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TXNRD3 (NM_001173513) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC230340
TXNRD3 (Myc-DDK-tagged)-Human thioredoxin reductase 3 (TXNRD3), transcript variant 2, (Note, selenocysteine protein, internal stop codon, see reference data summary)
ORF Plasmid:
AAV Particle:
DDK
$289.00 $544.00
In Stock*
Bulk Requests & Clone Modifications

Frequently Bought Together (6)

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Other products for "TXNRD3 or NM_001173513"

Specifications
Specifications
Product Data
Type Human Untagged ORF Clone
Target Symbol TXNRD3
Synonyms TGR; TR2; TR2IT1; TRXR3; TXNRD3IT1; TXNRD3NB; TXNRD3NT1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC230340 representing NM_001173513
Red=Cloning site Blue=ORF Green=Tags(s)



CTGGAGCGGTCGCCGCCGCAGTCGCCCGGGCCGGGAAAGGCGGGCGATGCCCCCAACCGCCGCTCGGGCC
ATGTCCGAGGGGCGCGCGTGTTGTCGCCGCCGGGGCGCCGTGCCCGCCTGTCGTCCCCCGGGCCCAGCCG
CTCGTCCGAGGCCCGCGAGGAGCTGCGCCGCCACCTCGTGGGCCTCATCGAGCGCAGCCGGGTGGTGATC
TTCAGCAAGAGCTACTGTCCCCATAGTACTCGGGTGAAAGAACTCTTTTCTTCTTTGGGAGTCGAATGTA
ATGTCTTGGAACTTGATCAAGTTGATGATGGGGCCAGGGTTCAAGAAGTGCTGTCAGAAATCACTAATCA
GAAAACTGTGCCCAATATTTTCGTGAATAAAGTGCATGTAGGTGGATGTGACCAAACTTTCCAGGCATAT
CAGAGTGGTTTGTTACAGAAGCTCCTTCAGGAAGATTTGGCATATGATTATGATCTCATCATCATCGGTG
GTGGTTCTGGAGGCCTTTCATGTGCGAAGGAAGCTGCCATTTTGGGAAAGAAAGTTATGGTGCTAGACTT
TGTTGTCCCGTCACCTCAGGGCACATCCTGGGGTCTTGGTGGCACTTGTGTAAATGTAGGTTGTATTCCT
AAGAAATTGATGCATCAGGCTGCCCTTTTGGGGCAGGCATTATGTGACTCAAGGAAATTTGGCTGGGAAT
ATAATCAACAAGTGAGGCACAACTGGGAGACAATGACAAAAGCGATTCAGAACCACATCAGCTCTCTAAA
CTGGGGCTACAGGTTGTCTCTGAGGGAAAAGGCTGTGGCCTATGTCAATTCCTATGGAGAATTTGTTGAA
CATCATAAAATAAAGGCAACCAATAAAAAAGGACAGGAGACTTATTATACTGCTGCACAGTTTGTCATAG
CAACGGGTGAAAGGCCACGGTATTTAGGAATCCAAGGAGATAAAGAATACTGTATTACTAGTGATGACCT
TTTTTCTCTGCCTTATTGCCCTGGCAAAACATTAGTGGTGGGTGCCTCTTATGTTGCCCTGGAGTGTGCA
GGGTTTCTGGCTGGCTTTGGCCTAGATGTCACAGTTATGGTACGCTCAATCCTTCTCCGTGGCTTCGACC
AAGAAATGGCAGAAAAAGTGGGTTCCTACATGGAGCAGCATGGTGTGAAGTTCCTACGGAAATTCATACC
TGTGATGGTTCAACAGTTGGAGAAAGGTTCACCTGGAAAGCTGAAAGTGTTGGCTAAATCCACTGAAGGA
ACAGAAACAATTGAAGGAGTCTATAACACAGTTTTGTTAGCTATTGGTCGTGACTCCTGTACAAGGAAAA
TAGGCTTGGAGAAGATTGGTGTCAAAATTAATGAGAAGAGTGGAAAAATACCTGTAAATGATGTGGAACA
GACCAATGTGCCATATGTCTATGCTGTTGGTGATATTTTGGAGGATAAGCCAGAGCTCACTCCTGTCGCC
ATACAGTCAGGCAAGCTGCTAGCTCAGAGACTTTTTGGGGCCTCTTTAGAAAAGATATATCATACTTTGT
TCTGGCCTCTTGAATGGACAGTAGCTGGCAGAGAGAACAACACTTGTTACGCAAAGATAATCTGCAATAA
ATTCGACCATGATCGGGTGATAGGATTTCATATTCTTGGACCAAACGCCGGTGAGGTTACCCAAGGATTT
GCAGCTGCAATGAAATGTGGGCTCACAAAACAGCTACTTGATGACACCATTGGAATTCACCCCACATGTG
GGGAGGTGTTCACGACTTTGGAAATCACAAAGTCGTCAGGACTAGACATCACTCAGAAAGGCTGCTGAGG
C

Protein Sequence
>RC230340 representing NM_001173513
Red=Cloning site Green=Tags(s)



TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001173513
ORF Size 1821 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone encodes a selenoprotein containing the rare amino acid selenocysteine (Sec). Sec is encoded by UGA codon, which normally signals translational termination. Expression of this clone is not guaranteed due to the nature of selenoproteins.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001173513.1, NP_001166984.1
RefSeq ORF 1824 bp
Locus ID 114112
UniProt ID Q86VQ6
Cytogenetics 3q21.3
Summary The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes the third TrxR, which unlike the other two isozymes, contains an additional N-terminal glutaredoxin (Grx) domain, and shows highest expression in testis. The Grx domain allows this isozyme to participate in both Trx and glutathione systems. It functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene. Experimental evidence suggests the use of a non-AUG (CUG) codon as a translation initiation codon (PMID:20018845). provided by RefSeq, Aug 2017
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Documents
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"NM_001173513" in other vectors (2)
SKU Description Size Price
RG230340 TXNRD3 (GFP-tagged) - Human thioredoxin reductase 3 (TXNRD3), transcript variant 2, (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below) 10 ug
$744.00
SC328978 TXNRD3 (untagged)-Human thioredoxin reductase 3 (TXNRD3) transcript variant 2 (10ug), (Note: selenocysteine protein, Internal stop codon present. see reference data summary below) 10 ug
$1,133.00

Plasmid Map for RC230340

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC230340
Circular map for RC230340

file-alt Citations

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