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GOLGA8B (NM_001023567) Human Tagged ORF Clone

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SKU
RC229308
GOLGA8B (Myc-DDK-tagged)-Human golgin A8 family, member B (GOLGA8B), transcript variant 1
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $561.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GOLGA8B
Synonyms GOLGA5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC229308 representing NM_001023567
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAGAAGAAACTGGACAGAGTAAATTAGCTGCAGCCAAGAAAAAGTTCAAAGAATATTGGCAGAGAA
ACCGCCCTGGTGTTCCAGCAGCAGCGAAGAGGAACACGAAAGCAAATGGCAGTAGCCCTGAGACGGCCGC
TTCTGGTGGTTGCCACTCATCTGAGGCTTCCTCCTCCGCCTCCTCCTCTCTGCATGCGCGTCAGAGCCCG
TGCCAAGAACAAGCAGCAGTCCTGAACTCGAGGTCCATAAAAATCAGTCGACTGAATGACACCATCAAAT
CTTTGAAACAACAGAAGAAACAAGTGGAACATCAGCTGGAAGAAGAAAAGAAAGCAAACAATGAGAAACA
GAAAGCTGAAAGGGAGCTAGAGGGTCAAATCCAGAGATTGAACACAGAGAAAAAGAAACTAAATACGGAC
CTGTATCACATGAAACATTCTCTCAGATACTTTGAAGAAGAGTCCAAGGATCTGGCCGGCCGCCTGCAAC
GTTCATCGCAGCGTATAGGAGAGTTAGAGTGGTCTCTCTGTGCTGTCGCCGCCACACAGAAGAAGAAGCC
GGATGGGTTCTCGAGCCGCAGTAAAGCACTTCTCAAGCGGCAGTTAGAGCAGTCCATACGGGAGCAGATA
CTGCTGAAAGGACACGTGACACAGTTGAAGGAGTCGCTTAAAGAAGTCCAGCTGGAGAGAGATCAATATG
CTGAACAAATAAAAGGAGAGAGGGCCCAGTGGCAGCAGAGGATGAGGAAAATGTCGCAGGAGGTTTGCAC
ATTGAAGGAGGAGAAGAAGCATGATACGCATCGGGTAGAGGAGCTGGAGAGGAGCTTGTCCAGACTCAAA
AACCAGATGGCTGAGCCACTGCCCCCGGATGCCCCAGCAGTGTCCTCTGAGGTGGAGCTGCAAGACCTGA
GGAAGGAGCTGGAGAGAGTGGCAGGAGAGCTCCAGGCTCAGGTGGAAAACAATCAGTGCATCAGTCTCCT
GAACCGTGGGCAAAAGGAGAGGCTTCGCGAGCAGGAGGAGAGGCTTCAGGAGCAGCAGGAGAGGCTTCGG
GAACGGGAGAAGAGGCTTCAGCAGCTGGCCGAGCCACAGAGCGACTTGGAGGAGCTGAAGCACGAGAACA
AGAGCGCACTGCAGTTGGAGCAGCAAGTAAAGGAGCTGCAGGAGAAGCTGGGCCAGGTGATGGAGACGCT
CACCTCGGCCGAGAAGGAGCCAGAGGCAGCAGTCCCAGCCTCAGGGACTGGGGGCGAGTCTAGCGGCCTT
ATGGACCTCCTGGAGGAGAAGGCGGACCTGAGGGAGCATGTGGAGAAACTGGAACTTGGATTCATCCAGT
ACCGGAGAGAGAGATGCCATCAGAAAGTACATCGCCTTCTAACAGAGCCAGGGGACAGTGCCAAAGATGC
GTCACCGGGAGGAGGCCATCATCAGGCTGGCCCAGGACAAGGAGGAGAGGAAGGTGAAGCTGCTGGAGCT
GCAGGAGATGGTGTTGCGGCTTGTGGCAGCTACAGCGAGGGGCACGGCAAATTCCTGGCCGCTGCCCGGA
ACCCTGCTGCTGAACCCAGTCCAGGAGCCCCAGCCCCCCAGGAGCTCGGGGCTGCCGACAAGCATGGTGA
TCTTTGTGAGGCGAGCCTCACCAACAGCGTGGAGCCTGCACAAGGAGAAGCCAGGGAGGGTTCTTCCCAG
GACAACCCTACTGCACAGCCAGTCCTGCAGCTCCTTGGTGAGATGCAGGACCACCAGGAGCACCCAGGCT
TGGGCAGCAACTGCTGTGTGCCATGCTTTTGCTGGGCTTGGCTGCCGAGAAGAAGGAGA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC229308 representing NM_001023567
Red=Cloning site Green=Tags(s)

MAEETGQSKLAAAKKKFKEYWQRNRPGVPAAAKRNTKANGSSPETAASGGCHSSEASSSASSSLHARQSP
CQEQAAVLNSRSIKISRLNDTIKSLKQQKKQVEHQLEEEKKANNEKQKAERELEGQIQRLNTEKKKLNTD
LYHMKHSLRYFEEESKDLAGRLQRSSQRIGELEWSLCAVAATQKKKPDGFSSRSKALLKRQLEQSIREQI
LLKGHVTQLKESLKEVQLERDQYAEQIKGERAQWQQRMRKMSQEVCTLKEEKKHDTHRVEELERSLSRLK
NQMAEPLPPDAPAVSSEVELQDLRKELERVAGELQAQVENNQCISLLNRGQKERLREQEERLQEQQERLR
EREKRLQQLAEPQSDLEELKHENKSALQLEQQVKELQEKLGQVMETLTSAEKEPEAAVPASGTGGESSGL
MDLLEEKADLREHVEKLELGFIQYRRERCHQKVHRLLTEPGDSAKDASPGGGHHQAGPGQGGEEGEAAGA
AGDGVAACGSYSEGHGKFLAAARNPAAEPSPGAPAPQELGAADKHGDLCEASLTNSVEPAQGEAREGSSQ
DNPTAQPVLQLLGEMQDHQEHPGLGSNCCVPCFCWAWLPRRRR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001023567
ORF Size 1809 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001023567.5
RefSeq ORF 1812 bp
Locus ID 440270
UniProt ID A8MQT2
Cytogenetics 15q14
MW 67.1 kDa
Summary May be involved in maintaining Golgi structure.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001023567" in other vectors (5)
SKU Description Size Price
RC229308L3 Lenti ORF clone of Human golgin A8 family, member B (GOLGA8B), transcript variant 1, Myc-DDK-tagged 10 ug
$861.00
RC229308L4 Lenti ORF clone of Human golgin A8 family, member B (GOLGA8B), transcript variant 1, mGFP tagged 10 ug
$861.00
RG229308 GOLGA8B (tGFP-tagged) - Human golgin A8 family, member B (GOLGA8B), transcript variant 1 10 ug
$761.00
SC302218 GOLGA8B (untagged)-Human golgin A8 family, member B (GOLGA8B), transcript variant 1 10 ug
$618.00
SC327943 GOLGA8B (untagged)-Human golgi autoantigen golgin subfamily a 8B (GOLGA8B) transcript variant 1 10 ug
$618.00

Plasmid Map for RC229308

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC229308
Circular map for RC229308

file-alt Citations

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