NOXA1 (NM_001256067) Human Tagged ORF Clone
- TrueORF®
SKU
RC234211
NOXA1 (Myc-DDK tagged) - Homo sapiens NADPH oxidase activator 1 (NOXA1), transcript variant 2
$686.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | NOXA1 |
| Synonyms | NY-CO-31; p51NOX; SDCCAG31 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001256067 |
| ORF Size | 1428 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001256067.2 |
| RefSeq Size | 1622 bp |
| RefSeq ORF | 1431 bp |
| Locus ID | 10811 |
| UniProt ID | Q86UR1 |
| Cytogenetics | 9q34.3 |
| MW | 51.4 kDa |
| Summary | This gene encodes a protein which activates NADPH oxidases, enzymes which catalyze a reaction generating reactive oxygen species. The encoded protein contains four N-terminal tetratricopeptide domains and a C-terminal Src homology 3 domain. Interaction between the encoded protein and proteins in the oxidase regulatory complex occur via the tetratricopeptide domains. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Dec 2011 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Citations
CRISPR/Cas9-mediated knockout of p22phox leads to loss of Nox1 and Nox4, but not Nox5 activity
Prior, Leisegang, Josipovic et al
Redox Biol (2016) 9, 287-295 DOI: 10.1016/j.redox.2016.08.013
Prior, Leisegang, Josipovic et al
Redox Biol (2016) 9, 287-295 DOI: 10.1016/j.redox.2016.08.013
Cited by 50
AI Summary: In this study, OriGene products—including the human CYBA cDNA clone (RC204232) and rat Cyba cDNA clone (RG221865)—were used to rescue p22phox expression in CRISPR/Cas9 knockout cells, enabling the authors to demonstrate the essential role of native p22phox in Nox1 and Nox4 activity but not in Nox5 function.
Showing 1-1 of 1 papers.
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