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SKIP (INPP5K) (NM_016532) Human Tagged ORF Clone

  • TrueORF®
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SKU
RG200092
INPP5K (tGFP-tagged) - Human inositol polyphosphate-5-phosphatase K (INPP5K), transcript variant 1
ORF Plasmid:
tGFP DDK
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$489.00 $657.00
In Stock*
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Other products for "SKIP or NM_016532"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SKIP
Synonyms MDCCAID; PPS; SKIP
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RG200092 representing NM_016532
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCTCGCGGAAGCTGAGCGGGCCGAAAGGCAGGAGGCTCAGCATACACGTCGTGACTTGGAACGTGG
CTTCGGCAGCGCCCCCTCTAGATCTCAGTGACCTGCTTCAGCTGAACAACCGGAACCTCAATCTTGACAT
ATATGTTATTGGTTTGCAGGAATTGAACTCTGGGATCATAAGCCTCCTTTCCGATGCTGCCTTTAATGAC
TCGTGGAGCAGTTTCCTCATGGATGTGCTTTCCCCTCTGAGCTTCATCAAGGTCTCCCATGTCCGTATGC
AGGGGATCCTCTTACTGGTCTTTGCCAAGTATCAGCATTTGCCCTATATCCAGATTCTGTCTACTAAATC
CACCCCCACTGGCCTGTTTGGGTACTGGGGGAACAAAGGTGGAGTCAACATCTGCCTGAAGCTTTATGGC
TACTATGTCAGCATCATCAACTGCCACCTGCCTCCCCACATTTCCAACAATTACCAGCGGCTGGAGCACT
TTGACCGGATCCTGGAGATGCAGAATTGTGAGGGGCGAGACATCCCAAACATCCTGGACCACGACCTCAT
TATCTGGTTTGGAGACATGAACTTTCGGATCGAGGACTTTGGGTTGCACTTTGTTCGGGAATCCATTAAA
AATCGGTGCTACGGTGGCCTGTGGGAGAAGGACCAGCTCAGCATTGCCAAGAAACATGACCCGCTGCTCC
GGGAGTTCCAGGAGGGCCGCCTACTCTTCCCGCCCACCTACAAGTTTGATAGGAACTCCAACGACTATGA
CACCAGTGAGAAAAAACGCAAGCCTGCATGGACCGATCGCATCCTGTGGAGGCTGAAGCGGCAGCCCTGT
GCTGGCCCCGACACTCCCATACCGCCGGCGTCACACTTCTCCTTGTCTCTGAGGGGCTACAGCAGCCACA
TGACGTACGGCATCAGCGACCACAAGCCTGTCTCCGGCACGTTCGACTTGGAGCTGAAGCCATTGGTGTC
TGCTCCGCTGATCGTCCTGATGCCCGAGGACCTGTGGACCGTGGAAAATGACATGATGGTCAGCTACTCT
TCAACCTCGGACTTCCCCAGCAGCCCGTGGGACTGGATTGGACTGTACAAGGTGGGGCTGCGGGACGTTA
ATGACTACGTGTCCTATGCCTGGGTCGGGGACAGCAAGGTCTCCTGCAGCGACAACCTGAACCAGGTTTA
CATCGACATCAGCAATATCCCTACCACTGAAGATGAGTTTCTCCTCTGTTACTACAGCAACAGTCTGCGT
TCTGTGGTGGGGATAAGCAGACCCTTCCAGATCCCGCCTGGCTCCTTGAGGGAGGACCCACTGGGTGAAG
CACAGCCACAGATC

ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
Protein Sequence
>RG200092 representing NM_016532
Red=Cloning site Green=Tags(s)

MSSRKLSGPKGRRLSIHVVTWNVASAAPPLDLSDLLQLNNRNLNLDIYVIGLQELNSGIISLLSDAAFND
SWSSFLMDVLSPLSFIKVSHVRMQGILLLVFAKYQHLPYIQILSTKSTPTGLFGYWGNKGGVNICLKLYG
YYVSIINCHLPPHISNNYQRLEHFDRILEMQNCEGRDIPNILDHDLIIWFGDMNFRIEDFGLHFVRESIK
NRCYGGLWEKDQLSIAKKHDPLLREFQEGRLLFPPTYKFDRNSNDYDTSEKKRKPAWTDRILWRLKRQPC
AGPDTPIPPASHFSLSLRGYSSHMTYGISDHKPVSGTFDLELKPLVSAPLIVLMPEDLWTVENDMMVSYS
STSDFPSSPWDWIGLYKVGLRDVNDYVSYAWVGDSKVSCSDNLNQVYIDISNIPTTEDEFLLCYYSNSLR
SVVGISRPFQIPPGSLREDPLGEAQPQI

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_016532
ORF Size 1344 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_016532.4
RefSeq Size 2773 bp
RefSeq ORF 1347 bp
Locus ID 51763
UniProt ID Q9BT40
Cytogenetics 17p13.3
Domains Exo_endo_phos, IPPc
Protein Families Druggable Genome, Phosphatase
Protein Pathways Inositol phosphate metabolism, Insulin signaling pathway, Metabolic pathways, Phosphatidylinositol signaling system
Summary This gene encodes a protein with 5-phosphatase activity toward polyphosphate inositol. The protein localizes to the cytosol in regions lacking actin stress fibers. It is thought that this protein may negatively regulate the actin cytoskeleton. Alternatively spliced transcript variants encoding different isoforms have been identified. provided by RefSeq, Oct 2008
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Documents
Resources
"NM_016532" in other vectors (6)
SKU Description Size Price
RC200092 INPP5K (Myc-DDK-tagged)-Human inositol polyphosphate-5-phosphatase K (INPP5K), transcript variant 1 10 ug
$457.00
RC200092L1 Lenti ORF clone of Human inositol polyphosphate-5-phosphatase K (INPP5K), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC200092L2 Lenti ORF clone of Human inositol polyphosphate-5-phosphatase K (INPP5K), transcript variant 1, mGFP tagged 10 ug
$757.00
RC200092L3 Lenti ORF clone of Human inositol polyphosphate-5-phosphatase K (INPP5K), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC200092L4 Lenti ORF clone of Human inositol polyphosphate-5-phosphatase K (INPP5K), transcript variant 1, mGFP tagged 10 ug
$757.00
SC321576 INPP5K (untagged)-Human inositol polyphosphate-5-phosphatase K (INPP5K), transcript variant 1 10 ug
$457.00

Plasmid Map for RG200092

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RG200092
Circular map for RG200092

file-alt Citations

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