCATCTCTAAAGTGAA
AATACTCTTCACCTTGGCCAAGCAGAGCAAGGCCCTCGGTGCCTACAGGCTGGCCCGGCACGCCTATGAC
AAGCTGCGTGGCCTGTACATCCCTGCCAGATTCCAAAAGTCCATTGAGCTGGGTACCCTGACCATCCGCG
CCAAGCCCTTCCACGACAGTGAGGAGTTGGTGCCCTTGTGCTACCGCTGCTCCACCAACAACCCGCTGCT
CAACAACCTGGGCAACGTCTGCATCAACTGCCGCCAGCCCTTCATCTTCTCCGCCTCTTCCTACGACGTG
CTACACCTGGTTGAGTTCTACCTGGAGGAAGGGATCACTGATGAAGAAGCCATCTCCCTCATCGACCTGG
AGGTGCTGAGACCCAAGCGGGATGACAGACAGCTAGAGATTGCAAACAACAGCTCCCAGATTCTGCGGCT
AGTGGAGACCAAGGACTCCATCGGAGATGAGGACCCGTTCACAGCTAAGCTGAGCTTTGAGCAAGGTGGC
TCAGAGTTCGTGCCAGTGGTGGTGAGCCGGCTGGTGCTGCGCTCCATGAGCCGCCGGGATGTCCTCATCA
AGCGATGGCCCCCACCCCTGAGGTGGCAATACTTCCGCTCACTGCTGCCTGACGCCTCCATTACCATGTG
CCCCTCCTGCTTCCAGATGTTCCATTCTGAGGACTATGAGTTGCTGGTGCTTCAGCATGGCTGCTGCCCC
TACTGCCGCAGGTGCAAGGATGACCCTGGCCCA

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG200119 representing NM_052990
Red=Cloning site Green=Tags(s)

MRAVLTWRDKAEHCINDIAFKPDGTQLILAAGSRLLVYDTSDGTLLQPLKGHKDTVYCVAYAKDGLWSPE
QKSVSKHKSSSKIICCSWTNDGQYLALGMFNGIISIRNKNGEEKVKIERPGGSLSPIWSICWNPSREERN
DILAVADWGQKVSFYQLSGKQIGKDRALNFDPCCISYFTKGEYILLGGSDKQVSLFTKDGVRLGTVGEQN
SWVWTCQAKPDSNYVVVGCQDGTISFYQLIFSTVHGLYKDRYAYRDSMTDVIVQHLITEQKVRIKCKELV
KKIAIYRNRLAIQLPEKILIYELYSEDLSDMHYRVKEKIIKKFECNLLVVCANHIILCQEKRLQCLSFSG
VKEREWQMESLIRYIKVIGGPPGREGLLVGLKNGQILKIFVDNLFAIVLLKQATAVRCLDMSASRKKLAV
VDENDTCLVYDIDTKELLFQEPNANSVAWNTQCEDMLCFSGGGYLNIKASTFPVHRQKLQGFVVGYNGSK
IFCLHVFSISAVEVPQSAPMYQYLDRKLFKEAYQIACLGVTDTDWRELAMEALEGLDFETAKKAFIRVQD
LRYLELISSIEERKKRGETNNDLFLADVFSYQGKFHEAAKLYKRSGHENLALEMYTDLCMFEYAKDFLGS
GDPKETKMLITKQADWARNIKEPKAAVEMYISAGEHVKAIEICGDHGWVDMLIDIARKLDKAEREPLLLC
ATYLKKLDSPGYAAETYLKMGDLKSLVQLHVETQRWDEAFALGEKHPEFKDDIYMPYAQWLAENDRFEEA
QKAFHKAGRQREAVQVLEQLTNNAVAESRFNDAAYYYWMLSMQCLDIAQADPAQKDTMLGKFYHFQRLAE
LYHGYHAIHRHTEDPFSVHRPETLFNISRFLLHSLPKDTPSGISKVKILFTLAKQSKALGAYRLARHAYD
KLRGLYIPARFQKSIELGTLTIRAKPFHDSEELVPLCYRCSTNNPLLNNLGNVCINCRQPFIFSASSYDV
LHLVEFYLEEGITDEEAISLIDLEVLRPKRDDRQLEIANNSSQILRLVETKDSIGDEDPFTAKLSFEQGG
SEFVPVVVSRLVLRSMSRRDVLIKRWPPPLRWQYFRSLLPDASITMCPSCFQMFHSEDYELLVLQHGCCP
YCRRCKDDPGP

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_052990
ORF Size 3393 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_052990.1, NP_443716.1
RefSeq Size 3717 bp
RefSeq ORF 3396 bp
Locus ID 55764
UniProt ID Q9HBG6
Cytogenetics 3q21.3-q22.1
Domains WD40
Summary This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This cytoplasmic protein contains seven WD repeats and an AF-2 domain which function by recruiting coregulatory molecules and in transcriptional activation. Mutations in this gene cause cranioectodermal dysplasia-1. A related pseudogene is located on chromosome 3. Alternative splicing results in multiple transcript variants encoding different isoforms. provided by RefSeq, Jul 2013
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"NM_052990" in other vectors (5)
SKU Description Size Price
RC200119 IFT122 (Myc-DDK-tagged)-Human intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 4 10 ug
$984.00
RC200119L3 Lenti ORF clone of Human intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 4, Myc-DDK-tagged 10 ug
$1,284.00
RC200119L4 Lenti ORF clone of Human intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 4, mGFP tagged 10 ug
$1,284.00
SC110527 IFT122 (untagged)-Human intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 4 10 ug
$985.00
SC320617 IFT122 (untagged)-Human intraflagellar transport 122 homolog (Chlamydomonas) (IFT122), transcript variant 4 10 ug
$985.00

Plasmid Map for RG200119

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG200119
Circular map for RG200119

file-alt Citations

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