TMPRSS4 (NM_001173552) Human Tagged ORF Clone

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SKU
RC229994
TMPRSS4 (Myc-DDK-tagged)-Human transmembrane protease, serine 4 (TMPRSS4), transcript variant 5
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol TMPRSS4
Synonyms CAP2; CAPH2; MT-SP2; TMPRSS3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC229994 representing NM_001173552
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATCCTGACAGTGATCAACCTCTGAACAGCCTCGTCAAGGTGATTCTGGATAAATACTACTTCCTCT
GCGGGCAGCCTCTCCACTTCATCCCGAGGAAGCAGCTGTGTGACGGAGAGCTGGACTGTCCCTTGGGGGA
GGACGAGGAGCACTGTGTCAAGAGCTTCCCCGAAGGGCCTGCAGTGGCAGTCCGCCTCTCCAAGGACCGA
TCCACACTGCAGGTGCTGGACTCGGCCACAGGGAACTGGTTCTCTGCCTGTTTCGACAACTTCACAGAAG
CTCTCGCTGAGACAGCCTGTAGGCAGATGGGCTACAGCAGCAAACCCACTTTCAGAGCTGTGGAGATTGG
CCCAGACCAGGATCTGGATGTTGTTGAAATCACAGAAAACAGCCAGGAGCTTCGCATGCGGAACTCAAGT
GGGCCCTGTCTCTCAGGCTCCCTGGTCTCCCTGCACTGTCTTGCCTGTGGGAAGAGCCTGAAGACCCCCC
GTGTGGTGGGTGGGGAGGAGGCCTCTGTGGATTCTTGGCCTTGGCAGGTCAGCATCCAGTACGACAAACA
GCACGTCTGTGGAGGGAGCATCCTGGACCCCCACTGGGTCCTCACGGCAGCCCACTGCTTCAGGAAACAT
ACCGATGTGTTCAACTGGAAGGTGCGGGCAGGCTCAGACAAACTGGGCAGCTTCCCATCCCTGGCTGTGG
CCAAGATCATCATCATTGAATTCAACCCCATGTACCCCAAAGACAATGACATCGCCCTCATGAAGCTGCA
GTTCCCACTCACTTTCTCAGGCACAGTCAGGCCCATCTGTCTGCCCTTCTTTGATGAGGAGCTCACTCCA
GCCACCCCACTCTGGATCATTGGATGGGGCTTTACGAAGCAGAATGGAGGGAAGATGTCTGACATACTGC
TGCAGGCGTCAGTCCAGGTCATTGACAGCACACGGTGCAATGCAGACGATGCGTACCAGGGGGAAGTCAC
CGAGAAGATGATGTGTGCAGGCATCCCGGAAGGGGGTGTGGACACCTGCCAGGGTGACAGTGGTGGGCCC
CTGATGTACCAATCTGACCAGTGGCATGTGGTGGGCATCGTTAGTTGGGGCTATGGCTGCGGGGGCCCGA
GCACCCCAGGAGTATACACCAAGGTCTCAGCCTATCTCAACTGGATCTACAATGTCTGGAAGGCTGAGCT
G


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC229994 representing NM_001173552
Red=Cloning site Green=Tags(s)

MDPDSDQPLNSLVKVILDKYYFLCGQPLHFIPRKQLCDGELDCPLGEDEEHCVKSFPEGPAVAVRLSKDR
STLQVLDSATGNWFSACFDNFTEALAETACRQMGYSSKPTFRAVEIGPDQDLDVVEITENSQELRMRNSS
GPCLSGSLVSLHCLACGKSLKTPRVVGGEEASVDSWPWQVSIQYDKQHVCGGSILDPHWVLTAAHCFRKH
TDVFNWKVRAGSDKLGSFPSLAVAKIIIIEFNPMYPKDNDIALMKLQFPLTFSGTVRPICLPFFDEELTP
ATPLWIIGWGFTKQNGGKMSDILLQASVQVIDSTRCNADDAYQGEVTEKMMCAGIPEGGVDTCQGDSGGP
LMYQSDQWHVVGIVSWGYGCGGPSTPGVYTKVSAYLNWIYNVWKAEL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001173552
ORF Size 1191 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001173552.1, NP_001167023.1
RefSeq ORF 1194 bp
Locus ID 56649
UniProt ID Q9NRS4
Cytogenetics 11q23.3
Protein Families Druggable Genome, Protease, Transmembrane
MW 44.2 kDa
Summary This gene encodes a member of the serine protease family. Serine proteases are known to be involved in a variety of biological processes, whose malfunction often leads to human diseases and disorders. This gene was identified as a gene overexpressed in pancreatic carcinoma. The encoded protein is membrane bound with a N-terminal anchor sequence and a glycosylated extracellular region containing the serine protease domain. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_001173552" in other vectors (6)
SKU Description Size Price
RC229994L1 Lenti ORF clone of Human transmembrane protease, serine 4 (TMPRSS4), transcript variant 5, Myc-DDK-tagged 10 ug
$757.00
RC229994L2 Lenti ORF clone of Human transmembrane protease, serine 4 (TMPRSS4), transcript variant 5, mGFP tagged 10 ug
$757.00
RC229994L3 Lenti ORF clone of Human transmembrane protease, serine 4 (TMPRSS4), transcript variant 5, Myc-DDK-tagged 10 ug
$757.00
RC229994L4 Lenti ORF clone of Human transmembrane protease, serine 4 (TMPRSS4), transcript variant 5, mGFP tagged 10 ug
$757.00
RG229994 TMPRSS4 (tGFP-tagged) - Human transmembrane protease, serine 4 (TMPRSS4), transcript variant 5 10 ug
$657.00
SC328632 TMPRSS4 (untagged)-Human transmembrane protease serine 4 (TMPRSS4) transcript variant 5 10 ug
$503.00

Plasmid Map for RC229994

Download sequence for

Cloning scheme for RC229994

Circular map for RC229994

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