MST1 (NM_020998) Human Tagged ORF Clone
SKU
RC229352
MST1 (Myc-DDK-tagged)-Human macrophage stimulating 1 (hepatocyte growth factor-like) (MST1)
$664.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | MST1 |
| Synonyms | D3F15S2; DNF15S2; HGFL; MSP; NF15S2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_020998 |
| ORF Size | 2175 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_020998.3, NP_066278.3 |
| RefSeq ORF | 2178 bp |
| Locus ID | 4485 |
| UniProt ID | P26927, G3XAK1, Q53GN8 |
| Cytogenetics | 3p21.31 |
| Domains | KR, PAN, PAN_AP, Tryp_SPc |
| Protein Families | Druggable Genome |
| MW | 78.4 kDa |
| Summary | The protein encoded by this gene contains four kringle domains and a serine protease domain, similar to that found in hepatic growth factor. Despite the presence of the serine protease domain, the encoded protein may not have any proteolytic activity. The receptor for this protein is RON tyrosine kinase, which upon activation stimulates ciliary motility of ciliated epithelial lung cells. This protein is secreted and cleaved to form an alpha chain and a beta chain bridged by disulfide bonds. provided by RefSeq, Jan 2010 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_020998" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC208248 | MST1 (Myc-DDK-tagged)-Human macrophage stimulating 1 (hepatocyte growth factor-like) (MST1) | 10 ug |
$716.00
|
|
| RC229352L3 | Lenti ORF clone of Human macrophage stimulating 1 (hepatocyte growth factor-like) (MST1), Myc-DDK-tagged | 10 ug |
$964.00
|
|
| RC229352L4 | Lenti ORF clone of Human macrophage stimulating 1 (hepatocyte growth factor-like) (MST1), mGFP tagged | 10 ug |
$964.00
|
|
| RG208248 | MST1 (tGFP-tagged) - Human macrophage stimulating 1 (hepatocyte growth factor-like) (MST1) | 10 ug |
$916.00
|
|
| RG229352 | MST1 (tGFP-tagged) - Human macrophage stimulating 1 (hepatocyte growth factor-like) (MST1) | 10 ug |
$864.00
|
|
| SC113074 | MST1 (untagged)-Human macrophage stimulating 1 (hepatocyte growth factor-like) (MST1) | 10 ug |
$652.00
|
|
| SC327987 | MST1 (untagged)-Human macrophage stimulating 1 (hepatocyte growth factor-like) (MST1) | 10 ug |
$665.00
|
|
Citations
Molecular Mechanisms of Tyrosine Kinase Inhibitor Resistance Induced by Membranous/Cytoplasmic/Nuclear Translocation of Epidermal Growth Factor Receptor
Rong, Liang, Han et al
J Thorac Oncol (2019) 14 (10), 1766-1783 DOI: 10.1016/j.jtho.2019.06.014
Rong, Liang, Han et al
J Thorac Oncol (2019) 14 (10), 1766-1783 DOI: 10.1016/j.jtho.2019.06.014
AI Summary: OriGene products—specifically pCMV6-Entry, YAP1, SIK2, and MST1 plasmids—were used for transfection and gene expression studies to investigate the molecular mechanisms of tyrosine kinase inhibitor resistance related to EGFR localization in lung adenocarcinoma.
Overexpression of Mst1 reduces gastric cancer cell viability by repressing the AMPK-Sirt3 pathway and activating mitochondrial fission
Yao, Yan
Onco Targets Ther (2018) 11, 8465-8479 DOI: 10.2147/OTT.S180851
Yao, Yan
Onco Targets Ther (2018) 11, 8465-8479 DOI: 10.2147/OTT.S180851
AI Summary: OriGene’s pCMV6-Kan/Neo Mst1 plasmid was used to overexpress Mst1 in gastric cancer cells, enabling the study of its effects on cell viability and mitochondrial function.
Snippet: … The pCMV6-Kan/Neo Mst1 plasmids (1,247 bp;forward, 5′-CATGGTTAGTCTTGCATTGTGC-3′ and reverse, 5′-GGTAGGTCTCCATTAT CTTCCA-3′) were obtained from OriGene Technologies, Inc. Then;The pCMV6-Kan/Neo Mst1 plasmids (1,247 bp;forward, 5′-CATGGTTAGTCTTGCATTGTGC-3′ and reverse, 5′-GGTAGGTCTCCATTATCTTCCA-3′) were obtained from OriGene Technologies, Inc. … After 48 hours, the supernatant was collected to obtain the Mst1 adenovirus (Ad-Mst1), which was transfected into AGS cells in Opti-MEM media supplemented with Lipofectamine 2000 according to the manufacturer’s protocol.33 Transfection was carried out for 48 hours under 5% CO2 at 37°C
A ROR1-HER3-lncRNA signalling axis modulates the Hippo-YAP pathway to regulate bone metastasis
Li, Wang, Xing et al
Nat. Cell Biol. (2017) 19 (2), 106-119 DOI: 10.1038/ncb3464
Li, Wang, Xing et al
Nat. Cell Biol. (2017) 19 (2), 106-119 DOI: 10.1038/ncb3464
Hippo signaling protein MST1 regulates osteoclast differentiation by interacting with integrin linked kinase (ILK) and modulating actin structures
Huang, Li
Archives of Biological Sciences (2016)
Huang, Li
Archives of Biological Sciences (2016)
Snippet: siRNA sequences targeting MST1, YAP, TAZ, TEAD1, LATS2 or ILK (Santacruz Biotechnology, USA) were transfected and evaluated after 48 h. For overexpression, cells were either transiently transfected with an empty vector or as full-length constructs of MST1 or ILK (Origene, USA). RANKL treatments were induced 48 h post siRNA treatment or expression of transfected plamid in all of the experiments
Showing 1-4 of 4 papers.
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