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DGCR2 (NM_001173533) Human Tagged ORF Clone

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SKU
RC230205
DGCR2 (Myc-DDK-tagged)-Human DiGeorge syndrome critical region gene 2 (DGCR2), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $474.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol DGCR2
Synonyms DGS-C; IDD; LAN; SEZ-12
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC230205 representing NM_001173533
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCCCAAGGCAGACAGCGGCGCCTTCCTGCTGCTCTTCCTGCTCGTGCTCACTGTCACCGAGCCGC
TGCGGCCAGAAGTGACCGGGGAGGTGCGTCCTCATCATGGGAAGGAGGCTGTGGATCCGCGGCAGGGGCG
GGCCAGAGGAGGCGACCCTTCGCACTTCCACGCGGTGAACGTGGCGCAGCCCGTTCGCTTCAGCAGTTTC
CTAGGGAAGTGCCCGACAGGGTGGCACCACTACGAAGGCACGGCCAGCTGCTACCGGGTCTACCTGAGCG
GGGAGAACTACTGGGATGCCGCGCAGACCTGCCAGCGCCTGAATGGCTCTCTCGCCACCTTCTCCACTGA
CCAGGAGCTGCGCTTTGTCCTGGCCCAGGAATGGGACCAGCCCGAGCGGAGCTTTGGTTGGAAGGACCAG
CGCAAGTTGTGGGTTGGCTATCAGTATGTTATCACTGGCCGGAACCGCTCCTTGGAAGGTCGCTGGGAGG
TGGCATTCAAAGGCTCTTCAGAGGTGTTCCTGCCCCCAGACCCCATCTTTGCCTCGGCCATGTCTGAGAA
CGACAACGTGTTCTGTGCCCAGCTTCAGTGCTTCCATTTCCCCACCCTGCGGCACCACGACCTCCACAGC
TGGCACGCCGAGAGCTGCTACGAGAAGTCTTCATTTCTGTGTAAAAGAAGTCAAACATGTGTTGACATCA
AGGACAACGTGGTGGATGAAGGGTTCTACTTCACCCCTAAGGGGGACGACCCATGCCTGAGCTGCACCTG
CCATGGAGGGGAGCCTGAGATGTGTGTGGCTGCTCTCTGTGAGAGGCCCCAGGGCTGCCAACAGTACCGC
AAGGACCCCAAAGAGTGCTGCAAGTTCATGTGTCTGGACCCAGATGGCAACAGTCTGTTTGACTCCATGG
CCAGCGGGATGCGCCTGGTCGTCAGCTGCATCTCCTCCTTCCTCATCCTGTCACTGCTGCTCTTCATGGT
CCACCGGCTGCGCCAGCGGCGCCGGGAGCGCATCGAGTCCCTGATTGGAGCAAACTTGCACCACTTCAAC
CTCGGCCGCAGGATCCCTGGCTTTGATTACGGCCCAGACGGGTTTGGCACGGGCCTCACGCCGCTGCATC
TTTCTGACGACGGAGAGGGTGGGACTTTCCATTTCCACGACCCTCCACCTCCCTACACGGCATACAAGTA
CCCGGACATCGGCCAGCCCGACGACCCTCCGCCGCCCTACGAGGCCTCCATCCACCCGGACAGTGTGTTC
TATGACCCTGCAGACGATGATGCTTTTGAGCCTGTGGAGGTCAGCCTGCCAGCCCCTGGGGATGGTGGGA
GTGAAGGTGCATTACTCCGGCGCCTGGAGCAGCCTCTGCCCACTGCGGGGGCCTCTCTGGCAGACCTGGA
AGACTCTGCCGACAGCAGCAGCGCCCTGCTCGTGCCCCCTGACCCTGCCCAGAGCGGGAGCACCCCAGCT
GCAGAGGCACTGCCAGGGGGTGGCCGCCACAGCCGCAGCTCCCTCAATACTGTGGTG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC230205 representing NM_001173533
Red=Cloning site Green=Tags(s)

MVPKADSGAFLLLFLLVLTVTEPLRPEVTGEVRPHHGKEAVDPRQGRARGGDPSHFHAVNVAQPVRFSSF
LGKCPTGWHHYEGTASCYRVYLSGENYWDAAQTCQRLNGSLATFSTDQELRFVLAQEWDQPERSFGWKDQ
RKLWVGYQYVITGRNRSLEGRWEVAFKGSSEVFLPPDPIFASAMSENDNVFCAQLQCFHFPTLRHHDLHS
WHAESCYEKSSFLCKRSQTCVDIKDNVVDEGFYFTPKGDDPCLSCTCHGGEPEMCVAALCERPQGCQQYR
KDPKECCKFMCLDPDGNSLFDSMASGMRLVVSCISSFLILSLLLFMVHRLRQRRRERIESLIGANLHHFN
LGRRIPGFDYGPDGFGTGLTPLHLSDDGEGGTFHFHDPPPPYTAYKYPDIGQPDDPPPPYEASIHPDSVF
YDPADDDAFEPVEVSLPAPGDGGSEGALLRRLEQPLPTAGASLADLEDSADSSSALLVPPDPAQSGSTPA
AEALPGGGRHSRSSLNTVV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001173533
ORF Size 1527 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001173533.1, NP_001167004.1
RefSeq ORF 1530 bp
Locus ID 9993
UniProt ID P98153, Q8IWC8
Cytogenetics 22q11.21
Protein Families Druggable Genome, Transmembrane
MW 56.7 kDa
Summary Deletions of the 22q11.2 have been associated with a wide range of developmental defects (notably DiGeorge syndrome, velocardiofacial syndrome, conotruncal anomaly face syndrome and isolated conotruncal cardiac defects) classified under the acronym CATCH 22. The DGCR2 gene encodes a novel putative adhesion receptor protein, which could play a role in neural crest cells migration, a process which has been proposed to be altered in DiGeorge syndrome. Alternative splicing results in multiple transcript variants.provided by RefSeq, May 2010
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Documents
Resources
"NM_001173533" in other vectors (4)
SKU Description Size Price
RC230205L3 Lenti-ORF clone of DGCR2 (Myc-DDK-tagged)-Human DiGeorge syndrome critical region gene 2 (DGCR2), transcript variant 2 10 ug
$774.00
RC230205L4 Lenti-ORF clone of DGCR2 (mGFP-tagged)-Human DiGeorge syndrome critical region gene 2 (DGCR2), transcript variant 2 10 ug
$774.00
RG230205 DGCR2 (tGFP-tagged) - Human DiGeorge syndrome critical region gene 2 (DGCR2), transcript variant 2 10 ug
$674.00
SC328843 DGCR2 (untagged)-Human DiGeorge syndrome critical region gene 2 (DGCR2) transcript variant 2 10 ug
$522.00

Plasmid Map for RC230205

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC230205
Circular map for RC230205

file-alt Citations

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