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BRD7 (NM_001173984) Human Tagged ORF Clone

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SKU
RC230395
BRD7 (Myc-DDK-tagged)-Human bromodomain containing 7 (BRD7), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $607.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol BRD7
Synonyms BP75; CELTIX1; NAG4
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC230395 representing NM_001173984
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCAAGAAGCACAAGAAGCACAAGTCGGACAAACACCTCTACGAGGAGTATGTAGAGAAGCCCTTGA
AGCTGGTCCTCAAAGTAGGAGGGAACGAAGTCACCGAACTCTCCACGGGCAGCTCGGGGCACGACTCCAG
CCTCTTCGAAGACAAAAACGATCATGACAAACACAAGGACAGAAAGCGGAAAAAGAGAAAGAAAGGAGAG
AAGCAGATTCCAGGGGAAGAAAAGGGGAGAAAACGGAGAAGAGTTAAGGAGGATAAAAAGAAGCGAGATC
GAGACCGGGTGGAGAATGAGGCAGAAAAAGATCTCCAGTGTCACGCCCCTGTGAGATTAGACTTGCCTCC
TGAGAAGCCTCTCACAAGCTCTTTAGCCAAACAAGAAGAAGTAGAACAGACACCCCTTCAAGAAGCTTTG
AATCAACTGATGAGACAATTGCAGAGAAAAGATCCAAGTGCTTTCTTTTCATTTCCTGTGACTGATTTTA
TTGCTCCTGGCTACTCCATGATCATTAAACACCCAATGGATTTTAGTACCATGAAAGAAAAGATCAAGAA
CAATGACTATCAGTCCATAGAAGAACTAAAGGATAACTTCAAACTAATGTGTACTAATGCCATGATTTAC
AATAAACCAGAGACCATTTATTATAAAGCTGCAAAGAAGCTGTTGCACTCAGGAATGAAAATTCTTAGCC
AGGAAAGAATTCAGAGCCTGAAGCAGAGCATAGACTTCATGGCTGACTTGCAGAAAACTCGAAAGCAGAA
AGATGGAACAGACACCTCACAGAGTGGGGAGGACGGAGGCTGCTGGCAGAGAGAGAGAGAGGACTCTGGA
GATGCCGAAGCACACGCCTTCAAGAGTCCCAGCAAAGAAAATAAAAAGAAAGACAAAGATATGCTTGAAG
ATAAGTTTAAAAGCAATAATTTAGAGAGAGAGCAGGAGCAGCTTGACCGCATCGTGAAGGAATCTGGAGG
AAAGCTGACCAGGCGGCTTGTGAACAGTCAGTGCGAATTTGAAAGAAGAAAACCAGATGGAACAACGACG
TTGGGACTTCTCCATCCTGTGGATCCCATTGTAGGAGAGCCAGGCTACTGCCCTGTGAGACTGGGAATGA
CAACTGGAAGACTTCAGTCTGGAGTGAATACTTTGCAGGGGTTCAAAGAGGATAAAAGGAACAAAGTCAC
TCCAGTGTTATATTTGAATTATGGGCCCTACAGTTCTTATGCACCGCATTATGACTCCACATTTGCAAAT
ATCAGCAAGGATGATTCTGATTTAATCTATTCAACCTATGGGGAAGACTCTGATCTTCCAAGTGATTTCA
GCATCCATGAGTTTTTGGCCACGTGCCAAGATTATCCGTATGTCATGGCAGATAGTTTACTGGATGTTTT
AACAAAAGGAGGGCATTCCAGGACCCTACAAGAGATGGAGATGTCATTGCCTGAAGATGAAGGCCATACT
AGGACACTTGACACAGCAAAAGAAATGGAGCAGATTACAGAAGTAGAGCCACCAGGGCGTTTGGACTCCA
GTACTCAAGACAGGCTCATAGCGCTGAAAGCAGTAACAAATTTTGGCGTTCCAGTTGAAGTTTTTGACTC
TGAAGAAGCTGAAATATTCCAGAAGAAACTTGATGAGACCACCAGATTGCTCAGGGAACTCCAGGAAGCC
CAGAATGAACGTTTGAGCACCAGACCCCCTCCGAACATGATCTGTCTCTTGGGTCCCTCATACAGAGAAA
TGCATCTTGCTGAACAAGTGACCAATAATCTTAAAGAACTTGCACAGCAAGTAACTCCAGGTGATATCGT
AAGCACGTATGGAGTTCGAAAAGCAATGGGGATTTCCATTCCTTCCCCCGTCATGGAAAACAACTTTGTG
GATTTGACAGAAGACACTGAAGAACCTAAAAAGACGGATGTTGCTGAGTGTGGACCTGGTGGAAGT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC230395 representing NM_001173984
Red=Cloning site Green=Tags(s)

MGKKHKKHKSDKHLYEEYVEKPLKLVLKVGGNEVTELSTGSSGHDSSLFEDKNDHDKHKDRKRKKRKKGE
KQIPGEEKGRKRRRVKEDKKKRDRDRVENEAEKDLQCHAPVRLDLPPEKPLTSSLAKQEEVEQTPLQEAL
NQLMRQLQRKDPSAFFSFPVTDFIAPGYSMIIKHPMDFSTMKEKIKNNDYQSIEELKDNFKLMCTNAMIY
NKPETIYYKAAKKLLHSGMKILSQERIQSLKQSIDFMADLQKTRKQKDGTDTSQSGEDGGCWQREREDSG
DAEAHAFKSPSKENKKKDKDMLEDKFKSNNLEREQEQLDRIVKESGGKLTRRLVNSQCEFERRKPDGTTT
LGLLHPVDPIVGEPGYCPVRLGMTTGRLQSGVNTLQGFKEDKRNKVTPVLYLNYGPYSSYAPHYDSTFAN
ISKDDSDLIYSTYGEDSDLPSDFSIHEFLATCQDYPYVMADSLLDVLTKGGHSRTLQEMEMSLPEDEGHT
RTLDTAKEMEQITEVEPPGRLDSSTQDRLIALKAVTNFGVPVEVFDSEEAEIFQKKLDETTRLLRELQEA
QNERLSTRPPPNMICLLGPSYREMHLAEQVTNNLKELAQQVTPGDIVSTYGVRKAMGISIPSPVMENNFV
DLTEDTEEPKKTDVAECGPGGS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001173984
ORF Size 1956 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001173984.3
RefSeq ORF 1959 bp
Locus ID 29117
UniProt ID Q9NPI1
Cytogenetics 16q12.1
MW 74.7 kDa
Summary This gene encodes a protein which is a member of the bromodomain-containing protein family. The product of this gene has been identified as a component of one form of the SWI/SNF chromatin remodeling complex, and as a protein which interacts with p53 and is required for p53-dependent oncogene-induced senescence which prevents tumor growth. Pseudogenes have been described on chromosomes 2, 3, 6, 13 and 14. Alternative splicing results in multiple transcript variants. provided by RefSeq, Jul 2010
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"NM_001173984" in other vectors (6)
SKU Description Size Price
RC230395L1 Lenti-ORF clone of BRD7 (Myc-DDK-tagged)-Human bromodomain containing 7 (BRD7), transcript variant 1 10 ug
$907.00
RC230395L2 Lenti-ORF clone of BRD7 (mGFP-tagged)-Human bromodomain containing 7 (BRD7), transcript variant 1 10 ug
$907.00
RC230395L3 Lenti-ORF clone of BRD7 (Myc-DDK-tagged)-Human bromodomain containing 7 (BRD7), transcript variant 1 10 ug
$907.00
RC230395L4 Lenti-ORF clone of BRD7 (mGFP-tagged)-Human bromodomain containing 7 (BRD7), transcript variant 1 10 ug
$907.00
RG230395 BRD7 (tGFP-tagged) - Human bromodomain containing 7 (BRD7), transcript variant 1 10 ug
$807.00
SC329033 BRD7 (untagged)-Human bromodomain containing 7 (BRD7) transcript variant 1 10 ug
$608.00

Plasmid Map for RC230395

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC230395
Circular map for RC230395

file-alt Citations

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