RNF32 (NM_001184996) Human Tagged ORF Clone

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SKU
RC229940
RNF32 (Myc-DDK-tagged)-Human ring finger protein 32 (RNF32), transcript variant 1
  $457.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol RNF32
Synonyms FKSG33; HSD15; LMBR2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC229940 representing NM_001184996
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTTAAAAAATAAGGGTCACTCATCTAAGAAAGATAACTTGGCAGTCAATGCAGTTGCTTTACAAGATC
ACATTTTACATGATCTTCAACTTCGAAATCTTTCAGTTGCAGATCATTCTAAGACACAAGTACAAAAGAA
AGAGAACAAATCTCTAAAAAGAGATACAAAGGCAATAATAGATACTGGACTTAAAAAAACTACACAGTGC
CCCAAACTAGAAGACTCAGAAAAAGAATATGTTCTTGATCCCAAACCGCCGCCGTTGACTTTGGCACAGA
AGTTGGGCCTCATTGGGCCTCCACCACCTCCACTGTCATCAGATGAATGGGAGAAGGTGAAACAGCGCTC
TCTCCTGCAAGGGGACTCCGTGCAACCATGCCCCATCTGTAAAGAAGAATTCGAGCTTCGTCCTCAGGTG
CTGCTTTCATGCTCCCATGTGTTCCACAAAGCATGTCTTCAGGCTTTTGAAAAGTTCACAAATAAGAAAA
CCTGTCCTCTCTGTAGAAAGAACCAGTATCAAACCCGAGTGATACACGATGGGGCCCGCCTGTTCAGAAT
CAAGTGTGTGACCAGAATCCAAGCCTACTGGAGAGGATGTGTTGTTAGAAAGTGGTACAGAAACCTGAGG
AAAACAGTACCTCCCACAGATGCCAAGTTAAGAAAAAAATTCTTTGAAAAAAAGTTCACAGAAATCAGCC
ACCGCATCCTGTGCTCATACAACACCAACATTGAAGAGCTCTTTGCAGAAATCGATCAGTGCTTGGCCAT
AAATCGAAGTGTTCTTCAGCAGTTGGAAGAAAAATGTGGCCATGAGATCACAGAAGAGGAATGGGAGAAA
ATCCAAGTGCAGGCTCTGCGCCGGGAGACCCACGAGTGCTCCATCTGCCTGGCCCCTCTCTCCGCTGCTG
GCGGTCAGCGCGTGGGTGCAGGCAGGCGTTCCAGAGAGATGGCCCTCCTGTCCTGCTCACATGTGTTCCA
CCATGCGTGTCTGCTGGCACTAGAGGAGTTCTCCGTGGGAGACAGGCCTCCTTTCCATGCCTGTCCTCTC
TGCCGCTCCTGCTACCAGAAGAAGATTCTTGAATGT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC229940 representing NM_001184996
Red=Cloning site Green=Tags(s)

MLKNKGHSSKKDNLAVNAVALQDHILHDLQLRNLSVADHSKTQVQKKENKSLKRDTKAIIDTGLKKTTQC
PKLEDSEKEYVLDPKPPPLTLAQKLGLIGPPPPPLSSDEWEKVKQRSLLQGDSVQPCPICKEEFELRPQV
LLSCSHVFHKACLQAFEKFTNKKTCPLCRKNQYQTRVIHDGARLFRIKCVTRIQAYWRGCVVRKWYRNLR
KTVPPTDAKLRKKFFEKKFTEISHRILCSYNTNIEELFAEIDQCLAINRSVLQQLEEKCGHEITEEEWEK
IQVQALRRETHECSICLAPLSAAGGQRVGAGRRSREMALLSCSHVFHHACLLALEEFSVGDRPPFHACPL
CRSCYQKKILEC

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001184996
ORF Size 1086 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001184996.1, NP_001171925.1
RefSeq ORF 1089 bp
Locus ID 140545
UniProt ID Q9H0A6, A0A024RD87
Cytogenetics 7q36.3
Protein Families Druggable Genome
MW 42 kDa
Summary The protein encoded by this gene contains two RING ring finger motifs. RING finger motifs are present in a variety of functionally distinct proteins and are known to be involved in protein-DNA or protein-protein interactions. This gene was found to be expressed during spermatogenesis, most likely in spermatocytes and/or in spermatids. Alternative splicing of this gene results in multiple transcript variants. provided by RefSeq, Apr 2015
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Documents
Resources
"NM_001184996" in other vectors (4)
SKU Description Size Price
RC229940L3 Lenti ORF clone of Human ring finger protein 32 (RNF32), transcript variant 1, Myc-DDK-tagged 10 ug
$757.00
RC229940L4 Lenti ORF clone of Human ring finger protein 32 (RNF32), transcript variant 1, mGFP tagged 10 ug
$757.00
RG229940 RNF32 (tGFP-tagged) - Human ring finger protein 32 (RNF32), transcript variant 1 10 ug
$657.00
SC328578 RNF32 (untagged)-Human ring finger protein 32 (RNF32) transcript variant 1 10 ug
$503.00

Plasmid Map for RC229940

Download sequence for

Cloning scheme for RC229940

Circular map for RC229940

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