LRRC34 (NM_001172779) Human Tagged ORF Clone

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SKU
RC230129
LRRC34 (Myc-DDK-tagged)-Human leucine rich repeat containing 34 (LRRC34), transcript variant 2
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LRRC34
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC230129 representing NM_001172779
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGCGCAGCCGCCGCGGCCAGTGGGTGAGAGGAGCATGGGGAGCTCCCGGGAGGCCGCCCGTGCGC
CGGCCAGGTCCCCGGCTTGGGCCTCCACTCAGGCCAGTACTCCGGGCGCGGCCCTGGCGGTCCAGCGCGA
ATCTCCAGAATCTGGTCTCCAGAAACATTATTCTAATCTGTGTATGGAAAAATCCCAGAAAATTAATCCT
TTTATATTGCATATACTCCAAGAAGTGGATGAAGAAATTAAAAAGGGGCTAGCAGCAGGAATCACATTAA
ACATTGCTGGTAACAATCGCTTAGTGCCAGTAGAAAGAGTTACAGGTGAAGATTTTTGGATTCTTTCCAA
AATTTTAAAGAATTGTCTGTATATTAATGGTTTGGATGTTGGATATAACCTTCTATGTGATGTTGGTGCA
TACTATGCTGCGAAACTGCTTCAGAAACAACTTAATCTCATTTACTTAAACCTCATGTTTAATGATATTG
GGCCCGAAGGTGGAGAATTGATTGCTAAAGTGCTACATAAGAATCGGACTCTGAAATACCTAAGAATGAC
TGGAAACAAAATTGAAAATAAAGGTGGAATGTTTTTTGCTGCAATGCTGCAAATTAATTCATCATTAGAG
AAATTAGATCTGGGTGACTGTGATCTGGGAATGCAAAGTGTGATAGCATTTGCTACAGTACTAACTCAAA
ACCAAGCAATTAAGGCAATAAACCTAAACCGACCTATACTGTACAGTGAACAGGAAGAGTCTACAGTCCA
TGTAGGCCGCATGTTGAAAGAAAATCACTGTCTTGTTGCACTACACATGTGTAAGCATGATATAAAAAAC
AGTGGTATACAACAGTTATGTGATGCACTGTATCTGAACAGTAGCCTGCGCTACCTTGATGTCAGCTGCA
ACAAAATAACTCATGATGGAATGGTGTATTTGGCTGATGTACTGAAAAGCAACACTACCCTGGAAGTAAT
AGATCTTTCCTTTAACAGAATAGAAAATGCAGGCGCAAACTATCTCAGTGAAACTCTTACTTCACACAAC
AGGAGTCTTAAAGCGTTGTCAGTAGTCAGCAACAACATAGAGGGAGAAGGACTTGTTGCACTTTCACAAT
CAATGAAAACAAATCTCACTTTCTCTCATATCTACATTTGGGGAAACAAATTTGATGAGGCTACGTGTAT
AGCATATTCAGACTTAATTCAAATGGGTTGTCTAAAACCAGACAATACAGATGTGGAGCCATTTGTGGTA
GATGGACGTGTATATCTTGCAGAAGTCTCCAATGGCCTTAAAAAGCATTATTATTGGACATCAACTTATG
GAGAATCTTATGACCACTCATCTAATGCAGGTTTTGCTCTTGTTCCAGTAGGTCAACAGCCA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC230129 representing NM_001172779
Red=Cloning site Green=Tags(s)

MAAQPPRPVGERSMGSSREAARAPARSPAWASTQASTPGAALAVQRESPESGLQKHYSNLCMEKSQKINP
FILHILQEVDEEIKKGLAAGITLNIAGNNRLVPVERVTGEDFWILSKILKNCLYINGLDVGYNLLCDVGA
YYAAKLLQKQLNLIYLNLMFNDIGPEGGELIAKVLHKNRTLKYLRMTGNKIENKGGMFFAAMLQINSSLE
KLDLGDCDLGMQSVIAFATVLTQNQAIKAINLNRPILYSEQEESTVHVGRMLKENHCLVALHMCKHDIKN
SGIQQLCDALYLNSSLRYLDVSCNKITHDGMVYLADVLKSNTTLEVIDLSFNRIENAGANYLSETLTSHN
RSLKALSVVSNNIEGEGLVALSQSMKTNLTFSHIYIWGNKFDEATCIAYSDLIQMGCLKPDNTDVEPFVV
DGRVYLAEVSNGLKKHYYWTSTYGESYDHSSNAGFALVPVGQQP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001172779
ORF Size 1392 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001172779.2
RefSeq ORF 1395 bp
Locus ID 151827
UniProt ID Q8IZ02
Cytogenetics 3q26.2
MW 51.7 kDa
Summary Highly expressed in stem cells where it may be involved in regulation of pluripotency. In embryonic stem cells (ESCs), important for normal expression of the pluripotency regulators POU5F1/OCT4 and KLF4. Also important for expression of the ectodermal marker gene NES and the endodermal marker gene GATA4. Promotes stem cell proliferation in vitro.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001172779" in other vectors (4)
SKU Description Size Price
RC230129L3 Lenti ORF clone of Human leucine rich repeat containing 34 (LRRC34), transcript variant 2, Myc-DDK-tagged 10 ug
$757.00
RC230129L4 Lenti ORF clone of Human leucine rich repeat containing 34 (LRRC34), transcript variant 2, mGFP tagged 10 ug
$757.00
RG230129 LRRC34 (tGFP-tagged) - Human leucine rich repeat containing 34 (LRRC34), transcript variant 2 10 ug
$657.00
SC328767 LRRC34 (untagged)-Human leucine rich repeat containing 34 (LRRC34) transcript variant 2 10 ug
$503.00

Plasmid Map for RC230129

Download sequence for

Cloning scheme for RC230129

Circular map for RC230129

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