LOC136242 (PRSS37) (NM_001171951) Human Tagged ORF Clone

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SKU
RC229734
PRSS37 (Myc-DDK-tagged)-Human protease, serine, 37 (PRSS37), transcript variant 2
  $300.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol LOC136242
Synonyms TRYX2
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC229734 representing NM_001171951
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAATATGTCTTCTATTTGGGTGTCCTCGCTGGGACATTTTTCTTTGCTGACTCATCTGTTCAGAAAG
AAGACCCTGCTCCCTATTTGGTGTACCTCAAGTCTCACTTCAACCCCTGTGTGGGCGTCCTCATCAAACC
CAGCTGGGTGCTGGCCCCAGCTCACTGCTATTTACCAAATCTGAAAGTGATGCTGGGAAATTTCAAGAGC
AGAGTCAGAGACGGTACTGAACAGACAATTAACCCCATTCAGATCGTCCGCTACTGGAACTACAGTCATA
GCGCCCCACAGGATGACCTCATGCTCATCAAGCTGGCTAAGCCTGCCATGCTCAATCCCAAAGTCCAGCC
CCTTACCCTCGCCACCACCAATGTCAGGCCAGGCACTGTCTGTCTACTCTCAGGTTTGGACTGGAGCCAA
GAAAACAGCCGACACCCTGACTTGCGGCAGAACCTGGAGGCCCCCGTGATGTCTGATCGAGAATGCCAAA
AAACAGAACAAGGAAAAAGCCACAGGAATTCCTTATGTGTGAAATTTGTGAAAGTATTCAGCCGAATTTT
TGGGGAGGTGGCCGTTGCTACTGTCATCTGCAAAGACAAGCTCCAGGGAATCGAGGTGGGGCACTTCATG
GGAGGGGACGTCGGCATCTACACCAATGTTTACAAATATGTATCCTGGATTGAGAACACTGCTAAGGACA
AG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC229734 representing NM_001171951
Red=Cloning site Green=Tags(s)

MKYVFYLGVLAGTFFFADSSVQKEDPAPYLVYLKSHFNPCVGVLIKPSWVLAPAHCYLPNLKVMLGNFKS
RVRDGTEQTINPIQIVRYWNYSHSAPQDDLMLIKLAKPAMLNPKVQPLTLATTNVRPGTVCLLSGLDWSQ
ENSRHPDLRQNLEAPVMSDRECQKTEQGKSHRNSLCVKFVKVFSRIFGEVAVATVICKDKLQGIEVGHFM
GGDVGIYTNVYKYVSWIENTAKDK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001171951
ORF Size 702 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001171951.1, NP_001165422.1
RefSeq ORF 705 bp
Locus ID 136242
UniProt ID B7ZMK3
Cytogenetics 7q34
Protein Families Druggable Genome
MW 26.8 kDa
Summary Plays a role in male fertility (By similarity). May have a role in sperm migration or binding to zona-intact eggs (By similarity). Involved in the activation of the proacrosin/acrosin system (PubMed:27649891).UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001171951" in other vectors (4)
SKU Description Size Price
RC229734L3 Lenti ORF clone of Human protease, serine, 37 (PRSS37), transcript variant 2, Myc-DDK-tagged 10 ug
$600.00
RC229734L4 Lenti ORF clone of Human protease, serine, 37 (PRSS37), transcript variant 2, mGFP tagged 10 ug
$600.00
RG229734 PRSS37 (tGFP-tagged) - Human protease, serine, 37 (PRSS37), transcript variant 2 10 ug
$500.00
SC328372 PRSS37 (untagged)-Human protease serine 37 (PRSS37) transcript variant 2 10 ug
$330.00

Plasmid Map for RC229734

Download sequence for

Cloning scheme for RC229734

Circular map for RC229734

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