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C6orf70 (ERMARD) (NM_001278532) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC238870
ERMARD (myc-DDK-tagged) - Human ER membrane-associated RNA degradation (ERMARD), transcript variant 3
  $565.00
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol C6orf70
Synonyms C6orf70; dJ266L20.3; PVNH6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC238870 representing NM_001278532
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAACTGACATCGTGTCTAGAACGAGCCTTGGGTGATGTATTTTTACTGATTGGGAAGGAATGCCCCT
TTCTTTTAAGAGATCTGCTTTCATCTGAGGAGCTTGCTCAAGTCTTCAGTCAGTCTGTGATGAATGTGCT
AAAAGTCTTCGTTGGCTCTCCGTGTGGTCTCAACCTGCGTAACGTCTTATGGCATGGGTTTGCGTCACCT
GAAGAAATTCCTCCAAAATACTGTTCAATGATGATACTGTTGACGGCAGGATTGGGTCAGTTACTGAAGA
GTTACCTTCAAAACACTAAACTTACATTGGCACATCGCTCTTTCATATCTCTTACAAACCTCGAGGATTT
GATTGTTTTTCCTGATGTTACTTATGAGGTGCTTTCAGTATTAGAAGAAGTGATGATGAAATCTGCTTTT
ATATTAAAAATCATGTTACCATATTGGGAAGTTGCACTGGTCAAGTTCAAGTCACACAGGTTTGCTGACT
GCGCCATATTGTTGCTGACACAACTGGAGACTGGACTTAGGAATGTTTTTGCCACACTTAACAGATGTCC
AAAAAGACTCCTGACTGCTGAGTCAACAGCTCTTTATACCACCTTTGATCAAATATTGGCAAAACACTTG
AATGATGGTAAAATCAATCAGCTTCCTCTTTTCCTTGGAGAGCCTGCTATGGAATTTCTCTGGGATTTCC
TGAACCATCAGGAGGGTCCCCGCATAAGAGATCATTTAAGCCACGGGGAGATCAACTTACATGAATTTTC
AAAAGAAACAACTAATCAGTTGCTTGCATTTTCTCTTGTACTGCTACTCAGATTCGTTGATGACTGTCTG
CTATCAGTTTTTAAGGAAAAATCAGCCGTAGAATTGTTGATTAGTCTTGCAGAAGGCTATAGTTCTCGCT
GTCATCCGGTTTTTCAGCTTAAAAAACAGGTGCTGAGCTGTGAGGAGAGCATCAGGGTTTGGGCTCTGCT
GCCTTTCCCCGAAGAACTCACTCGGCAAGCCGTCAGATTAGAAGATAATTCTGAAACAAATGCCTGCCAC
TCTTTGATTACAAAAATGACGGATGAGCTGTATCACCATATGCCTGAGAATCGTTGTGTGTTAAAGGACT
TGGATCGTCTTCCTACTGAGACGTGGCCCCAGCTTCTCCGTGAGCTCTGCAGCACACCTGTTCCCACCCT
GTTCTGCCCCAGGATTGTGCTGGAAGTGCTGGTTGTGCTCCGAAGCATCAGCGAACAGTGCCGCCGTGTG
TCCAGCCAGGTCACCGTTGCCTCAGAGCTGAGACACAGGCAGTGGGTGGAAAGGACGCTGCGGTCTCGCC
AGCGGCAGAACTACCTGCGTATGTGGAGTAGTATCAGACTACTGTCCCCTGTGCTCAGCCTGATACTGTT
ACTCATTGCGCTGGAGTTGGTCAACATTCATGCTGTTTGTGGGAAGAATGCGCATGAGTATCAGCAGTAC
CTAAAGTTTGTAAAGTCGATCTTGCAGTACACGGAGAACCTGGTGGCTTACACCAGTTACGAAAAGAACA
AGTGGAATGAAACTATCAATCTTACACATACAGCTTTGTTGAAAATGTGGACTTTTAGTGAGAAGAAACA
AATGTTAATACATTTAGCCAAGAAATCCACAAGTAAAGTACTCTTA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC238870 representing NM_001278532
Red=Cloning site Green=Tags(s)

MKLTSCLERALGDVFLLIGKECPFLLRDLLSSEELAQVFSQSVMNVLKVFVGSPCGLNLRNVLWHGFASP
EEIPPKYCSMMILLTAGLGQLLKSYLQNTKLTLAHRSFISLTNLEDLIVFPDVTYEVLSVLEEVMMKSAF
ILKIMLPYWEVALVKFKSHRFADCAILLLTQLETGLRNVFATLNRCPKRLLTAESTALYTTFDQILAKHL
NDGKINQLPLFLGEPAMEFLWDFLNHQEGPRIRDHLSHGEINLHEFSKETTNQLLAFSLVLLLRFVDDCL
LSVFKEKSAVELLISLAEGYSSRCHPVFQLKKQVLSCEESIRVWALLPFPEELTRQAVRLEDNSETNACH
SLITKMTDELYHHMPENRCVLKDLDRLPTETWPQLLRELCSTPVPTLFCPRIVLEVLVVLRSISEQCRRV
SSQVTVASELRHRQWVERTLRSRQRQNYLRMWSSIRLLSPVLSLILLLIALELVNIHAVCGKNAHEYQQY
LKFVKSILQYTENLVAYTSYEKNKWNETINLTHTALLKMWTFSEKKQMLIHLAKKSTSKVLL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001278532
ORF Size 1656 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001278532.2
RefSeq Size 1975 bp
RefSeq ORF 1659 bp
Locus ID 55780
UniProt ID Q5T6L9
Cytogenetics 6q27
Protein Families Transmembrane
MW 64 kDa
Summary The protein encoded by this gene contains 2 transmembrane domains near the C-terminus and is localized in the endoplasmic reticulum. Knockout of this gene in developing rat brain showed that it may be involved in neuronal migration. Mutations in this gene are associated with periventricular nodular heterotopia-6 (PVNH6). Alternatively spliced transcript variants have been found for this gene. provided by RefSeq, Dec 2013
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Documents
Resources

Plasmid Map for RC238870

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC238870
Circular map for RC238870

file-alt Citations

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