PP11 (ENDOU) (NM_001172439) Human Tagged ORF Clone

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SKU
RC230028
ENDOU (Myc-DDK-tagged)-Human endonuclease, polyU-specific (ENDOU), transcript variant 1
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PP11
Synonyms P11; PP11; PRSS26
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC230028 representing NM_001172439
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGGGCCTGCATCTCCCTGGTATTGGCCGTGCTGTGTGGCCTGGCCTGGGCTGGAAAAATAGAATCCT
GTGCATCTCGATGTAATGAGAAATTTAACCGGGACGCTGCCTGCCAGTGTGACCGCCGGTGTCTCTGGCA
TGGGAACTGCTGTGAAGACTATGAACATCTGTGTACTGAGGACCACAAAGAGTCAGAGCCATTGCCACAG
CTGGAGGAAGAGACAGAAGAGGCCCTCGCCAGCAACTTGTACTCGGCACCCACCTCCTGCCAGGGCCGCT
GCTACGAAGCCTTTGACAAGCACCACCAATGTCACTGCAATGCCCGCTGCCAAGAGTTTGGGAACTGCTG
CAAGGATTTTGAGAGCCTGTGTAGTGACCACGAGGTCTCCCACAGCAGTGATGCCATAACAAAAGAGGAG
ATTCAGAGCATCTCTGAGAAGATCTACAGGGCAGACACCAACAAAGCCCAGAAGGAAGACATCGTTCTCA
ATAGCCAAAACTGCATCTCCCCGTCAGAGACCAGAAACCAAGTGGATCGCTGCCCAAAGCCACTCTTCAC
TTATGTCAATGAGAAGCTGTTCTCCAAGCCCACCTATGCAGCCTTCATCAACCTCCTCAACAACTACCAG
CGGGCAACAGGCCATGGGGAGCACTTCAGTGCCCAGGAGCTGGCCGAGCAGGACGCCTTCCTCAGAGAGA
TCATGAAGACAGCAGTCATGAAGGAGCTCTACAGCTTCCTCCATCACCAGAATCGCTATGGCTCAGAGCA
AGAGTTTGTCGATGACTTGAAGAACATGTGGTTTGGGCTCTATTCAAGAGGCAATGAAGAGGGGGACTCG
AGTGGCTTTGAACATGTCTTCTCAGGTGAGGTAAAAAAAGGCAAGGTTACTGGCTTCCATAACTGGATCC
GCTTCTACCTGGAGGAGAAGGAGGGTCTGGTTGACTATTACAGTCACATCTACGATGGGCCTTGGGATTC
TTACCCCGATGTGCTGGCAATGCAGTTCAACTGGGACGGCTACTATAAGGAAGTGGGCTCTGCTTTCATC
GGCAGCAGCCCTGAGTTTGAGTTTGCACTCTACTCCCTGTGCTTCATCGCCAGGCCAGGCAAAGTGTGCC
AGTTAAGCCTGGGAGGATATCCCTTAGCTGTCCGGACATATACCTGGGACAAGTCCACCTATGGGAATGG
CAAGAAGTACATCGCCACAGCCTACATAGTGTCTTCCACC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC230028 representing NM_001172439
Red=Cloning site Green=Tags(s)

MRACISLVLAVLCGLAWAGKIESCASRCNEKFNRDAACQCDRRCLWHGNCCEDYEHLCTEDHKESEPLPQ
LEEETEEALASNLYSAPTSCQGRCYEAFDKHHQCHCNARCQEFGNCCKDFESLCSDHEVSHSSDAITKEE
IQSISEKIYRADTNKAQKEDIVLNSQNCISPSETRNQVDRCPKPLFTYVNEKLFSKPTYAAFINLLNNYQ
RATGHGEHFSAQELAEQDAFLREIMKTAVMKELYSFLHHQNRYGSEQEFVDDLKNMWFGLYSRGNEEGDS
SGFEHVFSGEVKKGKVTGFHNWIRFYLEEKEGLVDYYSHIYDGPWDSYPDVLAMQFNWDGYYKEVGSAFI
GSSPEFEFALYSLCFIARPGKVCQLSLGGYPLAVRTYTWDKSTYGNGKKYIATAYIVSST

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001172439
ORF Size 1230 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001172439.2
RefSeq ORF 1233 bp
Locus ID 8909
UniProt ID P21128
Cytogenetics 12q13.11
Protein Families Protease
MW 47.3 kDa
Summary This gene encodes a protein with endoribonuclease activity that binds polyuridine-enriched single-stranded RNA. This gene was initially characterized based on its high expression in placenta but was mischaracterized as a serine protease. In mouse, this gene promotes tolerance to self-antigens by regulating B cell activation-induced cell death (AICD). The protein may be useful as a tumor marker. Multiple alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene. provided by RefSeq, Jul 2020
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Documents
Resources
"NM_001172439" in other vectors (4)
SKU Description Size Price
RC230028L3 Lenti ORF clone of Human endonuclease, polyU-specific (ENDOU), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC230028L4 Lenti ORF clone of Human endonuclease, polyU-specific (ENDOU), transcript variant 1, mGFP tagged 10 ug
$757.00
RG230028 ENDOU (tGFP-tagged) - Human endonuclease, polyU-specific (ENDOU), transcript variant 1 10 ug
$657.00
SC328666 ENDOU (untagged)-Human endonuclease polyU-specific (ENDOU) transcript variant 1 10 ug
$503.00

Plasmid Map for RC230028

Download sequence for

Cloning scheme for RC230028

Circular map for RC230028

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