RNF187 (NM_001010858) Human Tagged ORF Clone
$289.00
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | RNF187 |
| Synonyms | RACO-1; RACO1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_001010858 |
| ORF Size | 705 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_001010858.3 |
| RefSeq Size | 3133 bp |
| RefSeq ORF | 708 bp |
| Locus ID | 149603 |
| UniProt ID | Q5TA31 |
| Cytogenetics | 1q42.13 |
| MW | 26.2 kDa |
| Summary | E3 ubiquitin-protein ligase that acts as a coactivator of JUN-mediated gene activation in response to growth factor signaling via the MAP3K1 pathway, independently from MAPK8.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_001010858" in other vectors (7)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC229128L1 | Lenti ORF clone of Human ring finger protein 187 (RNF187), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC229128L2 | Lenti ORF clone of Human ring finger protein 187 (RNF187), mGFP tagged | 10 ug |
$600.00
|
|
| RC229128L3 | Lenti ORF clone of Human ring finger protein 187 (RNF187), Myc-DDK-tagged | 10 ug |
$600.00
|
|
| RC229128L4 | Lenti ORF clone of Human ring finger protein 187 (RNF187), mGFP tagged | 10 ug |
$600.00
|
|
| RG229128 | RNF187 (tGFP-tagged) - Human ring finger protein 187 (RNF187) | 10 ug |
$500.00
|
|
| SC325484 | RNF187 (untagged)-Human ring finger protein 187 (RNF187) | 10 ug |
$330.00
|
|
| SC327763 | RNF187 (untagged)-Human ring finger protein 187 (RNF187) | 10 ug |
$330.00
|
|
Citations
RBCK1 is an endogenous inhibitor for triple negative breast cancer via hippo/YAP axis
Li, Su, Ding et al
Cell Commun Signal (2022) 20 (1), 164 DOI: 10.1186/s12964-022-00963-8
Li, Su, Ding et al
Cell Commun Signal (2022) 20 (1), 164 DOI: 10.1186/s12964-022-00963-8
Cited by 17
AI Summary: OriGene contributed to this study by providing the Flag-RBCK1 plasmid (RC229128), which was used to investigate the role of RBCK1 in triple negative breast cancer progression via the Hippo/YAP signaling axis.
Snippet: …Plasmids and siRNA The Flag-RBCK1 plasmid was acquired from Origene (RC229128). …Plasmids were transfe;- The Flag-RBCK1 plasmid was acquired from Origene (RC229128). …
Regulation of P53 signaling in breast cancer by the E3 ubiquitin ligase RNF187
Li, Niu, Sun et al
Cell Death Dis (2022) 13 (2), 149 DOI: 10.1038/s41419-022-04604-3
Li, Niu, Sun et al
Cell Death Dis (2022) 13 (2), 149 DOI: 10.1038/s41419-022-04604-3
Cited by 16
AI Summary: In this study, OriGene products—specifically the RNF187 shRNA (OriGene, #TG313573) and P53 cDNA clone (OriGene, #SC117312)—were used to manipulate gene expression in breast cancer cells, enabling the authors to investigate how RNF187 regulates P53 signaling and contributes to breast cancer progression.
RACO-1 modulates Hippo signalling in oesophageal squamous cell carcinoma
Pang, Wang, Zhou et al
J. Cell. Mol. Med. (2020)
Pang, Wang, Zhou et al
J. Cell. Mol. Med. (2020)
Cited by 13
AI Summary: In this study, the Myc-RACO-1 plasmid from OriGene was used to investigate the role of RACO-1 in modulating Hippo signalling and YAP stability in oesophageal squamous cell carcinoma.
Snippet: The Myc‐RACO‐1 plasmid was acquired from Origene. The HA‐K48 and HA‐K63 Ubi plasmids were acquired from Henan Collaborative Innovation Center of Molecular Diagnosis and Laboratory Medicine.15 The Lipofectamine 2000 (11668019, Invitrogen) was used to transfect plasmid. Small interfering RNAs were used to knock down specific genes. The sequences of RACO‐1 siRNA were shown here: GUGAUGGACCGUAGGAAGAdT...
Regulation of Hippo signaling and triple negative breast cancer progression by an ubiquitin ligase RNF187
Wang, Kong, Su et al
Oncogenesis (2020) 9 (3), 36 DOI: 10.1038/s41389-020-0220-5
Wang, Kong, Su et al
Oncogenesis (2020) 9 (3), 36 DOI: 10.1038/s41389-020-0220-5
Cited by 59
AI Summary: In this study, OriGene products including RNF187 shRNA (TL313546) and YAP shRNA (TL313121) were used to specifically deplete RNF187 and YAP in triple negative breast cancer cells, enabling the authors to investigate the regulatory role of RNF187 in Hippo signaling and its impact on cancer cell migration and invasion.
Snippet: study provides a novel mechanism between RNF187 and Hippo signaling, which could be a promising strategy for TNBC treatment. …The activation of YAP functions to be a transcriptional cofactor and co-activates several tr;study provides a novel mechanism between RNF187 and Hippo signaling, which could be a promising strategy for TNBC treatment. …
Showing 1-4 of 4 papers.
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