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APC16 (ANAPC16) (NM_001242547) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC231664
ANAPC16 (Myc-DDK tagged) - Homo sapiens anaphase promoting complex subunit 16 (ANAPC16), transcript variant 3
ORF Plasmid:
DDK tGFP
AAV Particle:
DDK
  $289.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol APC16
Synonyms APC16; bA570G20.3; C10orf104; CENP-27; MSAG
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC231664 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTGCTTCATCATCATCCTCCTCAGCTGGTGGGGTCAGTGGAAGTTCTGTCACTGGATCTGGTTTCA
GTGTCTCAGACCTTGCCCCACCACGGAAAGCCCTTTTCACCTACCCCAAAGGAGCTGGAGAGATGTTAGA
AGATGGCTCTGAGAGATTCCTCTGCGAATCTGTTTTTAGCTATCAAGTGGCATCCACGCTTAAACAGGTG
AAACATGATCAGCAAGTTGCTCGGATGGAAAAACTAGCTGGTTTGGTAGAAGAGCTGGAGGCTGACGAGT
GGCGGTTTAAGCCCATCGAGCAGCTGCTGGGATTCACCCCCTCTTCAGGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC231664 protein sequence
Red=Cloning site Green=Tags(s)

MAASSSSSSAGGVSGSSVTGSGFSVSDLAPPRKALFTYPKGAGEMLEDGSERFLCESVFSYQVASTLKQV
KHDQQVARMEKLAGLVEELEADEWRFKPIEQLLGFTPSSG

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001242547
ORF Size 330 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001242547.2
RefSeq Size 3186 bp
RefSeq ORF 333 bp
Locus ID 119504
UniProt ID Q96DE5
Cytogenetics 10q22.1
MW 11.7 kDa
Summary Component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins: it mainly mediates the formation of 'Lys-11'-linked polyubiquitin chains and, to a lower extent, the formation of 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains.UniProtKB/Swiss-Prot Function
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Documents
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"NM_001242547" in other vectors (2)
SKU Description Size Price
RG231664 ANAPC16 (tGFP-tagged) - Homo sapiens anaphase promoting complex subunit 16 (ANAPC16), transcript variant 3 10 ug
$350.00
SC329987 ANAPC16 (untagged) - Homo sapiens anaphase promoting complex subunit 16 (ANAPC16), transcript variant 3 10 ug
$165.00

Plasmid Map for RC231664

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC231664
Circular map for RC231664

file-alt Citations

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