ODF2 (NM_153436) Human Tagged ORF Clone

SKU RC231359

ODF2 (Myc-DDK-tagged)-Human outer dense fiber of sperm tails 2 (ODF2), transcript variant 4

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: ODF2
• Synonyms: CT134; ODF2/1; ODF2/2; ODF84
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC231359 representing NM_153436; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCGGACCAGCAAGGACCTCATCAGAATAGGTTTCCATCGTGTGGGAAGAACGGAGTAACGAGTCTCA; CGCAGAAAAAGGTCTTGAGAGCACCTTGTGGCGCACCCAGTGTAACTGTGACGAAATCTCACAAGCGAGG; AATGAAAGGGGACACTGTGAATGTGCGGCGGAGTGTCCGGGTGAAAACCAAGGTACCTTGGATGCCCCCT; GGAAAATCATCTGCCCGGCCTGTGGGATGCAAGTGGGAGAATCCACCTCATTGCCTGGAGATCACGCCAC; CATCTTCAGAAAAGCTGGTCTCAGTGATGCGGTTAAGTGACCTCTCTACAGAAGATGATGACTCAGGTCA; CTGTAAAATGAACCGTTATGATAAGAAGATTGATAGTCTAATGAATGCGGTTGGTTGTCTGAAGTCTGAG; GTCAAGATGCAAAAAGGTGAGCGCCAGATGGCCAAAAGGTTCCTGGAGGAACGGAAGGAAGAGCTGGAGG; AGGTGGCCCACGAACTGGCTGAGACTGAGCACGAGAACACGGTGTTGAGGCACAACATCGAGCGCATGAA; GGAGGAGAAGGACTTCACCATACTTCAGAAGAAACACCTACAACAGGAGAAGGAGTGCCTCATGTCCAAG; CTGGTGGAGGCGGAAATGGATGGGGCTGCGGCTGCCAAGCAGGTCATGGCCTTGAAGGATACCATCGGGA; AGCTGAAAACGGAGAAACAAATGACCTGCACGGACATCAACACCCTGACAAGGCAGAAGGAACTTCTCCT; GCAGAAGCTGAGCACATTTGAGGAGACCAACCGCACCCTCCGAGACCTCCTGAGGGAACAGCACTGCAAA; GAGGATTCTGAAAGACTAATGGAGCAACAAGGAGCACTGCTGAAACGGCTGGCGGAGGCCGACTCAGAGA; AAGCGCGCCTGCTGTTACTGCTGCAAGACAAGGACAAGGAGGTGGAAGAGCTCCTTCAGGAAATACAATG; TGAGAAGGCTCAAGCAAAGACAGCCTCTGAGCTTTCTAAATCCATGGAGTCCATGCGTGGGCATTTGCAG; GCACAGCTTCGGTCCAAAGAGGCTGAGAACAGTCGCCTGTGCATGCAGATTAAGAATCTGGAGCGCAGCG; GGAATCAGCATAAGGCAGAAGTGGAGGCCATCATGGAGCAGCTGAAGGAGTTGAAGCAGAAGGGAGACCG; AGACAAAGAGAGCTTGAAGAAGGCCATCCGAGCCCAGAAGGAGCGAGCCGAGAAGAGCGAGGAGTATGCT; GAGCAGCTACACGTGCAACTCGCTGACAAGGATCTTTATGTCGCTGAAGCTTTATCCACTCTGGAATCCT; GGAGGAGCCGCTACAACCAAGTTGTAAAAGAAAAGGGAGACCTTGAGCTGGAAATTATTGTCCTGAATGA; CCGGGTAACAGATCTTGTAAACCAACAACAAACCCTGGAGGAGAAGATGCGGGAAGACCGGGATAGCCTG; GTGGAGAGACTACACCGTCAGACTGCTGAGTATTCCGCATTCAAGCTGGAGAATGAGAGGCTGAAGGCCA; GCTTTGCTCCAATGGAGGACAAACTCAACCAGGCACACCTCGAGGTCCAGCAGCTGAAGGCCTCAGTGAA; GAACTATGAGGGGATGATTGACAACTATAAGAGTCAGGTGATGAAGACCAGATTGGAGGCTGATGAAGTA; GCTGCCCAGCTAGAACGCTGTGACAAAGAGAACAAGATCCTTAAAGATGAGATGAACAAAGAGATTGAGG; CGGCACGAAGGCAGTTCCAGTCTCAGCTGGCTGACCTGCAGCAGCTCCCTGACATCCTGAAGATCACGGA; GGCGAAGCTGGCTGAGTGCCAAGACCAACTGCAGGGCTATGAGCGGAAGAACATCGACCTCACAGCCATC; ATATCAGACCTGCGCAGCCGGGTAAGGGACTGGCAGAAAGGGTCCCACGAACTGACCCGAGCAGGGGCCC; GCATACCAAGA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC231359 representing NM_153436; Red=Cloning site Green=Tags(s); ; MADQQGPHQNRFPSCGKNGVTSLTQKKVLRAPCGAPSVTVTKSHKRGMKGDTVNVRRSVRVKTKVPWMPP; GKSSARPVGCKWENPPHCLEITPPSSEKLVSVMRLSDLSTEDDDSGHCKMNRYDKKIDSLMNAVGCLKSE; VKMQKGERQMAKRFLEERKEELEEVAHELAETEHENTVLRHNIERMKEEKDFTILQKKHLQQEKECLMSK; LVEAEMDGAAAAKQVMALKDTIGKLKTEKQMTCTDINTLTRQKELLLQKLSTFEETNRTLRDLLREQHCK; EDSERLMEQQGALLKRLAEADSEKARLLLLLQDKDKEVEELLQEIQCEKAQAKTASELSKSMESMRGHLQ; AQLRSKEAENSRLCMQIKNLERSGNQHKAEVEAIMEQLKELKQKGDRDKESLKKAIRAQKERAEKSEEYA; EQLHVQLADKDLYVAEALSTLESWRSRYNQVVKEKGDLELEIIVLNDRVTDLVNQQQTLEEKMREDRDSL; VERLHRQTAEYSAFKLENERLKASFAPMEDKLNQAHLEVQQLKASVKNYEGMIDNYKSQVMKTRLEADEV; AAQLERCDKENKILKDEMNKEIEAARRQFQSQLADLQQLPDILKITEAKLAECQDQLQGYERKNIDLTAI; ISDLRSRVRDWQKGSHELTRAGARIPR; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153436
• ORF Size: 1971 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_153436.1, NP_702914.1
• RefSeq ORF: 1974 bp
• Locus ID: 4957
• UniProt ID: Q5BJF6
• Cytogenetics: 9q34.11
• MW: 76.1 kDa
• Summary: The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Alternative splicing results in multiple transcript variants. The longer transcripts, also known as 'Cenexins', encode proteins with a C-terminal extension that are differentially targeted to somatic centrioles and thought to be crucial for the formation of microtubule organizing centers. [provided by RefSeq, Oct 2010]