GLOD5 (NM_001080489) Human Tagged ORF Clone

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SKU
RC229107
GLOD5 (Myc-DDK-tagged)-Human glyoxalase domain containing 5 (GLOD5)
  $150.00
In Stock*
Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol GLOD5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC229107 representing NM_001080489
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCTGCGCCATCTGCCCTCCAGGCTGCCAGTCAAGATGTGGGGCAGGACTTTGGAGAAACAGTCATGGA
GGGACAGCAGTCAGACCCCTCCCCCATGTCTTATCCGTAGACTTGACCACATCGTGATGACGGTGAAGAG
CATCAAAGACACCACCATGTTTTATTCCAAGATCCTGGGCATGGAGGTCATGACTTTTAAGGAAGACCGG
AAAGCACTGTGTTTTGGAGACCAGAAATTTAACCTCCACGAGGTGGGAAAGGAATTTGAACCCAAAGCCG
CTCACCCAGTTCCTGGCTCCCTGGACATATGTCTGATCACAGAGGTGCCTTTGGAGGAAATGATCCAGCA
CCTCAAGGCTTGTGATGTCCCTATTGAGGAGGGGCCAGTCCCCAGAACAGGGGCAAAAGGGCCTATCATG
TCCATCTACTTCCGAGACCCCGACAGAAATCTGATTGAGGTGTCCAACTACATCTCCTCG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC229107 representing NM_001080489
Red=Cloning site Green=Tags(s)

MLRHLPSRLPVKMWGRTLEKQSWRDSSQTPPPCLIRRLDHIVMTVKSIKDTTMFYSKILGMEVMTFKEDR
KALCFGDQKFNLHEVGKEFEPKAAHPVPGSLDICLITEVPLEEMIQHLKACDVPIEEGPVPRTGAKGPIM
SIYFRDPDRNLIEVSNYISS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001080489
ORF Size 480 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001080489.3
RefSeq ORF 483 bp
Locus ID 392465
UniProt ID A6NK44
Cytogenetics Xp11.23
MW 18.1 kDa
Summary This gene encodes a protein with a glyoxalase domain. provided by RefSeq, Sep 2011
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Documents
Resources
"NM_001080489" in other vectors (5)
SKU Description Size Price
RC229107L3 Lenti ORF clone of Human glyoxalase domain containing 5 (GLOD5), Myc-DDK-tagged 10 ug
$450.00
RC229107L4 Lenti ORF clone of Human glyoxalase domain containing 5 (GLOD5), mGFP tagged 10 ug
$450.00
RG229107 GLOD5 (tGFP-tagged) - Human glyoxalase domain containing 5 (GLOD5) 10 ug
$350.00
SC315678 GLOD5 (untagged)-Human glyoxalase domain containing 5 (GLOD5) 10 ug
$165.00
SC327742 GLOD5 (untagged)-Human glyoxalase domain containing 5 (GLOD5) 10 ug
$165.00

Plasmid Map for RC229107

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Cloning scheme for RC229107

Circular map for RC229107

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