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CCDC148 (NM_001171637) Human Tagged ORF Clone

  • TrueORF®
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SKU
RC229849
CCDC148 (Myc-DDK-tagged)-Human coiled-coil domain containing 148 (CCDC148), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
$289.00 $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol CCDC148
Synonyms MGC125588; MGC125590
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC229849 representing NM_001171637
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAATCTGAAATAAAATCCCTTCTCAATGAAGAGAACATTGGAAATGAATGTCTTTGTGACCTTACAA
ATTTTGAGCAAGAGCTATCAGAACAACAGTGCACATATCTTAAAAATGTAATAAATCCTATTCAGCAGCT
GAGAGCAGATCTAAAATACAGACAGCATCACACTTTGCAGCATTCACACCCACATATTGAGTTTAACTCC
ATGAAAGTATTGGAAGAGGTTGACTTTGTGAAGAAACAATTGAAAACTGTCTTTGAAAGACTTAGGCTGG
AGCAACAGAGAATAGAAAATGATCTTTCAGACTGGAGTATAAAGATTCTAGATCATTCTTTGGAAGAAAA
GACTAACCCGCTTAGTGAACTGCCCATTGAATTAGAAAGTTTGGAATGCCCATACCCTGATTTGAAATCT
TCAATTCTCAGTGAGTTCTATAAGTTTACACAGAAATATCAGAAGAAGCTTCAAGACTTTAATCTGCAGT
TGGAAGACATATACAGAAACTGTCAACTAAGTGAAGAAGACCACTGGATTTACCAGGCTATTTTGGATCA
GTACCCTGGAGATCTCTTTGGAAGAAGGACTCTGTATCTGGACATGTTACAAAGATATTTTCCTCACAAA
TCTAGGCATGATTTGGTTGAACACGAGAAATATTGTGACCAATATCGCTTTGCTATAGAGCAGCAAAATA
TCCTGATATCAAATTGGAATAAAAATAAGAAAGACTTTATACAGAAGGCTGTGCTGACACTCACTGAGGC
TTGTGCAACACATGAAATGGAGAGCATGTTGGCTAAGGACAAGAAAAAGCAACAGGAATTGTGTGCTGAT
CTGAAAGCCAAGTCTCAGGTATGTCTTTATCAGCAGCATGAAAATGGACTAATACAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC229849 representing NM_001171637
Red=Cloning site Green=Tags(s)

MESEIKSLLNEENIGNECLCDLTNFEQELSEQQCTYLKNVINPIQQLRADLKYRQHHTLQHSHPHIEFNS
MKVLEEVDFVKKQLKTVFERLRLEQQRIENDLSDWSIKILDHSLEEKTNPLSELPIELESLECPYPDLKS
SILSEFYKFTQKYQKKLQDFNLQLEDIYRNCQLSEEDHWIYQAILDQYPGDLFGRRTLYLDMLQRYFPHK
SRHDLVEHEKYCDQYRFAIEQQNILISNWNKNKKDFIQKAVLTLTEACATHEMESMLAKDKKKQQELCAD
LKAKSQVCLYQQHENGLIQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001171637
ORF Size 897 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001171637.1, NP_001165108.1
RefSeq ORF 900 bp
Locus ID 130940
Cytogenetics 2q24.1
MW 36.2 kDa
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Documents
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"NM_001171637" in other vectors (4)
SKU Description Size Price
RC229849L3 Lenti ORF clone of Human coiled-coil domain containing 148 (CCDC148), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC229849L4 Lenti ORF clone of Human coiled-coil domain containing 148 (CCDC148), transcript variant 2, mGFP tagged 10 ug
$600.00
RG229849 CCDC148 (tGFP-tagged) - Human coiled-coil domain containing 148 (CCDC148), transcript variant 2 10 ug
$500.00
SC328487 CCDC148 (untagged)-Human coiled-coil domain containing 148 (CCDC148) transcript variant 2 10 ug
$330.00

Plasmid Map for RC229849

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC229849
Circular map for RC229849

file-alt Citations

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