ENO3 (NM_001193503) Human Tagged ORF Clone

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SKU
RC231202
ENO3 (Myc-DDK-tagged)-Human enolase 3 (beta, muscle) (ENO3), transcript variant 3
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol ENO3
Synonyms GSD13; MSE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC231202 representing NM_001193503
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCATGCAGAAAATCTTTGCCCGGGAAATCTTGGACTCCAGGGGCAACCCCACGGTGGAGGTGGACC
TGCACACGGCCAAGGGCCGATTCCGAGCAGCTGTGCCCAGTGGGGCTTCCACGGGTATCTATGAGGCTCT
GGAACTAAGAGACGGAGACAAAGGCCGCTACCTGGGGAAAGCCAAGTTTGGGGCCAATGCCATCCTGGGC
GTGTCCTTGGCCGTGTGTAAGGCGGGAGCAGCTGAGAAGGGGGTCCCCCTGTACCGCCACATCGCAGATC
TCGCTGGGAACCCTGACCTCATACTCCCAGTGCCAGCCTTCAATGTGATCAACGGGGGCTCCCATGCTGG
AAACAAGCTGGCCATGCAGGAGTTCATGATTCTGCCTGTGGGAGCCAGCTCCTTCAAGGAAGCCATGCGC
ATTGGCGCCGAGGTCTACCACCACCTCAAGGGGGTCATCAAGGCCAAGTATGGGAAGGATGCCACCAATG
TGGGTGATGAAGGTGGCTTCGCACCCAACATCCTGGAGAACAATGAGGCCCTGGAGCTGCTGAAGACGGC
CATCCAGGCGGCTGGTTACCCAGACAAGGTGGTGATCGGCATGGATGTGGCAGCATCTGAGTTCTATCGC
AATGGGAAGTACGATCTTGACTTCAAGTCGCCTGATGATCCCGCACGGCACATCACTGGGGAGAAGCTCG
GAGAGCTGTATAAGAGCTTTATCAAGAACTATCCTGTGGTCTCCATCGAAGACCCCTTTGACCAGGATGA
CTGGGCCACTTGGACCTCCTTCCTCTCGGGGGTGAACATCCAGATTGTGGGGGATGACTTGACAGTCACC
AACCCCAAGAGGATTGCCCAGGCCGTTGAGAAGAAGGCCTGCAACTGTCTGCTGCTGAAGGTCAACCAGA
TCGGCTCGGTGACCGAATCGATCCAGGCGTGCAAACTGGCTCAGTCTAATGGCTGGGGGGTGATGGTGAG
CCACCGCTCTGGGGAGACTGAGGACACATTCATTGCTGACCTTGTGGTGGGGCTCTGCACAGGACAGATC
AAGACTGGCGCCCCCTGCCGCTCGGAGCGTCTGGCCAAATACAACCAACTCATGAGGATCGAGGAGGCTC
TTGGGGACAAGGCAATCTTTGCTGGACGCAAGTTCCGTAACCCGAAGGCCAAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC231202 representing NM_001193503
Red=Cloning site Green=Tags(s)

MAMQKIFAREILDSRGNPTVEVDLHTAKGRFRAAVPSGASTGIYEALELRDGDKGRYLGKAKFGANAILG
VSLAVCKAGAAEKGVPLYRHIADLAGNPDLILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFKEAMR
IGAEVYHHLKGVIKAKYGKDATNVGDEGGFAPNILENNEALELLKTAIQAAGYPDKVVIGMDVAASEFYR
NGKYDLDFKSPDDPARHITGEKLGELYKSFIKNYPVVSIEDPFDQDDWATWTSFLSGVNIQIVGDDLTVT
NPKRIAQAVEKKACNCLLLKVNQIGSVTESIQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQI
KTGAPCRSERLAKYNQLMRIEEALGDKAIFAGRKFRNPKAK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001193503
ORF Size 1173 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001193503.2
RefSeq ORF 1176 bp
Locus ID 2027
UniProt ID P13929
Cytogenetics 17p13.2
Protein Pathways Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation
MW 42.7 kDa
Summary This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.provided by RefSeq, Jul 2010
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Documents
Resources
"NM_001193503" in other vectors (3)
SKU Description Size Price
RC231202L3 Lenti ORF clone of Human enolase 3 (beta, muscle) (ENO3), transcript variant 3, Myc-DDK-tagged 10 ug
$757.00
RC231202L4 Lenti ORF clone of Human enolase 3 (beta, muscle) (ENO3), transcript variant 3, mGFP tagged 10 ug
$757.00
RG231202 ENO3 (tGFP-tagged) - Human enolase 3 (beta, muscle) (ENO3), transcript variant 3 10 ug
$657.00

Plasmid Map for RC231202

Download sequence for

Cloning scheme for RC231202

Circular map for RC231202

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