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ODF2 (NM_153436) Human Tagged ORF Clone

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SKU
RC231359
ODF2 (Myc-DDK-tagged)-Human outer dense fiber of sperm tails 2 (ODF2), transcript variant 4
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $612.00
In Stock*
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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ODF2
Synonyms CT134; ODF2/1; ODF2/2; ODF84
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC231359 representing NM_153436
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACCAGCAAGGACCTCATCAGAATAGGTTTCCATCGTGTGGGAAGAACGGAGTAACGAGTCTCA
CGCAGAAAAAGGTCTTGAGAGCACCTTGTGGCGCACCCAGTGTAACTGTGACGAAATCTCACAAGCGAGG
AATGAAAGGGGACACTGTGAATGTGCGGCGGAGTGTCCGGGTGAAAACCAAGGTACCTTGGATGCCCCCT
GGAAAATCATCTGCCCGGCCTGTGGGATGCAAGTGGGAGAATCCACCTCATTGCCTGGAGATCACGCCAC
CATCTTCAGAAAAGCTGGTCTCAGTGATGCGGTTAAGTGACCTCTCTACAGAAGATGATGACTCAGGTCA
CTGTAAAATGAACCGTTATGATAAGAAGATTGATAGTCTAATGAATGCGGTTGGTTGTCTGAAGTCTGAG
GTCAAGATGCAAAAAGGTGAGCGCCAGATGGCCAAAAGGTTCCTGGAGGAACGGAAGGAAGAGCTGGAGG
AGGTGGCCCACGAACTGGCTGAGACTGAGCACGAGAACACGGTGTTGAGGCACAACATCGAGCGCATGAA
GGAGGAGAAGGACTTCACCATACTTCAGAAGAAACACCTACAACAGGAGAAGGAGTGCCTCATGTCCAAG
CTGGTGGAGGCGGAAATGGATGGGGCTGCGGCTGCCAAGCAGGTCATGGCCTTGAAGGATACCATCGGGA
AGCTGAAAACGGAGAAACAAATGACCTGCACGGACATCAACACCCTGACAAGGCAGAAGGAACTTCTCCT
GCAGAAGCTGAGCACATTTGAGGAGACCAACCGCACCCTCCGAGACCTCCTGAGGGAACAGCACTGCAAA
GAGGATTCTGAAAGACTAATGGAGCAACAAGGAGCACTGCTGAAACGGCTGGCGGAGGCCGACTCAGAGA
AAGCGCGCCTGCTGTTACTGCTGCAAGACAAGGACAAGGAGGTGGAAGAGCTCCTTCAGGAAATACAATG
TGAGAAGGCTCAAGCAAAGACAGCCTCTGAGCTTTCTAAATCCATGGAGTCCATGCGTGGGCATTTGCAG
GCACAGCTTCGGTCCAAAGAGGCTGAGAACAGTCGCCTGTGCATGCAGATTAAGAATCTGGAGCGCAGCG
GGAATCAGCATAAGGCAGAAGTGGAGGCCATCATGGAGCAGCTGAAGGAGTTGAAGCAGAAGGGAGACCG
AGACAAAGAGAGCTTGAAGAAGGCCATCCGAGCCCAGAAGGAGCGAGCCGAGAAGAGCGAGGAGTATGCT
GAGCAGCTACACGTGCAACTCGCTGACAAGGATCTTTATGTCGCTGAAGCTTTATCCACTCTGGAATCCT
GGAGGAGCCGCTACAACCAAGTTGTAAAAGAAAAGGGAGACCTTGAGCTGGAAATTATTGTCCTGAATGA
CCGGGTAACAGATCTTGTAAACCAACAACAAACCCTGGAGGAGAAGATGCGGGAAGACCGGGATAGCCTG
GTGGAGAGACTACACCGTCAGACTGCTGAGTATTCCGCATTCAAGCTGGAGAATGAGAGGCTGAAGGCCA
GCTTTGCTCCAATGGAGGACAAACTCAACCAGGCACACCTCGAGGTCCAGCAGCTGAAGGCCTCAGTGAA
GAACTATGAGGGGATGATTGACAACTATAAGAGTCAGGTGATGAAGACCAGATTGGAGGCTGATGAAGTA
GCTGCCCAGCTAGAACGCTGTGACAAAGAGAACAAGATCCTTAAAGATGAGATGAACAAAGAGATTGAGG
CGGCACGAAGGCAGTTCCAGTCTCAGCTGGCTGACCTGCAGCAGCTCCCTGACATCCTGAAGATCACGGA
GGCGAAGCTGGCTGAGTGCCAAGACCAACTGCAGGGCTATGAGCGGAAGAACATCGACCTCACAGCCATC
ATATCAGACCTGCGCAGCCGGGTAAGGGACTGGCAGAAAGGGTCCCACGAACTGACCCGAGCAGGGGCCC
GCATACCAAGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC231359 representing NM_153436
Red=Cloning site Green=Tags(s)

MADQQGPHQNRFPSCGKNGVTSLTQKKVLRAPCGAPSVTVTKSHKRGMKGDTVNVRRSVRVKTKVPWMPP
GKSSARPVGCKWENPPHCLEITPPSSEKLVSVMRLSDLSTEDDDSGHCKMNRYDKKIDSLMNAVGCLKSE
VKMQKGERQMAKRFLEERKEELEEVAHELAETEHENTVLRHNIERMKEEKDFTILQKKHLQQEKECLMSK
LVEAEMDGAAAAKQVMALKDTIGKLKTEKQMTCTDINTLTRQKELLLQKLSTFEETNRTLRDLLREQHCK
EDSERLMEQQGALLKRLAEADSEKARLLLLLQDKDKEVEELLQEIQCEKAQAKTASELSKSMESMRGHLQ
AQLRSKEAENSRLCMQIKNLERSGNQHKAEVEAIMEQLKELKQKGDRDKESLKKAIRAQKERAEKSEEYA
EQLHVQLADKDLYVAEALSTLESWRSRYNQVVKEKGDLELEIIVLNDRVTDLVNQQQTLEEKMREDRDSL
VERLHRQTAEYSAFKLENERLKASFAPMEDKLNQAHLEVQQLKASVKNYEGMIDNYKSQVMKTRLEADEV
AAQLERCDKENKILKDEMNKEIEAARRQFQSQLADLQQLPDILKITEAKLAECQDQLQGYERKNIDLTAI
ISDLRSRVRDWQKGSHELTRAGARIPR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_153436
ORF Size 1971 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_153436.1, NP_702914.1
RefSeq ORF 1974 bp
Locus ID 4957
UniProt ID Q5BJF6
Cytogenetics 9q34.11
MW 76.1 kDa
Summary The outer dense fibers are cytoskeletal structures that surround the axoneme in the middle piece and principal piece of the sperm tail. The fibers function in maintaining the elastic structure and recoil of the sperm tail as well as in protecting the tail from shear forces during epididymal transport and ejaculation. Defects in the outer dense fibers lead to abnormal sperm morphology and infertility. This gene encodes one of the major outer dense fiber proteins. Alternative splicing results in multiple transcript variants. The longer transcripts, also known as 'Cenexins', encode proteins with a C-terminal extension that are differentially targeted to somatic centrioles and thought to be crucial for the formation of microtubule organizing centers. provided by RefSeq, Oct 2010
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"NM_153436" in other vectors (3)
SKU Description Size Price
RC231359L3 Lenti-ORF clone of ODF2 (Myc-DDK-tagged)-Human outer dense fiber of sperm tails 2 (ODF2), transcript variant 4 10 ug
$912.00
RC231359L4 Lenti-ORF clone of ODF2 (mGFP-tagged)-Human outer dense fiber of sperm tails 2 (ODF2), transcript variant 4 10 ug
$912.00
RG231359 ODF2 (tGFP-tagged) - Human outer dense fiber of sperm tails 2 (ODF2), transcript variant 4 10 ug
$812.00

Plasmid Map for RC231359

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC231359
Circular map for RC231359

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