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FAPP2 (PLEKHA8) (NM_001197027) Human Tagged ORF Clone

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SKU
RC231247
PLEKHA8 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8 (PLEKHA8), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $457.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol FAPP2
Synonyms FAPP2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC231247 representing NM_001197027
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGGGGGTGCTGTACAAGTGGACCAACTATCTGAGCGGTTGGCAGCCTCGATGGTTCCTTCTCTGTG
GGGGAATATTGTCCTATTATGATTCTCCTGAAGATGCCTGGAAAGGTTGCAAAGGGAGCATACAAATGGC
AGTCTGTGAAATTCAAGTTCATTCTGTAGATAATACACGCATGGACCTGATAATCCCTGGGGAACAGTAT
TTCTACCTGAAGGCCAGAAGTGTGGCTGAAAGACAGCGGTGGCTGGTGGCCCTGGGATCAGCCAAGGCTT
GCCTGACTGACAGTAGGACCCAGAAGGAGAAAGAGTTTGCTGAAAACACTGAAAACTTGAAAACCAAAAT
GTCAGAACTAAGACTCTACTGTGACCTCCTTGTTCAGCAAGTAGATAAAACAAAAGAAGTGACCACAACT
GGTGTGTCCAATTCTGAGGAGGGAATTGATGTGGGAACTTTGCTGAAATCAACCTGTAATACTTTTCTGA
AGACCTTGGAAGAATGCATGCAGATCGCAAATGCAGCCTTCACCTCTGAGCTGCTCTACCGCACTCCACC
AGGATCACCTCAGCTGGCCATGCTCAAGTCCAGCAAGATGAAACATCCTATTATACCAATTCATAATTCA
TTGGAAAGGCAAATGGAGTTGAGCACTTGTGAAAATGGATCTTTAAATATGGAAATAAATGGTGAGGAAG
AAATCCTAATGAAAAATAAGAATTCCTTATATTTGAAATCTGCAGAGATAGACTGCAGCATATCAAGTGA
GGAAAATACAGATGATAATATAACAGTCCAAGGTGAAATAAGGAAGGAAGATGGAATGGAAAACCTGAAA
AATCATGACAATAACTTGACTCAGTCTGGATCAGACTCAAGTTGCTCTCCGGAATGCCTCTGGGAGGAAG
GCAAAGAAGTTATCCCAACTTTCTTTAGTACCATGAACACAAGCTTTAGTGACATTGAACTTCTGGAAGA
CAGTGGCATTCCCACAGAAGCATTCTTGGCATCATGTTATGCTGTGGTTCCAGTATTAGACAAACTTGGC
CCTACAGTGTTTGCTCCTGTTAAGATGGATCTTGTTGGAAATATTAAGAAAGTAAATCAGAAGTATATAA
CCAACAAAGAAGAGTTTACCACTCTCCAGAAGATAGTGCTGCACGAAGTGGAGGCGGATGTAGCCCAGGT
TAGGAACTCAGCGACTGAAGCCCTCTTGTGGCTGAAGAGAGGTCTCAAATTTTTGAAGGGATTTTTGACA
GAAGTGAAAAATGGGGAGAAGGATATCCAGACAGCCCTAAATAATGCATATGGTAAAACATTGCGGCAAC
ACCATGGCTGGGTAGTTCGAGGGGTTTTTGCGGGACACACAAATGTC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC231247 representing NM_001197027
Red=Cloning site Green=Tags(s)

MEGVLYKWTNYLSGWQPRWFLLCGGILSYYDSPEDAWKGCKGSIQMAVCEIQVHSVDNTRMDLIIPGEQY
FYLKARSVAERQRWLVALGSAKACLTDSRTQKEKEFAENTENLKTKMSELRLYCDLLVQQVDKTKEVTTT
GVSNSEEGIDVGTLLKSTCNTFLKTLEECMQIANAAFTSELLYRTPPGSPQLAMLKSSKMKHPIIPIHNS
LERQMELSTCENGSLNMEINGEEEILMKNKNSLYLKSAEIDCSISSEENTDDNITVQGEIRKEDGMENLK
NHDNNLTQSGSDSSCSPECLWEEGKEVIPTFFSTMNTSFSDIELLEDSGIPTEAFLASCYAVVPVLDKLG
PTVFAPVKMDLVGNIKKVNQKYITNKEEFTTLQKIVLHEVEADVAQVRNSATEALLWLKRGLKFLKGFLT
EVKNGEKDIQTALNNAYGKTLRQHHGWVVRGVFAGHTNV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001197027
ORF Size 1377 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001197027.1, NP_001183956.1
RefSeq ORF 1380 bp
Locus ID 84725
UniProt ID Q96JA3, A0A087X1S6
Cytogenetics 7p14.3
MW 52 kDa
Summary Cargo transport protein that is required for apical transport from the Golgi complex. Transports AQP2 from the trans-Golgi network (TGN) to sites of AQP2 phosphorylation. Mediates the non-vesicular transport of glucosylceramide (GlcCer) from the trans-Golgi network (TGN) to the plasma membrane and plays a pivotal role in the synthesis of complex glycosphingolipids. Binding of both phosphatidylinositol 4-phosphate (PIP) and ARF1 are essential for the GlcCer transfer ability. Also required for primary cilium formation, possibly by being involved in the transport of raft lipids to the apical membrane, and for membrane tubulation.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001197027" in other vectors (3)
SKU Description Size Price
RC231247L3 Lenti-ORF clone of PLEKHA8 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8 (PLEKHA8), transcript variant 2 10 ug
$757.00
RC231247L4 Lenti-ORF clone of PLEKHA8 (mGFP-tagged)-Human pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8 (PLEKHA8), transcript variant 2 10 ug
$757.00
RG231247 PLEKHA8 (tGFP-tagged) - Human pleckstrin homology domain containing, family A (phosphoinositide binding specific) member 8 (PLEKHA8), transcript variant 2 10 ug
$657.00

Plasmid Map for RC231247

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC231247
Circular map for RC231247

file-alt Citations

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