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PLA2G4C (NM_001159322) Human Tagged ORF Clone

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SKU
RC228444
PLA2G4C (Myc-DDK-tagged)-Human phospholipase A2, group IVC (cytosolic, calcium-independent) (PLA2G4C), transcript variant 2
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $513.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PLA2G4C
Synonyms CPLA2-gamma
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC228444 representing NM_001159322
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGCACACGCCCCAGACCCAGGCTCAGGAGGACTGAGAATTTTCTGACCGCAGTGCACCATGGGAAGA
AAGAAGAAAAGGCGGCCGTGGAGAGACGAAGACTTCATGTGCTGAAAGCTCTGAAGAAGCTAAGGATTGA
GGCTGATGAGGCCCCAGTTGTTGCTGTGCTGGGCTCAGGCGGAGGACTGCGGGCTCACATTGCCTGCCTT
GGGGTCCTGAGTGAGATGAAAGAACAGGGCCTGTTGGATGCCGTCACGTACCTCGCAGGGGTCTCTGGAT
CCACTTGGGCAATATCTTCTCTCTACACCAATGATGGTGACATGGAAGCTCTCGAGGCTGACCTGAAACA
TCGATTTACCCGACAGGAGTGGGACTTGGCTAAGAGCCTACAGAAAACCATCCAAGCAGCGAGGTCTGAG
AATTACTCTCTGACCGACTTCTGGGCCTACATGGTTATCTCTAAGCAAACCAGAGAACTGCCGGAGTCTC
ATTTGTCCAATATGAAGAAGCCCGTGGAAGAAGGGACACTACCCTACCCAATATTTGCAGCCATTGACAA
TGACCTGCAACCTTCCTGGCAGGAGGCAAGAGCACCAGAGACCTGGTTCGAGTTCACCCCTCACCACGCT
GGCTTCTCTGCACTGGGGGCCTTTGTTTCCATAACCCACTTCGGAAGCAAATTCAAGAAGGGAAGACTGG
TCAGAACTCACCCTGAGAGAGACCTGACTTTCCTGAGAGGTTTATGGGGAAGTGCTCTTGGTAACACTGA
AGTCATTAGGGAATACATTTTTGACCAGTTAAGGAATCTGACCCTGAAAGGTTTATGGAGAAGGGCTGTT
GCTAATGCTAAAAGCATTGGACACCTTATTTTTGCCCGATTACTGAGGCTGCAAGAAAGTTCACAAGGGG
AACATCCTCCCCCAGAAGATGAAGGCGGTGAGCCTGAACACACCTGGCTGACTGAGATGCTCGAGAATTG
GACCAGGACCTCCCTGGAAAAGCAGGAGCAGCCCCATGAGGACCCCGAAAGGAAAGGCTCACTCAGTAAC
TTGATGGATTTTGTGAAGAAAACAGGCATTTGCGCTTCAAAGTGGGAATGGGGGACCACTCACAACTTCC
TGTACAAACACGGTGGCATCCGGGACAAGATAATGAGCAGCCGGAAGCACCTCCACCTGGTGGATGCTGG
TTTAGCCATCAACACTCCCTTCCCACTCGTGCTGCCCCCGACGCGGGAGGTTCACCTCATCCTCTCCTTC
GACTTCAGTGCCGGAGATCCTTTCGAGACCATCCGGGCTACCACTGACTACTGCCGCCGCCACAAGATCC
CCTTTCCCCAAGTAGAAGAGGCTGAGCTGGATTTGTGGTCCAAGGCCCCCGCCAGCTGCTACATCCTGAA
AGGAGAAACTGGACCAGTGGTGATGCATTTTCCCCTGTTCAACATAGATGCCTGTGGAGGTGATATTGAG
GCATGGAGTGACACATACGACACATTCAAGCTTGCTGACACCTACACTCTAGATGTGGTGGTGCTACTCT
TGGCATTAGCCAAGAAGAATGTCAGGGAAAACAAGAAGAAGATCCTTAGAGAGTTGATGAACGTGGCCGG
GCTCTACTACCCGAAGGATAGTGCCCGAAGTTGCTGCTTGGCA

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC228444 representing NM_001159322
Red=Cloning site Green=Tags(s)

MRTRPRPRLRRTENFLTAVHHGKKEEKAAVERRRLHVLKALKKLRIEADEAPVVAVLGSGGGLRAHIACL
GVLSEMKEQGLLDAVTYLAGVSGSTWAISSLYTNDGDMEALEADLKHRFTRQEWDLAKSLQKTIQAARSE
NYSLTDFWAYMVISKQTRELPESHLSNMKKPVEEGTLPYPIFAAIDNDLQPSWQEARAPETWFEFTPHHA
GFSALGAFVSITHFGSKFKKGRLVRTHPERDLTFLRGLWGSALGNTEVIREYIFDQLRNLTLKGLWRRAV
ANAKSIGHLIFARLLRLQESSQGEHPPPEDEGGEPEHTWLTEMLENWTRTSLEKQEQPHEDPERKGSLSN
LMDFVKKTGICASKWEWGTTHNFLYKHGGIRDKIMSSRKHLHLVDAGLAINTPFPLVLPPTREVHLILSF
DFSAGDPFETIRATTDYCRRHKIPFPQVEEAELDLWSKAPASCYILKGETGPVVMHFPLFNIDACGGDIE
AWSDTYDTFKLADTYTLDVVVLLLALAKKNVRENKKKILRELMNVAGLYYPKDSARSCCLA

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_001159322
ORF Size 1653 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_001159322.1, NP_001152794.1
RefSeq ORF 1656 bp
Locus ID 8605
UniProt ID Q9UP65
Cytogenetics 19q13.33
MW 62.3 kDa
Summary This gene encodes a protein which is a member of the phospholipase A2 enzyme family which hydrolyzes glycerophospholipids to produce free fatty acids and lysophospholipids, both of which serve as precursors in the production of signaling molecules. The encoded protein has been shown to be a calcium-independent and membrane bound enzyme. Multiple transcript variants encoding different isoforms have been found for this gene. provided by RefSeq, Apr 2009
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"NM_001159322" in other vectors (4)
SKU Description Size Price
RC228444L3 Lenti-ORF clone of PLA2G4C (Myc-DDK-tagged)-Human phospholipase A2, group IVC (cytosolic, calcium-independent) (PLA2G4C), transcript variant 2 10 ug
$813.00
RC228444L4 Lenti-ORF clone of PLA2G4C (mGFP-tagged)-Human phospholipase A2, group IVC (cytosolic, calcium-independent) (PLA2G4C), transcript variant 2 10 ug
$813.00
RG228444 PLA2G4C (tGFP-tagged) - Human phospholipase A2, group IVC (cytosolic, calcium-independent) (PLA2G4C), transcript variant 2 10 ug
$713.00
SC327079 PLA2G4C (untagged)-Human phospholipase A2 group IVC (cytosolic calcium-independent) (PLA2G4C) transcript variant 2 10 ug
$565.00

Plasmid Map for RC228444

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC228444
Circular map for RC228444

file-alt Citations

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