PNPLA5 (NM_001177675) Human Tagged ORF Clone

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SKU
RC229871
PNPLA5 (Myc-DDK-tagged)-Human patatin-like phospholipase domain containing 5 (PNPLA5), transcript variant 2
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PNPLA5
Synonyms dJ388M5; dJ388M5.4; GS2L
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC229871 representing NM_001177675
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGCTTCTTAGAGGAGGAGGGCAGATGGAACCTGTCCTTCTCCGGCGCCGGCTACCTGGGCGCCCACC
ACGTGGGCGCCACCGAATGCCTGCGCCAGCGAGCCCCGCGCCTCCTCCAGGGCGCCCGCCGCATCTACGG
TTCCTCGTCTGGGGCGCTCAACGCAGTCAGCATCGTCTGCGGCAAGTCGGTCGACTGCCCCTCCACCATC
ACGGTGTCGCCCTTCCATGGGACAGTGGACATCTGCCCCCAGAGCACCTCCCCCAACCTGCATGAGCTGA
ACGTCTTCAACTTCAGCTTCCAAATCTCCACTGAGAACTTCTTCCTGGGGCTCATATGTCTCATACCCCC
CAGCCTCGAGGTAGTGGCCGACAACTGCAGACAAGGCTACCTGGATGCCCTGAGGTTCCTGGAGAGACGT
GGACTCACCAAGGAACCAGTGCTATGGACGCTGGTGTCTAAGGAACCCCCAGCCCCGGCTGACGGAAACT
GGGATGCTGGCTGTGACCAACGCTGGAAGGGGGGCCTGTCTCTCAACTGGAAAGTGCCCCATGTGCAAGT
CAAGGATGTACCCAACTTTGAGCAGCTCTCACCAGAGCTGGAGGCTGCACTGAAGAAAGCATGTACGAGG
GATCCCAGCCGGTGGGCCCGCTTCTGGCACTCGGGGCCTGGACAGGTGCTGACGTACCTGCTGCTACCCT
GCACACTGCCCTTCGAGTACATCTACTTCCGCAGCAGAAGGTTGGTGGTGTGGCTGCCCGATGTGCCGGC
GGACTTGTGGTGGATGCAGGGCCTGCTGAGGAACATGGCCCTCGAGGTTTTCTCCAGGACCAAGGCCCAG
CTCCTTGGGCCCATCAGCCCTCCGGCCACTCGCGTCCTGGAAACAAGCCCCCTCCAACCCCAGATAGCTC
CTCATAGAGAGGAGCTCGGGCCCACCCACCAGGCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC229871 representing NM_001177675
Red=Cloning site Green=Tags(s)

MGFLEEEGRWNLSFSGAGYLGAHHVGATECLRQRAPRLLQGARRIYGSSSGALNAVSIVCGKSVDCPSTI
TVSPFHGTVDICPQSTSPNLHELNVFNFSFQISTENFFLGLICLIPPSLEVVADNCRQGYLDALRFLERR
GLTKEPVLWTLVSKEPPAPADGNWDAGCDQRWKGGLSLNWKVPHVQVKDVPNFEQLSPELEAALKKACTR
DPSRWARFWHSGPGQVLTYLLLPCTLPFEYIYFRSRRLVVWLPDVPADLWWMQGLLRNMALEVFSRTKAQ
LLGPISPPATRVLETSPLQPQIAPHREELGPTHQA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001177675
ORF Size 945 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001177675.1, NP_001171146.1
RefSeq ORF 948 bp
Locus ID 150379
UniProt ID Q7Z6Z6
Cytogenetics 22q13.31
MW 35.6 kDa
Summary This gene is a member of the patatin-like phospholipase family; its encoded protein has been shown to inhibit transacylation. Multiple transcript variants encoding different isoforms have been found for this gene.provided by RefSeq, Apr 2010
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Documents
Resources
"NM_001177675" in other vectors (4)
SKU Description Size Price
RC229871L3 Lenti-ORF clone of PNPLA5 (Myc-DDK-tagged)-Human patatin-like phospholipase domain containing 5 (PNPLA5), transcript variant 2 10 ug
$600.00
RC229871L4 Lenti-ORF clone of PNPLA5 (mGFP-tagged)-Human patatin-like phospholipase domain containing 5 (PNPLA5), transcript variant 2 10 ug
$600.00
RG229871 PNPLA5 (tGFP-tagged) - Human patatin-like phospholipase domain containing 5 (PNPLA5), transcript variant 2 10 ug
$500.00
SC328509 PNPLA5 (untagged)-Human patatin-like phospholipase domain containing 5 (PNPLA5) transcript variant 2 10 ug
$330.00

Plasmid Map for RC229871

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Cloning scheme for RC229871

Circular map for RC229871

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