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SMIM23 (NM_001289970) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC236257
SMIM23 (myc-DDK-tagged) - Human small integral membrane protein 23 (SMIM23)
ORF Plasmid:
DDK tGFP
  $330.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol SMIM23
Synonyms C5orf50
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC236257 representing NM_001289970
Blue=ORF Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATT
CGTCGACTGGATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC

ATGGCAACCCAGCAAGTGGACAGCAGAAGGCAGGTGGCAGCAGAGCAGGTGGCAGCCCAG
CTGCTTGAACGGAGAAGGGGCAGCCACTGTGATGACGAGAAGCAGACGCTGTTGGCATTG
CTGATCTTGGTGCTGTACTTGAGCACAGAGATATGGGGAAGCAGTTGGGAGGTGTCAGAA
AGGATCAGAGAATGTAACTACTACCAGAATCTTGCAGTTCCCCAGGGGCTTGAATATCAG
ACCAACGAGCCCTCAGAAGAACCGATAAAGACCATCAGGAACTGGCTGAAGGAGAAGTTG
CATGTCTTCTCGGAGAAGTTAGAGGAAGAGGTGCAGCAGCTGGAGCAGCTAGCGTGGGAC
CTGGAACTGTGGCTGGATGCTCTTCTGGGAGAGCCACACCAGGAGGAGCACTGCTCCACA
TATAAAAGTCACTTGTGGGAGTGGGCCTGGGCCCTGGGGAGAGAGCACAAAGGTGGGGAG
GGGTTGCTAGAGATTTCTCTAAGCGGGGCAGAGCTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATTACAAGGAT
GACGACGATAAGGTTTAAA
Protein Sequence
>RC236257 protein sequence
Blue=ORF Red=Cloning site Green=Tag(s)

MATQQVDSRRQVAAEQVAAQLLERRRGSHCDDEKQTLLALLILVLYLSTEIWGSSWEVSE
RIRECNYYQNLAVPQGLEYQTNEPSEEPIKTIRNWLKEKLHVFSEKLEEEVQQLEQLAWD
LELWLDALLGEPHQEEHCSTYKSHLWEWAWALGREHKGGEGLLEISLSGAEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001289970
ORF Size 516 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001289970.2
RefSeq Size 519 bp
RefSeq ORF 519 bp
Locus ID 644994
UniProt ID A6NLE4
Cytogenetics 5q35.1
MW 20.5 kDa
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"NM_001289970" in other vectors (2)
SKU Description Size Price
RG236257 SMIM23 (tGFP-tagged) - Human small integral membrane protein 23 (SMIM23) 10 ug
$530.00
SC334151 SMIM23 (untagged) - Human small integral membrane protein 23 (SMIM23) 10 ug
$330.00

Plasmid Map for RC236257

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC236257
Circular map for RC236257

file-alt Citations

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