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WDR46 (NM_001164267) Human Tagged ORF Clone

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SKU
RC228450
WDR46 (Myc-DDK-tagged)-Human WD repeat domain 46 (WDR46), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $518.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol WDR46
Synonyms BING4; C6orf11; FP221; UTP7
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC228450 representing NM_001164267
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGACAGCCCCCAAGCCGGGCAAGGATGTCCCGCCCAAGAAAGACAAACTTCAGACCAAGAGAAAGA
AACCCCGAGAATGGAAGAACCCGGAGTCCCAGCGCGGCTTGTCCGGGACCCAAGATCCATTCCCAGGCCC
CGCCCCCGTCCCTGTGGAAGTGGTCCAGAAGTTCTGTCGCATTGACAAATCCCGAAAGCTACCACATTCT
AAAGCCAAAACTCGAAGCCGACTTGAGGTGGCTGAAGCTGAGGAAGAGGAAACAAGTATCAAAGCTGCTC
GTTCTGAGCTGCTGCTTGCTGAAGAACCTGGGTTTCTGGAAGGGGAGGATGGGGAAGACACAGCAAAGAT
ATGCCAGGCTGACATTGTGGAGGCTGTGGACATTGCAAGTGCAGCCAAGCACTTTGACTTGAATCTGCGG
CAGTTTGGACCCTACAGACTAAACTACTCTCGAACTGGAAGACACCTGGCTTTTGGAGGGCGCCGAGGTC
ATGTGGCTGCCCTTGATTGGGTAACAAAGAAGCTTATGTGCGAGATCAACGTCATGGAGGCGGTGCGGGA
CATCCGGTTTCTCCATTCTGAGGCACTGCTTGCTGTTGCTCAGAACCGCTGGCTCCACATCTATGACAAT
CAGGGCATTGAGCTCCACTGTATCCGCCGCTGTGACCGAGTAACACGGCTTGAGTTCCTGCCCTTCCACT
TCCTCCTGGCTACAGCTTCAGAAACAGGGTTTCTAACCTACCTGGATGTGTCAGTGGGGAAGATTGTGGC
AGCTCTGAATGCTCGAGCTGGGCGGCTCGATGTTATGAGTCAGAACCCTTACAATGCCGTCATCCATCTC
GGACACAGCAATGGTACTGTGTCTTTATGGAGTCCAGCTATGAAGGAGCCACTGGCAAAGATTCTCTGTC
ATCGTGGTGGGGTCCGGGCTGTGGCAGTAGATTCTACAGGCACGTACATGGCCACCTCTGGCCTAGACCA
CCAGCTGAAGATCTTTGACTTGCGAGGGACGTACCAGCCTCTGAGCACTCGGACCCTGCCCCATGGAGCA
GGGCACCTGGCCTTCTCCCAGAGGGGACTGCTGGTGGCGGGAATGGGTGACGTTGTCAACATCTGGGCAG
GGCAGGGCAAGGCCAGCCCACCCTCCCTTGAACAGCCCTACCTCACCCACCGGCTCTCAGGCCCTGTGCA
TGGCCTTCAGTTCTGCCCCTTTGAAGATGTGCTGGGGGTGGGGCACACTGGGGGCATCACCAGCATGCTG
GTCCCTGGGGCCGGTGAGCCCAACTTCGATGGCCTGGAGAGTAATCCATACAGAAGCCGGAAGCAGCGCC
AGGAGTGGGAGGTGAAGGCCCTGCTAGAGAAGGTACCTGCAGAGCTTATTTGTCTGGACCCACGAGCCCT
GGCCGAGGTGGATGTCATCTCCCTGGAGCAGGGAAAGAAGGAGCAGATAGAGAGGCTGGGCTATGACCCG
CAGGCTAAGGCTCCCTTCCAGCCAAAGCCAAAGCAGAAGGGCCGCAGCTCCACGGCAAGCCTGGTGAAGA
GGAAGAGGAAGGTCATGGATGAGGAACACAGGGACAAGGTCCGGCAGAGCCTTCAGCAGCAGCATCATAA
GGAGGCGAAGGCCAAGCCCACGGGGGCCCGGCCATCTGCCCTGGACAGATTTGTGCGC

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC228450 representing NM_001164267
Red=Cloning site Green=Tags(s)

METAPKPGKDVPPKKDKLQTKRKKPREWKNPESQRGLSGTQDPFPGPAPVPVEVVQKFCRIDKSRKLPHS
KAKTRSRLEVAEAEEEETSIKAARSELLLAEEPGFLEGEDGEDTAKICQADIVEAVDIASAAKHFDLNLR
QFGPYRLNYSRTGRHLAFGGRRGHVAALDWVTKKLMCEINVMEAVRDIRFLHSEALLAVAQNRWLHIYDN
QGIELHCIRRCDRVTRLEFLPFHFLLATASETGFLTYLDVSVGKIVAALNARAGRLDVMSQNPYNAVIHL
GHSNGTVSLWSPAMKEPLAKILCHRGGVRAVAVDSTGTYMATSGLDHQLKIFDLRGTYQPLSTRTLPHGA
GHLAFSQRGLLVAGMGDVVNIWAGQGKASPPSLEQPYLTHRLSGPVHGLQFCPFEDVLGVGHTGGITSML
VPGAGEPNFDGLESNPYRSRKQRQEWEVKALLEKVPAELICLDPRALAEVDVISLEQGKKEQIERLGYDP
QAKAPFQPKPKQKGRSSTASLVKRKRKVMDEEHRDKVRQSLQQQHHKEAKAKPTGARPSALDRFVR

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001164267
ORF Size 1668 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001164267.2
RefSeq ORF 1671 bp
Locus ID 9277
UniProt ID O15213, A8K806, A0A1U9X8W1, B4DP15
Cytogenetics 6p21.32
Protein Families Druggable Genome
MW 61.6 kDa
Summary Scaffold component of the nucleolar structure. Required for localization of DDX21 and NCL to the granular compartment of the nucleolus.UniProtKB/Swiss-Prot Function
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Documents
Resources
"NM_001164267" in other vectors (4)
SKU Description Size Price
RC228450L3 Lenti-ORF clone of WDR46 (Myc-DDK-tagged)-Human WD repeat domain 46 (WDR46), transcript variant 2 10 ug
$818.00
RC228450L4 Lenti-ORF clone of WDR46 (mGFP-tagged)-Human WD repeat domain 46 (WDR46), transcript variant 2 10 ug
$818.00
RG228450 WDR46 (tGFP-tagged) - Human WD repeat domain 46 (WDR46), transcript variant 2 10 ug
$718.00
SC327085 WDR46 (untagged)-Human WD repeat domain 46 (WDR46) transcript variant 2 10 ug
$570.00

Plasmid Map for RC228450

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC228450
Circular map for RC228450

file-alt Citations

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