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RING finger protein unkempt like (UNKL) (NM_001193388) Human Tagged ORF Clone

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SKU
RC231370
UNKL (Myc-DDK-tagged)-Human unkempt homolog (Drosophila)-like (UNKL), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $623.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RING finger protein unkempt like
Synonyms C16orf28; ZC3H5L; ZC3HDC5L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC231370 representing NM_001193388
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCCGTCGGTTTCGAAAGCGGCGGCAGCGGCGCTGAGCGGGTCCCCCCCGCAGACTGAGAAGCCGACCC
ACTACAGGTACCTGAAGGAGTTCAGGACGGAGCAGTGCCCCCTGTTTTCACAGCACAAGTGCGCGCAGCA
CCGGCCGTTCACCTGCTTCCACTGGCACTTCCTCAACCAGCGGCGCCGCAGGCCCCTCCGCAGGCGCGAC
GGCACCTTCAACTACAGCCCCGACGTGTACTGCTCCAAGTACAACGAAGCCACCGGCGTGTGCCCCGACG
GCGACGAGTGTCCCTACCTGCACCGGACGACGGGGGACACAGAACGCAAGTACCACCTGCGTTACTACAA
AACAGGAACCTGCATCCACGAGACAGACGCACGTGGCCACTGCGTGAAGAATGGGCTGCACTGTGCCTTC
GCGCACGGCCCCCTGGACCTGCGGCCGCCCGTGTGTGACGTCAGGGAGCTGCAGGCCCAGGAAGCCTTGC
AGAACGGCCAGCTGGGCGGCGGGGAAGGGGTCCCGGATCTGCAGCCTGGGGTCTTGGCCAGCCAGGCCAT
GATTGAGAAGATCCTGAGCGAGGACCCCCGGTGGCAAGATGCCAACTTCGTGCTGGGCAGCTACAAGACG
GAGCAGTGCCCGAAGCCGCCACGCCTGTGCCGCCAGGGCTATGCGTGCCCACACTACCACAATAGCCGGG
ACAGGCGGCGCAACCCCCGGCGGTTCCAGTACAGGTCCACGCCCTGCCCCAGTGTGAAGCACGGGGATGA
GTGGGGGGAACCCTCACGCTGCGATGGCGGCGACGGCTGCCAGTATTGCCACTCCCGCACGGAGCAGCAG
TTCCATCCCGAGTCTACAAAATGCAACGACATGCGCCAAACCGGGTACTGCCCGCGCGGCCCCTTCTGTG
CCTTTGCACACGTTGAGAAGAGCCTGGGGATGGTGAATGAATGGGGCTGTCACGACCTCCACCTCACGAG
TCCTTCCTCCACGGGCAGCGGCCAGCCGGGAAATGCCAAGCGGAGAGACTCGCCGGCCGAGGGTGGCCCT
AGGGGCAGCGAGCAAGACAGCAAGCAGAACCACCTGGCCGTGTTTGCAGCGGTCCACCCGCCGGCCCCCA
GCGTGAGCTCCAGCGTGGCGTCCAGCCTGGCGTCCAGCGCCGGCTCCGGCAGCTCCTCCCCCACTGCGCT
GCCCGCGCCCCCCGCCCGTGCCCTCCCGCTCGGCCCCGCCAGCAGCACTGTGGAGGCCGTCCTCGGTTCT
GCGTTAGACCTGCATCTTAGCAATGTGAATATTGCATCCCTAGAGAAGGACCTGGAAGAGCAAGACGGCC
ACGACCTGGGAGCAGCAGGTCCCAGGTCGCTGGCCGGCTCTGCACCTGTCGCCATCCCCGGCTCCCTGCC
CAGAGCACCATCGCTACACTCGCCATCCTCTGCGTCCACCTCGCCGCTCGGTTCGCTGTCCCAGCCCCTC
CCAGGGCCGGTGGGCTCCTCAGCCATGACGCCTCCCCAGCAGCCGCCACCCCTGCGTTCAGAGCCGGGCA
CACTGGGCTCTGCAGCCTCATCCTACAGCCCCCTAGGTTTGAACGGTGTCCCCGGGAGCATCTGGGACTT
TGTTTCCGGCAGCTTCTCCCCCAGCCCCTCCCCCATCCTGAGTGCCGGCCCCCCATCCTCTTCGAGTGCA
AGTCCAAACGGAGCTGAGCTGGCCCGGGTCAGGCGGCAGCTGGACGAGGCCAAGAGGAAGATCCGGCAGT
GGGAGGAGTCCTGGCAGCAGGTGAAGCAGGTCTGCGATGCCTGGCAGCGAGAGGCGCAGGAGGCCAAGGA
GCGTGCCCGTGTGGCCGATAGCGACCGGCAGCTGGCGCTGCAGAAGAAGGAGGAGGTGGAGGCACAGGTG
ATCTTCCAGCTCCGCGCCAAGCAGTGTGTGGCCTGCCGGGAGCGGGCCCACGGTGCTGTCCTGCGGCCCT
GTCAGCACCACATCCTCTGTGAGCCGTGTGCGGCCACCGCACCTGAGTGCCCCTACTGCAAGGGCCAGCC
CCTGCAGTGG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC231370 representing NM_001193388
Red=Cloning site Green=Tags(s)

MPSVSKAAAAALSGSPPQTEKPTHYRYLKEFRTEQCPLFSQHKCAQHRPFTCFHWHFLNQRRRRPLRRRD
GTFNYSPDVYCSKYNEATGVCPDGDECPYLHRTTGDTERKYHLRYYKTGTCIHETDARGHCVKNGLHCAF
AHGPLDLRPPVCDVRELQAQEALQNGQLGGGEGVPDLQPGVLASQAMIEKILSEDPRWQDANFVLGSYKT
EQCPKPPRLCRQGYACPHYHNSRDRRRNPRRFQYRSTPCPSVKHGDEWGEPSRCDGGDGCQYCHSRTEQQ
FHPESTKCNDMRQTGYCPRGPFCAFAHVEKSLGMVNEWGCHDLHLTSPSSTGSGQPGNAKRRDSPAEGGP
RGSEQDSKQNHLAVFAAVHPPAPSVSSSVASSLASSAGSGSSSPTALPAPPARALPLGPASSTVEAVLGS
ALDLHLSNVNIASLEKDLEEQDGHDLGAAGPRSLAGSAPVAIPGSLPRAPSLHSPSSASTSPLGSLSQPL
PGPVGSSAMTPPQQPPPLRSEPGTLGSAASSYSPLGLNGVPGSIWDFVSGSFSPSPSPILSAGPPSSSSA
SPNGAELARVRRQLDEAKRKIRQWEESWQQVKQVCDAWQREAQEAKERARVADSDRQLALQKKEEVEAQV
IFQLRAKQCVACRERAHGAVLRPCQHHILCEPCAATAPECPYCKGQPLQW

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001193388
ORF Size 2040 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001193388.1, NP_001180317.1
RefSeq ORF 2052 bp
Locus ID 64718
UniProt ID Q9H9P5, A0A0A0MRZ1
Cytogenetics 16p13.3
Protein Families Druggable Genome
MW 74.7 kDa
Summary This gene encodes a RING finger protein that may function in Rac signaling. It can bind to Brg/Brm-associated factor 60b and can promote its ubiquitination. Alternative splicing of this gene results in multiple transcript variants. provided by RefSeq, Jun 2013
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"NM_001193388" in other vectors (3)
SKU Description Size Price
RC231370L3 Lenti-ORF clone of UNKL (Myc-DDK-tagged)-Human unkempt homolog (Drosophila)-like (UNKL), transcript variant 1 10 ug
$923.00
RC231370L4 Lenti-ORF clone of UNKL (mGFP-tagged)-Human unkempt homolog (Drosophila)-like (UNKL), transcript variant 1 10 ug
$923.00
RG231370 UNKL (tGFP-tagged) - Human unkempt homolog (Drosophila)-like (UNKL), transcript variant 1 10 ug
$823.00

Plasmid Map for RC231370

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC231370
Circular map for RC231370

file-alt Citations

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