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UBAP1 (NM_001171203) Human Tagged ORF Clone

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SKU
RC230186
UBAP1 (Myc-DDK-tagged)-Human ubiquitin associated protein 1 (UBAP1), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $467.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "UBAP1 or NM_001171203"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol UBAP1
Synonyms NAG20; SPG80; UAP; UBAP; UBAP-1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC230186 representing NM_001171203
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTTCTAAGAAGTTGGGTGCAGATTTTCATGGGACTTTCAGTTACCTTGATGATGTCCCATTTAAGA
CAGGAGACAAATTCAAAACACCAGCTAAAGTTGGTCTACCTATTGGCTTCTCCTTGCCTGATTGTTTGCA
GGTTGTCAGAGAAGTACAGTATGACTTCTCTTTGGAAAAGAAAACCATTGAGTGGGCTGAAGAGATTAAG
AAAATCGAAGAAGCCGAGCGGGAAGCAGAGTGCAAAATTGCGGAAGCAGAAGCTAAAGTGAATTCTAAGA
GTGGCCCAGAGGGCGATAGCAAAATGAGCTTCTCCAAGACTCACAGTACAGCCACAATGCCACCTCCTAT
TAACCCCATCCTCGCCAGCTTGCAGCACAACAGCATCCTCACACCAACTCGGGTCAGCAGTAGTGCCACG
AAACAGAAAGTTCTCAGCCCACCTCACATAAAGGCGGATTTCAATCTTGCTGACTTTGAGTGTGAAGAAG
ACCCATTTGATAATCTGGAGTTAAAAACTATTGATGAGAAGGAAGAGCTGAGAAATATTCTGGTAGGAAC
CACTGGACCCATTATGGCTCAGTTATTGGACAATAACTTGCCCAGGGGAGGCTCTGGGTCTGTGTTACAG
GATGAGGAGGTCCTGGCATCCTTGGAACGGGCAACCCTAGATTTCAAGCCTCTTCATAAACCCAATGGCT
TTATAACCTTACCACAGTTGGGCAACTGTGAAAAGATGTCACTGTCTTCCAAAGTGTCCCTCCCCCCTAT
ACCTGCAGTAAGCAATATCAAATCCCTGTCTTTCCCCAAACTTGACTCTGATGACAGCAATCAGAAGACA
GCCAAGCTGGCGAGCACTTTCCATAGCACATCCTGCCTCCGCAATGGCACGTTCCAGAATTCCCTAAAGC
CTTCCACCCAAAGCAGTGCCAGTGAGCTCAATGGGCATCACACTCTTGGGCTTTCAGCTTTGAACTTGGA
CAGTGGCACAGAGATGCCAGCCCTGACATCCTCCCAGATGCCTTCCCTCTCTGTTTTGTCTGTGTGCACA
GAGGAATCATCACCTCCAAATACTGGTCCCACGGTCACCCCTCCTAATTTCTCAGTGTCACAAGTGCCCA
ACATGCCCAGCTGTCCCCAGGCCTATTCTGAACTGCAGATGCTGTCCCCCAGCGAGCGGCAGTGTGTGGA
GACGGTGGTCAACATGGGCTACTCGTACGAGTGTGTCCTCAGAGCCATGAAGAAGAAAGGAGAGAATATT
GAGCAGATTCTCGACTATCTCTTTGCACATGGACAGCTTTGTGAGAAGGGCTTCGACCCTCTTTTAGTGG
AAGAGGCTCTGGAAATGCACCAGTGTTCAGAAGAAAAGATGATGGAGTTTCTTCAGTTAATGAGCAAATT
TAAGGAGATGGGCTTTGAGCTGAAAGACATTAAGGAAGTTTTGCTATTACACAACAATGACCAGGACAAT
GCTTTGGAAGACCTCATGGCTCGGGCAGGAGCCAGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC230186 representing NM_001171203
Red=Cloning site Green=Tags(s)

MASKKLGADFHGTFSYLDDVPFKTGDKFKTPAKVGLPIGFSLPDCLQVVREVQYDFSLEKKTIEWAEEIK
KIEEAEREAECKIAEAEAKVNSKSGPEGDSKMSFSKTHSTATMPPPINPILASLQHNSILTPTRVSSSAT
KQKVLSPPHIKADFNLADFECEEDPFDNLELKTIDEKEELRNILVGTTGPIMAQLLDNNLPRGGSGSVLQ
DEEVLASLERATLDFKPLHKPNGFITLPQLGNCEKMSLSSKVSLPPIPAVSNIKSLSFPKLDSDDSNQKT
AKLASTFHSTSCLRNGTFQNSLKPSTQSSASELNGHHTLGLSALNLDSGTEMPALTSSQMPSLSVLSVCT
EESSPPNTGPTVTPPNFSVSQVPNMPSCPQAYSELQMLSPSERQCVETVVNMGYSYECVLRAMKKKGENI
EQILDYLFAHGQLCEKGFDPLLVEEALEMHQCSEEKMMEFLQLMSKFKEMGFELKDIKEVLLLHNNDQDN
ALEDLMARAGAS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001171203
ORF Size 1506 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001171203.3
RefSeq ORF 1509 bp
Locus ID 51271
UniProt ID Q9NZ09
Cytogenetics 9p13.3
MW 55.5 kDa
Summary This gene is a member of the UBA domain family, whose members include proteins having connections to ubiquitin and the ubiquitination pathway. The ubiquitin associated domain is thought to be a non-covalent ubiquitin binding domain consisting of a compact three helix bundle. This particular protein originates from a gene locus in a refined region on chromosome 9 undergoing loss of heterozygosity in nasopharyngeal carcinoma (NPC). Taking into account its cytogenetic location, this UBA domain family member is being studies as a putative target for mutation in nasopharyngeal carcinomas. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jan 2010
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Documents
Resources
"NM_001171203" in other vectors (4)
SKU Description Size Price
RC230186L3 Lenti-ORF clone of UBAP1 (Myc-DDK-tagged)-Human ubiquitin associated protein 1 (UBAP1), transcript variant 2 10 ug
$767.00
RC230186L4 Lenti-ORF clone of UBAP1 (mGFP-tagged)-Human ubiquitin associated protein 1 (UBAP1), transcript variant 2 10 ug
$767.00
RG230186 UBAP1 (tGFP-tagged) - Human ubiquitin associated protein 1 (UBAP1), transcript variant 2 10 ug
$667.00
SC328824 UBAP1 (untagged)-Human ubiquitin associated protein 1 (UBAP1) transcript variant 2 10 ug
$515.00

Plasmid Map for RC230186

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC230186
Circular map for RC230186

file-alt Citations

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