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LRRC74B (NM_001291006) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC237937
LRRC74B (myc-DDK-tagged) - Human leucine rich repeat containing 74B (LRRC74B), transcript variant 1
ORF Plasmid:
DDK tGFP
  $503.00
2 Weeks*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LRRC74B
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC237937 representing NM_001291006
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGGGTTCCTGTGAGAGGTCTGGGGAGGATGAAGAGCAGAAAGAAGAGGCTATGGTGGCCTGTGGGC
GTCTTTCAGGGGTCCCCGAGGCCGAGCAGGGTCCCGAGGCCAATTGGGACTCCGACCTGGAGACGGAAGG
CACCGATGGGCTTGGAGAACTGGTCAGGGACACACTGTACCTGAGGTCTTGCCGGGCCCATAGTGTTGTG
CCCATCTCCTGCTTTCTGCGCCAAGGGAGCGCCCAAGAGCTGAACCTCCGGCACCGTGGCCTGGGGCCCC
AGGGCGCCCGGGCTCTGGCTTCCTCATTGAGCTCCAATCCATATGTCAAGCGGCTGGACCTTCGAGACAA
TGGGCTCTGTGGGGCAGGTGCAGAGGCCCTGGCAGGTGCCCTGAGCAAAAGCAGCAGCATCCATGATGTG
GACCTGTCGGAGAACCAGCTGGGAGTGGCAGGAGCCCAGGCCCTCTGTGCCGCCCTCACAGTGAACCAGG
CCATGCGGAAGATGCAGCTGTCAGGGAATGGCCTGGAGGAGCAGGCGGCCCAGCACCTTGCCGAACTCCT
GCTGGCCCACACAGACCTGAAGTCCCTGGACCTGAGCTACAACCAGCTGAATGACCAAGCAGGGGAGACA
CTTGGACCAGCCCTGGCAGAAAACACAGGACTCACCGAGCTTAACGTGAGCTGGAATCACCTCCGGGGCC
CAGGAGCTGTGGCATTTGCCAGGGGACTGGAGGCAAACATCTTCCTTAAAGTTCTAGACATCTCATACAA
TGGCTTTGGGGATCCTGGAGCCTCTGCGGTGGGTGAGGCTCTGAAGGCCAACAACGTGTTGGAGGAACTC
AACATGAGTAACAACCGCATCTCTGCGATGGGAGCCCTGAGCCTGGGCCTGGGCCTCCGGGTCAACCAGA
CGCTGAGGATTCTTGTAGTGTCCAGGAATCCCATGCGAAGTGAAGGCTGCTTTGGTCTCCTCAAGTCTGT
CCAGGACAATCCAGCCTCTGCCCTGGAACTGCTGGATTTCTCAGATATCCAGGTGAACGCAGAGTTTGAT
GGCCTTGCTAGCTCAGTGAGGGGAATTCTTCCAGAACTCTGCATAAAGACTGGCGCTTGCAGAGTGGAGT
ATAAAAAGGAGTTGCTGCCAGTCTTCAGATCAGCCCTGCCAGCGTCTGTCCCTAAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC237937 representing NM_001291006
Red=Cloning site Green=Tags(s)

MRGSCERSGEDEEQKEEAMVACGRLSGVPEAEQGPEANWDSDLETEGTDGLGELVRDTLYLRSCRAHSVV
PISCFLRQGSAQELNLRHRGLGPQGARALASSLSSNPYVKRLDLRDNGLCGAGAEALAGALSKSSSIHDV
DLSENQLGVAGAQALCAALTVNQAMRKMQLSGNGLEEQAAQHLAELLLAHTDLKSLDLSYNQLNDQAGET
LGPALAENTGLTELNVSWNHLRGPGAVAFARGLEANIFLKVLDISYNGFGDPGASAVGEALKANNVLEEL
NMSNNRISAMGALSLGLGLRVNQTLRILVVSRNPMRSEGCFGLLKSVQDNPASALELLDFSDIQVNAEFD
GLASSVRGILPELCIKTGACRVEYKKELLPVFRSALPASVPK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001291006
ORF Size 1176 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001291006.2
RefSeq Size 3700 bp
RefSeq ORF 1179 bp
Locus ID 400891
UniProt ID Q6ZQY2
Cytogenetics 22q11.21
MW 42.1 kDa
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"NM_001291006" in other vectors (2)
SKU Description Size Price
RG237937 LRRC74B (tGFP-tagged) - Human leucine rich repeat containing 74B (LRRC74B), transcript variant 1 10 ug
$703.00
SC335831 LRRC74B (untagged) - Human leucine rich repeat containing 74B (LRRC74B), transcript variant 1 10 ug
$503.00

Plasmid Map for RC237937

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC237937
Circular map for RC237937

file-alt Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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