DDIT3 (NM_001195053) Human Tagged ORF Clone
SKU
RC231040
DDIT3 (Myc-DDK-tagged)-Human DNA-damage-inducible transcript 3 (DDIT3), transcript variant 1
$450.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | DDIT3 |
| Synonyms | AltDDIT3; C/EBPzeta; CEBPZ; CHOP; CHOP-10; CHOP10; GADD153 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001195053 |
| ORF Size | 576 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001195053.1, NP_001181982.1 |
| RefSeq ORF | 579 bp |
| Locus ID | 1649 |
| UniProt ID | P35638 |
| Cytogenetics | 12q13.3 |
| Protein Families | Druggable Genome, Transcription Factors |
| Protein Pathways | MAPK signaling pathway |
| MW | 22.1 kDa |
| Summary | This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. provided by RefSeq, Aug 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001195053" in other vectors (5)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC231040L1 | Lenti ORF clone of Human DNA-damage-inducible transcript 3 (DDIT3), transcript variant 1, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC231040L2 | Lenti ORF clone of Human DNA-damage-inducible transcript 3 (DDIT3), transcript variant 1, mGFP tagged | 10 ug |
$750.00
|
|
| RC231040L3 | Lenti ORF clone of Human DNA-damage-inducible transcript 3 (DDIT3), transcript variant 1, Myc-DDK-tagged | 10 ug |
$750.00
|
|
| RC231040L4 | Lenti ORF clone of Human DNA-damage-inducible transcript 3 (DDIT3), transcript variant 1, mGFP tagged | 10 ug |
$750.00
|
|
| RG231040 | DDIT3 (tGFP-tagged) - Human DNA-damage-inducible transcript 3 (DDIT3), transcript variant 1 | 10 ug |
$650.00
|
|
Citations
miR-1271 inhibits ERα expression and confers letrozole resistance in breast cancer
Yu, Yu, Sun et al
Oncotarget (2017) 8 (63), 107134-107148 DOI: 10.18632/oncotarget.22359
Yu, Yu, Sun et al
Oncotarget (2017) 8 (63), 107134-107148 DOI: 10.18632/oncotarget.22359
Snippet: mu-ESR1-promoter). For the subsequent ChIP assay, pCI-ESR1-promoter or pCI-mu-ESR1-promoter were co-transfected with pCMV-DDK-tagged-DDIT3 (OriGene Technologies, Beijing, China) into HeLa cells. This cell type
Differential expression of endoplasmic reticulum stress-response proteins in different renal tubule subtypes of OVE26 diabetic mice
Barati, Powell, Kechavarzi et al
Cell Stress Chaperones (2015)
Barati, Powell, Kechavarzi et al
Cell Stress Chaperones (2015)
Cited by 15
AI Summary: OriGene's CHOP DNA plasmid was used to overexpress CHOP in cultured proximal tubule cells, enabling the study of CHOP's role in fibronectin expression and ER stress response in diabetic mouse kidney tubules.
Snippet: For overexpression of CHOP, cells were grown to 85 % confluence and subject to transfection with 2 μg CHOP DNA (Origene, Rockville, MD) or vector control for 24 h, using Lipofectamine 2000. ER stress markers in different renal tubules of type 1 diabetic mice Page ... Cite SaveSaving
Showing 1-2 of 2 papers.
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