Complement C4A (C4A) (NM_001252204) Human Tagged ORF Clone
- TrueORF®
SKU
RC235329
C4A (Myc-DDK tagged) - Homo sapiens complement component 4A (Rodgers blood group) (C4A), transcript variant 2
$2,283.00
2 Weeks*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | Complement C4A |
| Synonyms | C4; C4A2; C4A3; C4A4; C4A6; C4AD; C4S; CO4; CPAMD2; RG |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001252204 |
| ORF Size | 5094 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001252204.1, NP_001239133.1 |
| RefSeq Size | 5288 bp |
| RefSeq ORF | 5097 bp |
| Locus ID | 720 |
| UniProt ID | P0C0L4 |
| Cytogenetics | 6p21.33 |
| Protein Pathways | Complement and coagulation cascades, Systemic lupus erythematosus |
| MW | 188.2 kDa |
| Summary | This gene encodes the acidic form of complement factor 4, part of the classical activation pathway. The protein is expressed as a single chain precursor which is proteolytically cleaved into a trimer of alpha, beta, and gamma chains prior to secretion. The trimer provides a surface for interaction between the antigen-antibody complex and other complement components. The alpha chain is cleaved to release C4 anaphylatoxin, an antimicrobial peptide and a mediator of local inflammation. Deficiency of this protein is associated with systemic lupus erythematosus and type I diabetes mellitus. This gene localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. Varying haplotypes of this gene cluster exist, such that individuals may have 1, 2, or 3 copies of this gene. Two transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Nov 2014 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001252204" in other vectors (2)
Citations
Increased expression of schizophrenia-associated gene C4 leads to hypoconnectivity of prefrontal cortex and reduced social interaction
Comer, Jinadasa, Sriram et al
PLoS Biol (2020) 18 (1), e3000604 DOI: 10.1371/journal.pbio.3000604
Comer, Jinadasa, Sriram et al
PLoS Biol (2020) 18 (1), e3000604 DOI: 10.1371/journal.pbio.3000604
Cited by 186
AI Summary: OriGene products contributed to this study by providing the C4 overexpression plasmid (Cat# MR207883) used to increase C4 expression in mouse prefrontal cortex neurons, enabling the investigation of C4’s effects on neural connectivity and behavior.
Increased expression of schizophrenia-associated gene C4 leads to hypoconnectivity of prefrontal cortex and reduced social interaction
Comer, Jinadasa, Kretsge et al
bioRxiv (2019)
Comer, Jinadasa, Kretsge et al
bioRxiv (2019)
Showing 1-2 of 2 papers.
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
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