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HDHD1A (PUDP) (NM_001178136) Human Tagged ORF Clone

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SKU
RC229645
HDHD1 (Myc-DDK-tagged)-Human haloacid dehalogenase-like hydrolase domain containing 1 (HDHD1), transcript variant 4
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $300.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol HDHD1A
Synonyms DXF68S1E; FAM16AX; GS1; HDHD1; HDHD1A
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC229645 representing NM_001178136
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGCGCCCCCGCAGCCCGTCACCCACCTCATCTTTGACATGGACGGACTTCTTCTGGATACTGAAC
GGCTGTATTCAGTGGTGTTTCAAGAAATATGTAATCGCTATGACAAGAAATACAGCTGGGATGTAAAGTC
CCTGGTTATGGGGGCGGAGAAACTCATCATCCACCTGCGGAAACATGGCATCCCCTTTGCACTGGCCACC
AGCTCGGGGTCCGCGTCGTTCGATATGAAGACAAGCCGCCACAAGGAGTTCTTCAGCTTGTTTTCCCACA
TTGTGCTGGGAGATGACCCCGAAGTGCAGCATGGCAAGCCAGACCCGGACATCTTCCTAGCTTGTGCCAA
GAGGTTCTCTCCCCCTCCTGCTATGGAGAAGTGCCTTGTCTTTGAAGATGCTCCCAATGGGGTGGAGGCG
GCCCTGGCAGCTGGGATGCAGGTGGTCATGGTTCCTGACGGAAACTTGAGCCGAGATCTGACAACAAAGG
CCACCCTGGTGCTGAATTCCCTGCAGGACTTCCAGCCCGAGCTGTTTGGTTTGCCCTCCTATGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC229645 representing NM_001178136
Red=Cloning site Green=Tags(s)

MAAPPQPVTHLIFDMDGLLLDTERLYSVVFQEICNRYDKKYSWDVKSLVMGAEKLIIHLRKHGIPFALAT
SSGSASFDMKTSRHKEFFSLFSHIVLGDDPEVQHGKPDPDIFLACAKRFSPPPAMEKCLVFEDAPNGVEA
ALAAGMQVVMVPDGNLSRDLTTKATLVLNSLQDFQPELFGLPSYE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001178136
ORF Size 555 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001178136.2
RefSeq ORF 558 bp
Locus ID 8226
UniProt ID Q08623
Cytogenetics Xp22.31
MW 21 kDa
Summary This gene encodes a member of the haloacid dehalogenase-like (HAD) hydrolase superfamily. The encoded protein has no known biological function. This gene has a pseudogene on chromosome 1. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. provided by RefSeq, Jul 2010
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"NM_001178136" in other vectors (4)
SKU Description Size Price
RC229645L3 Lenti ORF clone of Human haloacid dehalogenase-like hydrolase domain containing 1 (HDHD1), transcript variant 4, Myc-DDK-tagged 10 ug
$600.00
RC229645L4 Lenti ORF clone of Human haloacid dehalogenase-like hydrolase domain containing 1 (HDHD1), transcript variant 4, mGFP tagged 10 ug
$600.00
RG229645 HDHD1 (tGFP-tagged) - Human haloacid dehalogenase-like hydrolase domain containing 1 (HDHD1), transcript variant 4 10 ug
$500.00
SC328283 HDHD1 (untagged)-Human haloacid dehalogenase-like hydrolase domain containing 1A (HDHD1A) transcript variant 4 10 ug
$330.00

Plasmid Map for RC229645

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC229645
Circular map for RC229645

file-alt Citations

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